We narrowed to 27,002 results for: Vit

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pAAV-hSynapsin1-axon-GCaMP6m

Plasmid

#111261

Purpose

For enriched expression of GCaMP6m in axons

Depositor

Lin Tian

Article

Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

Has Service

AAV9

Insert

axon-GCaMP6m

Use

AAV

Tags

GAP43 palmitoylation domain

Expression

Mammalian

Promoter

hSynapsin1

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pRRL-5xTetO-pEF-H2B-Citrine-SV40

Plasmid

#186966

Purpose

Lentiviral vector encoding fluorescent reporter (5xTetO-pEF-H2B-Citrine-SV40) for expression in mammalian cells.

Depositor

Brian Liau

Article

Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d

Insert

5xTetO-pEF-H2B-Citrine-SV40

Use

Lentiviral

Expression

Mammalian

Promoter

5xTetO-pEF-1alpha

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mCherry-Rheb(5A)

Plasmid

#192439

Purpose

Encodes mCherry-tagged Rheb lacking residues 38-42.

Depositor

Jin Zhang

Article

Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

Insert

mCherry-Rheb(C181S) (RHEB Human)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Rheb amino acids 38-42 deleted.

Promoter

CMV

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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AiP12068 - pAAV-DLX2.0-minBG-ChrimsonR-tdTomato-WPRE3-BGHpA (Alias: CN2068)

Plasmid

#229775

Purpose

enhancer AAV for expression of ChrimsonR-tdTomato under the DLX2.0 enhancer

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Matveev et al bioRxiv [Preprint]. 2024 Nov 1:2024.11.0

Insert

ChrimsonR-tdTomato

Use

AAV

Tags

tdTomato

Expression

Mammalian

Available Since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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mcherry-Myo 9b R1695M

Plasmid

#134958

Purpose

Expression of rat Myo 9b (Myr 5) in mammalian cells.GAP minus mutant.

Depositor

Martin Bähler

Article

Hemkemeyer et al J Biol Chem. 2021 Jan-Jun;296:100136. do

Insert

Myo 9b (rat) with 3' UTR. GAP minus mutant R1695M (Myo9b Rat)

Tags

mcherry

Expression

Mammalian

Promoter

CMV IE

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AktAR-Kras

Plasmid

#123357

Purpose

FRET-based biosensor for monitoring Akt activity in non-raft plasma membrane regions.

Depositor

Jin Zhang

Article

Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

Insert

AktAR-Kras

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-BE4-SsAPOBEC3B R54Q

Plasmid

#138344

Purpose

Mammalian expression plasmid for BE4-SsAPOBEC3B R54Q

Depositor

Nicole Gaudelli

Article

Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

Insert

BE4-SsAPOBEC3B R54Q

Use

Synthetic Biology

Tags

BP-NLS

Promoter

CMV

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1-sh-mSlug-3

Plasmid

#40647

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

sh-mSlug-3 (Snai2 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
pJSK418

Plasmid

#205131

Purpose

Expression of GFP-2xFYVE in Dictyostelium

Depositor

Jason King

Article

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

2xFYVE (from human Hrs) (HGS Synthetic)

Use

Dictyostelium expression

Tags

EGFP

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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Flag Nprl2 pLJM1

Plasmid

#46338

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Nprl2 (NPRL2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only
lentiCRISPRv2-TTF-1-gRNA

Plasmid

#101857

Purpose

Introducing a gRNA targeting human TTF-1 (NKX2-1) into cells

Depositor

David Mu

Article

Lai et al Oncogene. 2018 Mar 19. pii: 10.1038/s413

Insert

gRNA targeting TTF-1 (NKX2-1 Human)

Use

Lentiviral

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-Myo 1d

Plasmid

#134906

Purpose

Expression of rat Myo 1d (Myr 4) in mammalian cells.

Depositor

Martin Bähler

Article

Bahler et al J Cell Biol. 1994 Jul;126(2):375-89. doi

Insert

Myo 1d (rat) with 3' UTR (Myo1d Rat)

Tags

EGFP

Expression

Mammalian

Promoter

CMV IE

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV_tKiMBI-T2A-caMEK

Plasmid

#199578

Purpose

Expresses tKiMBI and caMEK in an AAV vector

Depositor

Michael Lin

Article

Wu et al ACS Cent Sci. 2023 Mar 20;9(4):719-732.

Inserts

ERK tdTomato-Kinase-Modulated Bioluminescent Indicator
constitutively active MEK

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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SG3ΔENV CA E28A,E29A

Plasmid

#145785

Purpose

Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and E28A,E29A mutation in capsid (CA E28A,E29A).

Depositor

Wesley Sundquist

Article

Christensen et al Science. 2020 Oct 9;370(6513). pii: 370/

Insert

Capsid

Use

Lentiviral and Retroviral

Expression

Mammalian

Mutation

Changed Capsid residues Glutamic Acid residues 28…

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_R1079A

Plasmid

#83494

Purpose

Bacterial expression of L. buccalis C2c2 CRISPR effector (R1079A- pre-crRNA processing mutant)

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_R1079A

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

R1079A

Promoter

T7

Available Since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pCDH-CIBN-CAAX

Plasmid

#188989

Purpose

A lentiviral plasmid encoding plasma membrane-tagged CIBN

Depositor

Jeremy Baskin

Article

Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

Insert

CIBN (CIB1 Mustard Weed)

Tags

CAAX

Expression

Mammalian

Promoter

CMV

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe-PI(WT)-Src(YF)-mCherry

Plasmid

#87357

Purpose

PI(WT): Photo-inhibitable/blue light sensitive; YF: Y535F const. active mutation

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
Src isoform 2 (Src Mouse)

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

Y535F

Promoter

CMV

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-BE4-RrA3F F130L

Plasmid

#138341

Purpose

Mammalian expression plasmid for BE4-RrA3F F130L

Depositor

Nicole Gaudelli

Article

Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

Insert

BE4-RrA3F F130L

Use

Synthetic Biology

Tags

BP-NLS

Promoter

CMV

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-H2B-mRuby3-IRES-eGtACR1-ST

Plasmid

#108960

Purpose

Anion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites under the control of internal ribosome entry sequence

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

eGtACR1-ST

Expression

Mammalian

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pFLAG-ING5

Plasmid

#13292

Depositor

Curtis Harris

Article

Shiseki et al Cancer Res. 2003 May 15. 63(10):2373-8.

Insert

ING5 (ING5 Human)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only