We narrowed to 6,952 results for: crispr cas9 plasmids

1. pAAV-FLEXfrt-SaCas9-U6-sgGabrg2

   Plasmid

   #124871

   Purpose

   Mutagenesis of Gabrg2

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gabrg2 (Gabrg2 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPB-Ins-TRE3Gp-dCas9-Suntag-VP64-EF1Ap-Puro

   Plasmid

   #183409

   Purpose

   piggyBAC-based doxycycline-inducible dCas9-5xSuntag-VP64 CRISPR activation plasmid for enhancer and promoter activation in mammalian cells

   Depositor

   Azim Surani

   Article

   Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

   Insert

   dCas9-5xGCN5-P2A-scFV-sfGFP-VP64-GB1
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Tol2_B-act_dCas9-Vp64-T2A-Citrine

   Plasmid

   #200248

   Purpose

   Zebrafish transgenics; Plasmid encodes dCas9-Vp64 and could be stably integrated in the zebrafish genome using Tol2 transgenesis

   Depositor

   Tatjana Sauka-Spengler

   Article

   Pelea et al bioRxiv 2023.05.08.539738

   Insert

   dCas9-Vp64-T2A-Citrine
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZS157 CRISPEY RT/Cas9

   Plasmid

   #114454

   Purpose

   Yeast Integration Plasmid for galactose inducible Ec86-RT and SpCas9 expression

   Depositor

   Hunter Fraser

   Article

   Sharon et al Cell. 2018 Sep 18. pii: S0092-8674(18)31

   Insert

   SpCas9, Ec86-RT
   

   Use

   CRISPR

   Tags

   SV40-NLS

   Expression

   Yeast

   Mutation

   S. cerevisiae codon optimized

   Promoter

   GAL1-GAL10

   Available since

   Oct. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. eSpCas9(1.1)-Puro

   Plasmid

   #117686

   Purpose

   The plasmids pSpCas9 (BB)-2A-Puro (Addgene, #48139) and eSpCas9 (1.1) (Addgene, #71814) were combined to express both “enhanced specificity” Cas9 and puromycin resistance protein.

   Depositor

   Luc Leyns

   Article

   Liu et al Biochem Biophys Res Commun. 2019 Feb 14.

   Insert

   eSpCas9(1.1)
   

   Use

   CRISPR

   Tags

   PuroR (R166H)

   Expression

   Mammalian

   Mutation

   K848A, K1003A, R1060A

   Promoter

   U6

   Available since

   Feb. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-T7-TadCBEd-SpCas9-P2A-EGFP (BKS327)

   Plasmid

   #223123

   Purpose

   pCMV and pT7 Human expression plasmid for TadCBEd-SpCas9 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized TadCBEd-SpCas9-2xUGI-P2A-EGFP
   

   Use

   CRISPR

   Tags

   2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

   Expression

   Mammalian

   Mutation

   TadA-CDd mutations; nSpCas9=D10A

   Promoter

   CMV and T7

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-T7-SpCas9-BPNLS(SV40)-3xFLAG-P2A-mScarlet (MNW001)

   Plasmid

   #174091

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a C-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-mScarlet
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV and T7

   Available since

   Sept. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. lenti SYN-SVI-DIO-dCas9-VPR

   Plasmid

   #164576

   Purpose

   Expresses a SYN-driven, intron-containing dCas9-VPR fusion in which the part of the dCas9 cassette is double floxed and in inverted orientation (DIO). Requires Cre-dependent recombination.

   Depositor

   Jeremy Day

   Article

   Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21

   Insert

   A DIO FLAG-dCas9-VPR cassette containing an SV40 intron
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-CBE4max-SpCas9-NG-P2A-EGFP (RTW4554)

   Plasmid

   #140001

   Purpose

   CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized CBE4max SpCas9-NG with P2A-EGFP
   

   Use

   CRISPR

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…

   Promoter

   CAG

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCas9-J23109

    Plasmid

    #113150

    Purpose

    Plasmid constitutively expressing Cas9 protein

    Depositor

    Chong Zhang

    Article

    Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEJS654 All-in-One AAV-sgRNA-hNmeCas9

    Plasmid

    #112139

    Purpose

    Delivery of human-codon-optimized Cas9 from Neisseria meningitidis (NmeCas9) and its single-guide RNA in a single AAV vector for in vivo genome editing.

    Depositor

    Erik Sontheimer

    Article

    Ibraheim et al Genome Biol. 2018 Sep 19;19(1):137. doi:

    Inserts

    sgRNA scaffold
    Human-codon-optimized NmeCas9
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U1a and U6

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-FLEX-SaCas9-U6-sgGria1

    Plasmid

    #124853

    Purpose

    Mutagenesis of Gria1

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gria1 (Gria1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-FLEXfrt-SaCas9-U6-sgGria1

    Plasmid

    #124867

    Purpose

    Mutagenesis of Gria1

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gria1 (Gria1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-FLEX-SaCas9-U6-sgGria3

    Plasmid

    #124855

    Purpose

    Mutagenesis of Gria3

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gria3 (Gria3 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330-NLC-DHFR-SpCas9

    Plasmid

    #124523

    Purpose

    Expresses SpCas9 fused to DHFR domains on both the N- and C- termini and an internal loop in mammalian cells

    Depositor

    Amit Choudhary

    Article

    Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

    Insert

    NLC-DHFR-SpCas9
    

    Use

    AAV

    Tags

    Destabilized domain of E.coli dihydrofolate reduc…

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-FLEXfrt-SaCas9-U6-sgGria3

    Plasmid

    #124869

    Purpose

    Mutagenesis of Gria3

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gria3 (Gria3 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pACYC-T7-SpCas9(BspMI/SapI/BsaI_cassettes)-T7-gRNA1 (BPK1807)

    Plasmid

    #223065

    Purpose

    Dual pT7 entry plasmid for SpCas9(6AA) library; bacterial expression plasmid with type IIS RE cassettes around D1135/S1136, G1218/E1219, R1335/T1337 (precursor to MMW94). Expresses a gRNA.

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    entry vector for human codon opt. bacterial expr. plasmid for SpCas9(6AA_NNS) library, with gRNA targeting EGFP site 1
    

    Use

    CRISPR

    Tags

    NLS(SV40)-3xFLAG

    Expression

    Bacterial

    Mutation

    Three regions of SpCas9 encode type IIS restricti…

    Promoter

    Dual T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pB-CAGGS-dCas9-KRAB

    Plasmid

    #110822

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB

    Depositor

    George Church, Alejandro Chavez

    Article

    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

    Insert

    dCas9-KRAB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CAGGS

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNOC_dCas9-KRAB_sgRNA Td2

    Plasmid

    #176264

    Purpose

    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged to the KRAB domain and Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    dead spCas9
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    Kruppel associated box domain and luciferase

    Expression

    Mutation

    H840A and D10A mutations on spCas9 to inactivate …

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCas9-beta-mmr

    Plasmid

    #169241

    Purpose

    Cas9, beta, and mutL mutant expression plasmid for introducing chromosomal point mutations

    Depositor

    Brian Pfleger

    Article

    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

    Inserts

    cas9
    beta
    mutL-E36K
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Changed glutamic acid 36 to lysine

    Promoter

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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