We narrowed to 18,503 results for: REV

1. pLenti-Blast-sgNT

   Plasmid

   #138678

   Purpose

   Expresses a Non-targeting sgRNA and Cas9

   Depositor

   Reuben Shaw

   Article

   Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

   Insert

   sgNT
   

   Use

   Lentiviral

   Promoter

   hU6

   Available Since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. MASTL B4.4 gRNA

   Plasmid

   #90755

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human MASTL

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   MASTL (Guide Designation B4.4)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. LZF1735 pAAV_hSyn-DiO-somArchon1-EGFP-P2A-somCheRiff

   Plasmid

   #126512

   Purpose

   Optopatch4. Simultaneous optical recording and manipulation of electrical activity in neurons

   Depositor

   Adam Cohen

   Article

   Fan et al Cell. 2020 Jan 23. pii: S0092-8674(20)30

   Insert

   somArchon1-EGFP-P2A-somCheRiff
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. LITE2.0 EF1a_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_hGHpA

   Plasmid

   #47457

   Purpose

   LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. EF1-alpha promoter for ubiquitous mammalian expression.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

   Insert

   NLS(alpha-imp)-CRY2PHR-NLS-VP64
   

   Tags

   2A_GFP

   Expression

   Mammalian

   Promoter

   EF1-alpha

   Available Since

   Oct. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. AMPK-β2-FLAG

   Plasmid

   #40603

   Depositor

   Harvey Lodish

   Article

   Wong et al J Biol Chem. 2006 Nov 24;281(47):36434-4

   Insert

   AMPK-β2 (Prkab2, Prkab2 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pCA528 AMSH 1-178

   Plasmid

   #160050

   Purpose

   Expresses MIT domain (residues 1-178) of AMSH as a HIS-SUMO fusion protein. Internal ID: WISP18-22.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   AMSH residues 1-178 (STAMBP Human)
   

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 1-178

   Promoter

   T7

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. NDUFV3_pcDNA6.2/EmGFP-Bsd

   Plasmid

   #176946

   Purpose

   Mammalian expression vector encoding NDUFV3 and EmGFP-Bsd

   Depositor

   Roger Reeves

   Article

   Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

   Insert

   NDUFV3 (NDUFV3 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. CFP-CD82

   Plasmid

   #1818

   Depositor

   Walther Mothes

   Article

   Sherer et al Traffic 2003 Nov;4(11):785-801.

   Insert

   CD82 (CD82 Human)
   

   Tags

   CFP

   Expression

   Mammalian

   Available Since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. Sema3c(L)-AP-His

   Plasmid

   #72015

   Purpose

   Expresses the Sema3C protein (truncated at cleavage site P3; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sema3c (Sema3c Mouse)
   

   Tags

   AP-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. p21 shRNA2

    Plasmid

    #25869

    Depositor

    Sally Temple

    Article

    Fasano et al Cell Stem Cell. 2007 Jun 7. 1(1):87-99.

    Insert

    p21 (Cdkn1a Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTV-Sox2_5prime-1000-24MS96T-3F_NeoR

    Plasmid

    #174884

    Purpose

    Targeting vector for generating Sox2 STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    STREAMING-tag with homology arms for Sox2
    

    Use

    Mouse Targeting

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOPINK-OTUD1 (OTU+UIM, aa 287-481)

    Plasmid

    #61406

    Purpose

    Expresses human OTUD1 (OTU+UIM domains) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD1 (OTUD1 Human)
    

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-286.

    Promoter

    T7

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCLXSN(GFP)-myc-BioID-hp100

    Plasmid

    #174738

    Purpose

    Gene overexpression

    Depositor

    Shao-Cong Sun

    Article

    Li et al Cell Res. 2019 Jun;29(6):474-485. doi: 1

    Insert

    P100 (NFKB2 Human)
    

    Use

    Retroviral

    Mutation

    WT

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. GB1-ALIX(P801G)

    Plasmid

    #180025

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(1-868, P801G). P801G mutation facilitates expression in E. coli.

    Depositor

    Lalit Deshmukh

    Article

    Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

    Insert

    GB1-His6-TEV-ALIX(1-868, P801G) (PDCD6IP Human)
    

    Tags

    GB1-His6-TEV

    Expression

    Bacterial

    Mutation

    P801G

    Available Since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-GG-hUBC-EBFP2

    Plasmid

    #212314

    Purpose

    Lentiviral backbone with EBFP2 reporter

    Depositor

    Andrew Wilson

    Article

    Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5/FLAG-HA-DGCR8

    Plasmid

    #106251

    Purpose

    Express DGCR8 in mammalian cells

    Depositor

    Joshua Mendell

    Article

    Chang et al Genome Res. 2015 Sep;25(9):1401-9. doi:

    Insert

    DiGeorge Syndrome Critical Region 8 (DGCR8 Human)
    

    Tags

    FLAG-HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAG32-3HA-3FLAG

    Plasmid

    #154081

    Purpose

    For C-terminal 3xHA-3xFLAG-protein tagging in S. pombe using hphMX4 as resistance marker.

    Depositor

    Cornelia Kilchert

    Article

    Kilchert et al Genome Res. 2020 Jul;30(7):1012-1026. do

    Insert

    3xHA-3xFLAG-hygR
    

    Use

    Pcr-based tagging in yeast

    Tags

    3xHA-3xFLAG

    Available Since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pST1374-GCN4-D10A

    Plasmid

    #113022

    Purpose

    Expresses GCN4-D10A in mammalian cells

    Depositor

    Xingxu Huang

    Article

    Jiang et al Cell Res. 2018 Jun 6. pii: 10.1038/s4142

    Insert

    GCN4-D10A (GCN4 Budding Yeast, S. pyogenes)
    

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CMV

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHAGE-FLAG-APEX2-LGALS1

    Plasmid

    #175755

    Purpose

    Lentiviral vector expressing N-terminally tagged APEX2-FLAG-LGALS1

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    LGALS1 (LGALS1 Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAV10.KH

    Plasmid

    #63208

    Purpose

    pCACS backbone (Amp), Hyg, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. pEGFP-C1-BBS5

    Plasmid

    #218714

    Purpose

    Expresses N-terminally EGFP-tagged BBS5 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

    Insert

    BBS5 (BBS5 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. TfR-mCh-DHFR

    Plasmid

    #173013

    Purpose

    For light regulated release of transferrin receptor (TfR) from the endoplasmic reticulum. Driven by CMV promoter.

    Depositor

    Matthew Kennedy

    Article

    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    TfR-mCh-DHFR
    

    Tags

    DHFR and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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23. pJS288

    Plasmid

    #200714

    Purpose

    To express yeast Hxt1 hexose transporter in yeast

    Depositor

    Jens Boch

    Article

    Fuhrmeister et al Bio Protoc. 2023 Aug 5;13(15):e4733. doi

    Inserts

    PMA1 promoter (PMA1 Budding Yeast)
    Hxt1 (HXT1 Budding Yeast)
    ADH2 Ter (ADH2 Budding Yeast)
    

    Use

    Synthetic Biology

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    domesticated for MoClo cloning (removal of intern…

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. pcDNA5FRT/TO-UBXD7-Strep-HA

    Plasmid

    #113479

    Purpose

    Expression of human UBXD7 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    UBXD7 (UBXN7 Human)
    

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    A834C silent mutation, wildtype without stop codon

    Promoter

    CMV

    Available Since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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25. pHAGE-FLAG-APEX2-CALCOCO2

    Plasmid

    #175759

    Purpose

    Lentiviral vector expressing N-terminally tagged APEX2-FLAG-CALCOCO2/NDP52

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    NDP52/CALCOCO2 (CALCOCO2 Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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26. KIF20A D5.2 gRNA

    Plasmid

    #90718

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human KIF20A

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    KIF20A (Guide Designation D5.2)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. p663-UBC-miniTurbo-V5-NRF3

    Plasmid

    #207815

    Purpose

    N-terminal tagged NRF3 for miniTurbo proximity experiments

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    miniTurbo-V5-NRF3 (NFE2L3 Human)
    

    Use

    Lentiviral

    Tags

    miniTurbo-V5

    Promoter

    UBC

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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28. p667-UBC-NRF3-V5-miniTurbo

    Plasmid

    #207816

    Purpose

    C-terminal tagged NRF3 for miniTurbo proximity experiments

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    NRF3-V5-miniTurbo (NFE2L3 Human)
    

    Use

    Lentiviral

    Tags

    V5-miniTurbo

    Promoter

    UBC

    Available Since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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29. pJR021

    Plasmid

    #198814

    Purpose

    Variant of yellow fluorescent protein for transient gene expression

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::destabilizedYFP::let-838 3'UTR
    

    Expression

    Worm

    Mutation

    YFP with destabilized signal from mouse ornithine…

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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30. Plxnb1(L)-Fc-His

    Plasmid

    #72126

    Purpose

    Expresses the entire extracellular region of the PlexinB1 protein (ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Plxnb1 (Plxnb1 Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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