We narrowed to 9,833 results for: crispr plasmids

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AAV-NG-CBE C

Plasmid

#227422

Purpose

expresses the C-terminal of AAV-NG-CBE

Depositor

Yang Sun

Article

Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

Insert

AAV-NG-CBE C-terminal

Use

AAV and CRISPR

Expression

Mammalian

Promoter

Cbh

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pSimpleII-U6-tracr-U6-crRNA(tdTomato)-NLS-NmCas9-HA-NLS(s)

Plasmid

#47869

Purpose

All-in-one plasmid targeting tdTomato

Depositor

Erik Sontheimer, James Thomson

Article

Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

Inserts

NmCas9
U6pr-tracrRNA
tdTomato crRNA

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Available Since

Sept. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pCas9-beta-mmr

Plasmid

#169241

Purpose

Cas9, beta, and mutL mutant expression plasmid for introducing chromosomal point mutations

Depositor

Brian Pfleger

Article

Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

Inserts

cas9
beta
mutL-E36K

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Mutation

Changed glutamic acid 36 to lysine

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pX330-Flag-dSpCas9

Plasmid

#92113

Purpose

Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

dead/inactive SpCas9 with FLAG tag

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

D10A, H840A

Promoter

Cbh

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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CSAC-Crys

Plasmid

#164967

Purpose

Expresses sgRNA in mammalian cells

Depositor

Han Kyoung Choe

Article

Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

Insert

hU6-sgCry1_2-hU6-sgCry2_1-hU6-sgCry2_2

Use

AAV

Tags

mCherry

Promoter

hU6, hSyn

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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PHY98-LMNA 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)

Plasmid

#164046

Purpose

CRISPR donor plasmid to insert TriTag (mTagBFP) into the N-terminus of human LMNA gene

Depositor

Baohui Chen

Article

Xu et al Nucleic Acids Res. 2020 Oct 26. pii: 594

Insert

mTagBFG-TriTag

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pX330-HA-dSpCas9

Plasmid

#92112

Purpose

Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

dead/inactive SpCas9

Use

CRISPR

Tags

HA tag and NLS

Expression

Mammalian

Mutation

D10A, H840A

Promoter

Cbh

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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CSAC-Bmal1

Plasmid

#168296

Purpose

Expresses sgRNA in mammalian cells

Depositor

Han Kyoung Choe

Article

Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

Insert

hU6-sgBmal1-1-hU6-sgBmal1-3

Use

AAV

Tags

mCherry

Promoter

U6, hSyn

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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HeFm2SpCas9

Plasmid

#92111

Purpose

Expression plasmid for human codon-optimized increased fidelity HeFm2SpCas9 (without U6-sgRNA coding sequence)

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

“Highly enhanced Fidelity” mut2 SpCas9

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

N497A, R661A, Q695A, K848A, Q926A, R1060A

Promoter

Cbh

Available Since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pJT131_GalL_cCDA1-NGBE3

Plasmid

#145095

Purpose

Expressing base editor cCDA1-NGBE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

cCDA1-NGBE3

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…

Promoter

GalL

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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Human T (BRACHYURY) sgRNA

Plasmid

#59726

Purpose

Plasmid encoding sgRNA to generate T (BRACHYURY) knock-out mutant human cells

Depositor

Jacob Hanna

Article

Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

Insert

hT sgRNA (TBXT Human)

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 29, 2014

Availability

Academic Institutions and Nonprofits only
pX330-Flag-wtSpCas9 (without sgRNA)

Plasmid

#92353

Purpose

Expression plasmid for human codon-optimized wild-type SpCas9 (without U6-sgRNA coding sequence)

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

3xFlag-NLS-wild-type SpCas9-NLS

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Promoter

Cbh

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pFB-U6-Chrna7-gRNA-hSyn-mCherry-WPRE-SV40pA

Plasmid

#128342

Purpose

pAAV encoding gRNA sequence for loss-of-function indels

Depositor

Ryan Drenan

Article

Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

Insert

Chrna7 (Chrna7 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFUGW-GFP-2-cut reporter

Plasmid

#131669

Purpose

Lentiviral plasmid that expresses a GFP-based reporter for monitoring CRISPR based precise end-joining.

Depositor

Alberto Ciccia

Article

Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

Insert

GFP-2-cut reporter

Use

Lentiviral

Tags

P2A

Expression

Mammalian

Promoter

EFS-NS

Available Since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pT7-gRNA_zebrafish_mmp21

Plasmid

#72890

Purpose

plasmid to generate guideRNA against mmp21 gene in zebrafish

Depositor

Nicholas Katsanis

Article

Perles et al J Med Genet. 2015 Dec;52(12):840-7. doi:

Insert

zebrafish mmp21 (mmp21 Zebrafish)

Use

CRISPR

Promoter

T7 RNA pol

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pGTPA-lb

Plasmid

#171528

Purpose

Plasmid contains the Cas12k gRNA for mutant library construction

Depositor

Quanjiang Ji

Article

Chen et al Cell Rep. 2021 Aug 31;36(9):109635. doi:

Insert

Cas12k gRNA

Use

CRISPR

Promoter

Constitutive promoter J23119

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pGTPA

Plasmid

#171525

Purpose

Plasmid contains the Cas12k gRNA and transposon donor cassette

Depositor

Quanjiang Ji

Article

Chen et al Cell Rep. 2021 Aug 31;36(9):109635. doi:

Insert

Cas12k gRNA, transposon donor cassette

Use

CRISPR

Promoter

Constitutive promoter J23119

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pMTKM02

Plasmid

#233474

Purpose

SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and split-Nat selection.

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMTKM04

Plasmid

#233476

Purpose

SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and split-G418 selection.

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMTKM06

Plasmid

#233478

Purpose

SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and split-Hyg selection.

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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