We narrowed to 23,366 results for: Nov;

1. BPTF PHD and Bromodomain

   Plasmid

   #92101

   Purpose

   Expresses GST fusion PHD and Bromodomain from BPTF protein

   Depositor

   Albert Jeltsch

   Article

   Mauser et al Biochimie. 2018 Mar;146:14-19. doi: 10.1

   Insert

   PHD and Bromodomain (BPTF Human)
   

   Use

   Tags

   GST Tag

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-MBP-CBX1-eReader

   Plasmid

   #135089

   Purpose

   Bacterial expression of engineered CBX1 with enhanced binding to H3K9me

   Depositor

   Marcey Waters

   Article

   Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

   Insert

   CBX1 chromobox protein 1, engineered
   

   Use

   Tags

   6xhis-tag and MBP

   Expression

   Bacterial

   Mutation

   lysine 43 mutated to alanine; aspartic acid 59 mu…

   Promoter

   T7

   Available since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTS110 pHAGE2 CMVtetO2 MCS-22xGS-Alfa-P2A-TagBFP

   Plasmid

   #199363

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally ALFA-P2A-BFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   ALFA-P2A-TagBFP

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTS97 pHAGE2 CMVtetO2 GFP-22xGS-SUMOstar-MCS

   Plasmid

   #199350

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOstar-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP-SUMOstar

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. hSirt3-RFP

   Plasmid

   #216806

   Purpose

   Human Sirt3-RFP driven by the CaMKII promoter

   Depositor

   Ghazaleh Ashrafi

   Article

   Tiwari et al J Cell Biol. 2024 Jan 1;223(1):e20230504

   Insert

   Sirt3 (SIRT3 Human)
   

   Use

   Lentiviral

   Tags

   mRFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CamKII

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMarVF1

   Plasmid

   #99579

   Purpose

   Minitransposon delivery vector pMarVF1 (suicide vector) in V. fischeri ES114

   Depositor

   Mark Mandel

   Article

   Brooks JF et al Proc Natl Acad Sci U S A. 2014 Dec 2;111

   Insert

   V. fischeri mariner transposon (with erythromycin-resistance (ErmR) cassette)
   

   Use

   Suicide vector in v. fischeri es114

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pThermeleon

   Plasmid

   #86120

   Purpose

   Bandpass temperature-gated expression of mWasabi and mCherry reporters

   Depositor

   Mikhail Shapiro

   Article

   Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

   Inserts

   TlpA
   mWasabi
   TcI38
   mCherry
   cI
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A67T, M1V, L65S, K68R, F115L, D126G, D188G and D3…

   Promoter

   pR/pL, pR/pL, pLacI, and pTlpA

   Available since

   March 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-p38beta

   Plasmid

   #86833

   Purpose

   mammalian expression of GFP-p38beta

   Depositor

   Rony Seger

   Article

   Zehorai et al Mol Cell Biol. 2014 Jan;34(2):259-70. do

   Insert

   p38beta (MAPK11 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQmod2C-GG

   Plasmid

   #191347

   Purpose

   Clostridium expression vector (pBP1 origin, cmR) with drop-out Plac-RFP cassette flanked with BsaI sites

   Depositor

   Andrew Tolonen

   Article

   Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

   Insert

   Plac-RFP (IPTG-inducible red fluorescent protein)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Plac

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEFIRES-P-mTagBFP-KDEL

    Plasmid

    #87163

    Purpose

    Fluorescent marker of endoplasmic reticulum (ER)

    Depositor

    Elina Ikonen

    Article

    Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

    Insert

    mTagBFP-KDEL
    

    Use

    Tags

    Bip signal peptide (Homo sapiens) and mTagBFP-KDE…

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1 alpha

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. AF1521-c003

    Plasmid

    #173111

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    AF_1521 (AF_RS07675 Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126))
    

    Use

    Tags

    Nanoluc

    Expression

    Bacterial

    Mutation

    KH-M1-L192

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5-H2B-AID-EYFP

    Plasmid

    #47329

    Purpose

    for plasmid integration using the Flp-In system. AID at C-terminal

    Depositor

    Don Cleveland

    Article

    Holland et al Proc Natl Acad Sci U S A. 2012 Dec 4;109

    Insert

    histone H2B (H2BC21 Human)
    

    Use

    Tags

    AID and EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFX-mito-eqFP650

    Plasmid

    #174179

    Purpose

    Express eqFP650 with mitochondria-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    eqFP650 with mitochondria-targeting sequence
    

    Use

    Tags

    the mitochondria-targeting sequence of the cytoch…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTS106 pHAGE2 CMVtetO2 MCS-3C-SUMOEu-22xGS-GFP

    Plasmid

    #199359

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOEu-GFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-SUMOEu-EGFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. GFP-JNK2

    Plasmid

    #86831

    Purpose

    mammalian expression of GFP-JNK2

    Depositor

    Rony Seger

    Article

    Zehorai et al Mol Cell Biol. 2014 Jan;34(2):259-70. do

    Insert

    JNK2 (MAPK9 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMXS-IRES-Blast coUGP2

    Plasmid

    #211531

    Purpose

    Expresses codon optimized UGP2

    Depositor

    Richard Possemato

    Article

    Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

    Insert

    UGP2 (UGP2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAcGFP1-mito

    Plasmid

    #174180

    Purpose

    Express AcGFP1 with mitochondria-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    AcGFP with mitochondria-targeting sequence
    

    Use

    Tags

    the mitochondria-targeting sequence of the cytoch…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMT-ADARcd-E488Q-V5

    Plasmid

    #139686

    Purpose

    Expresses only the hyperactive form of the ADAR catalytic domain - no RNA binding protein. This is a control plasmid to be used with pMT-Hrp48-ADARcd-E488Q-V5

    Depositor

    Michael Rosbash

    Article

    Xu et al RNA. 2018 Feb;24(2):173-182. doi: 10.126

    Insert

    Adenosine deaminase acting on RNA (Adar Fly)
    

    Use

    Tags

    V5

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. Teto BRN3A

    Plasmid

    #62221

    Purpose

    human BRN3A cDNA

    Depositor

    Kristin Baldwin

    Article

    Blanchard et al Nat Neurosci. 2015 Jan;18(1):25-35. doi:

    Insert

    BRN3A (POU4F1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    deleted a.a. 142-181

    Promoter

    Available since

    April 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFX-EGFP-Sec61β

    Plasmid

    #174466

    Purpose

    Express EGFP with endoplasmic reticulum-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    Sirius with endoplasmic reticulum-targeting sequence
    

    Use

    Tags

    Sec61 subunit beta

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pBOB-CARMIL2 BH domain-GFP

    Plasmid

    #118736

    Purpose

    To express CARMIL2 BH tagged with GFP on C-terminus

    Depositor

    John Cooper

    Article

    Lanier et al J Biol Chem. 2016 Jan 15;291(3):1076-91.

    Insert

    CARMIL2 BH domain (CARMIL2 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTS98 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-MCS

    Plasmid

    #199351

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TagBFP-P2A-ALFA

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pATmin-GG

    Plasmid

    #191352

    Purpose

    Clostridium expression vector (pAMB1 origin, ermR) with drop-out Plac-RFP cassette flanked with BsaI sites

    Depositor

    Andrew Tolonen

    Article

    Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

    Insert

    Plac-RFP (IPTG-inducible red fluorescent protein)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. APLF-c001

    Plasmid

    #173077

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    APLF (APLF Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    K360-V448, codon optimized

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. FlagMBNL1-41

    Plasmid

    #96906

    Purpose

    expresses Flag tagged human MBNL1-41 kDa isoform from pcDNA3.1 HisC

    Depositor

    Thomas Cooper

    Article

    Grammatikakis et al Nucleic Acids Res. 2011 Apr;39(7):2769-8

    Insert

    MBNL1-41 kDa isoform (MBNL1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAC-BETA-At

    Plasmid

    #53288

    Purpose

    Contains crtE, crtB, and crtI genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and an lcyB cDNA of Arabidopsis thaliana. Use with pY2F to produce alpha-carotene in E. coli.

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Eukaryot Cell. 2007 Mar;6(3):533-45. Epu

    Insert

    lcyB (LYC Mustard Weed)
    

    Use

    Low copy number bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pQdC12a

    Plasmid

    #191356

    Purpose

    Clostridium CRISPRi plasmid with TetR-repressible dCas12a (LbCpf1) expression

    Depositor

    Andrew Tolonen

    Article

    Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

    Inserts

    PGusA2-TetO2/1-dCas12a (LbCpf1)
    miniPthl-tetR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PGus2A-TetO2/1 and miniPthl

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMT-HuC:Dendra2

    Plasmid

    #80904

    Purpose

    Injection into Zebrafish larvae together with Tol2-Transposase to achieve pan-neuronal expression of the photoconvertible fluorescent protein Dendra2

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Nat Protoc. 2016 Dec;11(12):2419-2431. d

    Insert

    Dendra2 (elavl3 Synthetic)
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    HuC

    Available since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1-GNPTAB

    Plasmid

    #78108

    Purpose

    Mammalian expression of human GNPTAB with C terminal myc tag

    Depositor

    Thomas Braulke

    Article

    Franke et al J Biol Chem. 2013 Jan 11;288(2):1238-49.

    Insert

    GNPTAB (GNPTAB Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 19, 2016

    Availability

    Academic Institutions and Nonprofits only

30. BPK1520_puroR

    Plasmid

    #173901

    Purpose

    SpCas9 guide cloning vector derived from BPK1520 that allows for puromycin-based selection

    Depositor

    Ronald Cohn

    Article

    Erwood et al Genome Res. 2019 Dec;29(12):2010-2019. d

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/U6

    Available since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge