We narrowed to 19,050 results for: Tec

1. AA299

   Plasmid

   #216029

   Purpose

   Fragmid fragment: (Cas protein) NNGG PAM

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Cas9_v1.1 [Slug]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. AA296

   Plasmid

   #216028

   Purpose

   Fragmid fragment: (Cas protein) NNNNCC PAM

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Nme2Cas9_v1.1 [Nme2]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AA180

   Plasmid

   #216023

   Purpose

   Fragmid fragment: (Cas protein) NGRR(T) PAM

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Cas9_v1.1 [Sa]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AA301

   Plasmid

   #216030

   Purpose

   Fragmid fragment: (Cas protein) NNGG PAM

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Cas9_v1.1 [Sauri]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AA027

   Plasmid

   #216007

   Purpose

   Fragmid fragment: (Cas protein) nearly PAM-less

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Cas9-SpRY_v1.1 [Sp]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. AA026

   Plasmid

   #216006

   Purpose

   Fragmid fragment: (Cas protein) NG PAM

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Cas9-SpG_v1.1 [Sp]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTrc99S-ssDsbA-EPA-DNNNS-DQNRT

   Plasmid

   #128404

   Purpose

   Periplasmic expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites in E. coli

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Insert

   Exotoxin A (toxA P. aeruginosa)
   

   Tags

   6xHis tag and DsbA signal sequence for periplasmi…

   Expression

   Bacterial

   Mutation

   242DNNNS246 and 384DQNRT388 engineered glycosylat…

   Promoter

   trc

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEDF-PhdRS

   Plasmid

   #127445

   Purpose

   Double mutant of wild type mmPylRS designed for incorporation of non-cannonical amino acids in to M13 bacteriophage

   Depositor

   Wenshe Liu

   Article

   Tharp et al Nat Commun. 2020 Mar 13;11(1):1392. doi:

   Insert

   Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
   

   Expression

   Bacterial

   Mutation

   N346A-C348A mutations on PylRS

   Promoter

   araBAD

   Available Since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-dCpf1-BE-YE

   Plasmid

   #107686

   Purpose

   Expresses dLbCpf1-BE-YE in mammalian cells

   Depositor

   Jia Chen

   Article

   Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   dLbCpf1-BE-YE
   

   Expression

   Mammalian

   Mutation

   APOBEC1(W90Y/R126E), dLbCpf1(D832A/E925A/D1148A)

   Promoter

   CMV

   Available Since

   April 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDR348 FANCF

    Plasmid

    #47510

    Purpose

    human gRNA expression vector targeting FANCF

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-FANCF (FANCF Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNAS1b split mCherry 14-3-3β ctrl

    Plasmid

    #112031

    Purpose

    Mode #2 phosphosite control (split mCherry, using 14-3-3β binding domain paired with phospho-dependent interacting peptide with TAG codon)

    Depositor

    Jesse Rinehart

    Article

    Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

    Insert

    14-3-3β fused to C-terminal split mCherry, phospho-dependent binding ctrl peptide with TAG fused to N-terminal split mCherry
    

    Tags

    Split mCherry

    Expression

    Bacterial

    Available Since

    July 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-dSaCas9-KRAB-blast

    Plasmid

    #191654

    Purpose

    Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain for gene silencing and the blasticidin resistance gene.

    Depositor

    Moshe Szyf

    Article

    Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

    Insert

    dSaCas9-KRAB
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-(pos36)GFP-9xGGS-Cre-6xHis

    Plasmid

    #62938

    Purpose

    Expression of (+36)GFP-GGS9-Cre-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    (ps36)GFP-9xGGS-Cre
    

    Tags

    (pos36)GFP and 6xHis tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. Ctcf-Halo-mAID donor

    Plasmid

    #113103

    Purpose

    Donor plasmid for knock-in mAID-Halo fusion to the c-terminal of mouse Ctcf coding sequence

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    c terminal Ctcf donor region including homology arms and mAID-HaloTag (Ctcf Mouse, Synthetic)
    

    Use

    Synthetic Biology

    Available Since

    Aug. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOCC119

    Plasmid

    #118884

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal monomeric GFP, cleavable with TEV

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-mGFP-stop-HindIII cassette
    

    Tags

    HIS6-MBP, cleavable with 3C protease and mGFP, cl…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTrc99S-ssDsbA-TTc-4xDQNAT

    Plasmid

    #128401

    Purpose

    Periplasmic expression of tetanus toxin fragment C with C terminal 4xDQNAT glycosylation sites in E. coli

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Tetanus toxin fragment C domain
    

    Tags

    4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

    Expression

    Bacterial

    Promoter

    trc

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTrc99S-ssDsbA-TTlight-4xDQNAT

    Plasmid

    #128402

    Purpose

    Periplasmic expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites in E. coli

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Tetanus toxin light chain domain
    

    Tags

    4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

    Expression

    Bacterial

    Mutation

    Inactivating E234A mutation

    Promoter

    trc

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. mCerulean-LaminB1-10

    Plasmid

    #55380

    Purpose

    Localization: Nuclear Envelope, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    LaminB1 (LMNB1 Human)
    

    Tags

    mCerulean

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pCellFree_G03 BATF3

    Plasmid

    #67050

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    BATF3 (BATF3 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

20. EK0445 SFFV-mCherry-SGc(DNAJB1.s1)cSG-EGFP-(3xMS2) (FLP-IN)

    Plasmid

    #191155

    Purpose

    Expression of a sensor for human DNAJB1 (hsp40) 3' UTR in mammalian cells; mCherry marker, EGFP output, three MS2 sequences in 3' UTR

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:DNAJB1.s1:EGFP-3xMS2
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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