We narrowed to 14,728 results for: EGF

1. pCMV-T7-ABE8e-SpCas9(LWQYQH)-P2A-EGFP (AHK504)

   Plasmid

   #223119

   Purpose

   pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with LWQYQH amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized TadA8e-SpCas9(LWQYQH)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-P2A-EGFP and BPNLS-TadA8e

   Expression

   Mammalian

   Mutation

   TadA8e mutations; nSpCas9(D10A/LWQYQH)=D1135L, S1…

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-T7-ABE8e-SpCas9(MWKYQS)-P2A-EGFP (AHK505)

   Plasmid

   #223120

   Purpose

   pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized TadA8e-SpCas9(MWKYQS)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-P2A-EGFP and BPNLS-TadA8e

   Expression

   Mammalian

   Mutation

   TadA8e mutations; nSpCas9(D10A/MWKYQS)=D1135M, S1…

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-T7-ABE8e-SpCas9(MWKYQA)-P2A-EGFP (AHK503)

   Plasmid

   #223122

   Purpose

   pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MWKYQA amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized TadA8e-SpCas9(MWKYQA)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-P2A-EGFP and BPNLS-TadA8e

   Expression

   Mammalian

   Mutation

   TadA8e mutations; nSpCas9(D10A/MWKYQA)=D1135M, S1…

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-T7-ABE8e-SpCas9(KWRQLC)-P2A-EGFP (RAS3467)

   Plasmid

   #223112

   Purpose

   pCMV and pT7 Human expression plasmid for ABE8e SpCas9 enzyme with KWRQLC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized TadA8e-SpCas9(KWRQLC)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-P2A-EGFP and BPNLS-TadA8e

   Expression

   Mammalian

   Mutation

   TadA8e mutations; nSpCas9(D10A/KWRQLC)=D1135K, S1…

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-T7-ABE8e-SpCas9(MRKCRS)-P2A-EGFP (BKS1034)

   Plasmid

   #223117

   Purpose

   pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MRKCRS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized TadA8e-SpCas9(MRKCRS)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-P2A-EGFP and BPNLS-TadA8e

   Expression

   Mammalian

   Mutation

   TadA8e mutations; nSpCas9(D10A/MRKCRS)=D1135M, S1…

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSynI-EGFP-WPRE-hGH poly(A) (Alex_02)

   Plasmid

   #71651

   Purpose

   AAV expression of EGFP in human and mouse neurons

   Depositor

   Mirjana Maletić-Savatić

   Insert

   EGFP
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. KL158 - pBlueScript II SK-T2A-eGFP-SV40pA Podxl donor

   Plasmid

   #232936

   Purpose

   Donor plasmid for knock-in of T2A-eGFP-SV40pA at the mouse Podxl locus. Used with PX459-sgRNA047_Podxl sgRNA.

   Depositor

   Danny Reinberg

   Article

   Lucero et al bioRxiv, March 19, 2025

   Insert

   T2A-eGFP-SV40pA with Podxl homology arms
   

   Use

   Mouse Targeting

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX4T1_GST-EGFP-ATG13_IDR_191-517aa_(M196-H214 11xA)

   Plasmid

   #223779

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   ATG13 IDR (ATG13 Human)
   

   Tags

   GST-EGFP

   Expression

   Bacterial

   Mutation

   expresses aa 191-517, with M196A S197A R199A G202…

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX4T1_GST-EGFP-ATG13_IDR_delta206-230 (191-205_231-517aa)

   Plasmid

   #223787

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   ATG13 IDR (ATG13 Human)
   

   Tags

   GST-EGFP

   Expression

   Bacterial

   Mutation

   expresses aa 191-517, with 206-230 deleted

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE-FKBP-mEGFP-WIPI2 IDR (364-425aa)

    Plasmid

    #223758

    Purpose

    Stable expression and inducible recruitment of protein in mammalian cell culture

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    WIPI2 IDR (WIPI2 Human)
    

    Use

    Lentiviral

    Tags

    FKBP-mEGFP

    Mutation

    aa 364-425 only

    Available Since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pETDuet1_6xHis-TEV-EGFP-FIP200-CTR(1429-1591aa)

    Plasmid

    #223724

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    FIP200-CTR (RB1CC1 Human)
    

    Tags

    6xHis-TEV-EGFP

    Expression

    Bacterial

    Mutation

    aa 1429-1591 only

    Available Since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV.U6-sasgRNA(SapI)_Ple155-CI-EGFP-W3SL_BbsI(GGA)

    Plasmid

    #231367

    Purpose

    Ple155-driven EGFP, also encoding U6-driven sgRNA cassette, with SapI sites to clone crRNA sequence

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    EGFP
    

    Use

    AAV

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. Const.3 EGFP-P2A-dTomato-T2A-TagBFP-PS9

    Plasmid

    #231907

    Purpose

    For packaging EGFP, dTomato and TagBFP mRNA into SARS-CoV-2 virus-like particles (VLPs)

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Insert

    EGFP-P2A-dTomato-T2A-TagBFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. Const.5 EGFP-P2A-dTomato-IRES-Luc-PS9

    Plasmid

    #231909

    Purpose

    For packaging EGFP, dTomato and firefly luciferase mRNA into SARS-CoV-2 virus-like particles (VLPs)

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Insert

    EGFP-P2A-dTomato-IRES-Luc
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pME nls EGFP WPRE bGH pA (JDW 488)

    Plasmid

    #224540

    Purpose

    A Gateway compatible middle entry clone containing a 3x-NLS-EGFP for labeling the nucleus

    Depositor

    Joshua Wythe

    Article

    Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

    Insert

    nls-EGFP-WPRE-bGH-pA
    

    Use

    Gateway cloning

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO-puro SNAP-CD86-eGFP-FKBP(f36v)

    Plasmid

    #200283

    Purpose

    Membrane protein fusion with extracellular SNAPtag and intracellular FKBP(f36v) dimerization domain and GFP reporter under Tetracycline-inducible promoter

    Depositor

    Kristina Ganzinger

    Article

    Niederauer et al Nat Commun. 2023 Jul 19;14(1):4345. doi:

    Insert

    SNAPtag-eGFP-CD86tm-eGFP-FKBP(f36v)
    

    Use

    Lentiviral

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-hSyn-FLEX-macaque OPN4-IRES-EGFP

    Plasmid

    #223253

    Purpose

    AAV construct for Cre-dependent (FLEX) expression of macaque melanopsin (Opn4, short isoform, GenBank:OP856641; hSyn promoter) and of EGFP via an IRES.

    Depositor

    Michael Do

    Article

    Liu et al Science. 2023 Jan 27;379(6630):376-381.

    Insert

    macaque OPN4-IRES-EGFP (OPN4 )
    

    Use

    AAV

    Promoter

    human Synapsin 1 (hSyn)

    Available Since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX552-EF1a-DIO Chronos-eGFP(with gRNA scaffold)

    Plasmid

    #199582

    Purpose

    Expresses Chronos-eGFP in a Cre-dependent manner

    Depositor

    Bryan Copits

    Article

    Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

    Insert

    Chronos eGFP
    

    Use

    AAV, CRISPR, and Cre/Lox

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    EF1a intron A

    Available Since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDGB3_alpha1_LIR1-35S-eGFP-T35S-SIR-LIR2 (GB4312)

    Plasmid

    #225435

    Purpose

    BeYDV LIR1 + TU 35S:eGFP:T35S + BeYDV SIR-LIR2.

    Depositor

    Diego Orzaez

    Article

    Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

    Insert

    LIR1:35S:eGFP:T35S-SIR:LIR2
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHIV-NAT-T2A-hCD52-eGFP-NFATC2IP-SPR

    Plasmid

    #221447

    Purpose

    Lentiviral mammalian expression vector of eGFP-tagged NFATC2IP ORF that is fused with S-phase restricted (SPR) degron at its C-terminus

    Depositor

    Daniel Durocher

    Article

    Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

    Insert

    NFATC2IP (NFATC2IP Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    SPR degron: hGEM (1-110) fragment that is fused t…

    Promoter

    CMV

    Available Since

    July 3, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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