We narrowed to 18,503 results for: REV

1. pLV-Lck-FRB-mTurquoise2

   Plasmid

   #176130

   Purpose

   Plasma membrane localized FRB, rapamycin heterodimerization

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   FRB
   

   Use

   Lentiviral

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. DKC1 F1.3 gRNA

   Plasmid

   #90652

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human DKC1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   DKC1 (Guide Designation F1.3)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   July 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. Cytosolic Fyn FRET Biosensor

   Plasmid

   #124654

   Purpose

   FRET biosensor. The highly sensitive biosensor has a mutated SH2 domain, ECFP and YPet

   Depositor

   Yingxiao Wang

   Article

   Ouyang et al ACS Sens. 2019 Jan 25;4(1):76-86. doi: 1

   Insert

   Synthetic gene containing ECFP-SH2 domain-substrate-linker-YPet
   

   Expression

   Mammalian

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAD16_CAD_12-490_pvp008

   Plasmid

   #124842

   Purpose

   Expresses Aspergillus terreus CAD in E.coli

   Depositor

   Konrad Buessow

   Article

   Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

   Insert

   cis-aconitate decarboxylase
   

   Tags

   StrepTag and TEV site

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-11, synthetic codon optimiz…

   Promoter

   T7

   Available Since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRK5-HA-YFP-DEPTOR

   Plasmid

   #73390

   Purpose

   Expresses HA and YFP tagged DEPTOR

   Depositor

   Jie Chen, Taekjip Ha

   Article

   Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

   Insert

   DEP Domain Containing MTOR-Interacting Protein (DEPTOR Human)
   

   Tags

   HA and YFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

6. ALIX(1-702)-mNeonGreen

   Plasmid

   #191193

   Purpose

   Truncated form of ALIX that lacks C-terminal proline-rich domain; C-terminally tagged with mNeonGreen.

   Depositor

   Jin Zhang

   Article

   Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

   Insert

   ALIX (1-702) (PDCD6IP Human, Synthetic)
   

   Tags

   6xHIS and mNeonGreen

   Expression

   Mammalian

   Mutation

   Deletion of ALIX C-terminal PRD.

   Promoter

   CMV

   Available Since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVX-mCherry-SPASTIN

   Plasmid

   #180647

   Purpose

   Lentiviral expression vector for SPASTIN. Used for generating cell lines. Has N-terminal mCherry tag. SPASTIN starts on M87. Dox-inducible. Internal ID: WISP20-43.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   SPASTIN
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Starts at M87, has siRNA resistance to GAACAGUGUG…

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-CamKIIα-CRY2PHR-P2A-CIBN-mCherry-VAMP2

   Plasmid

   #178574

   Purpose

   Lentiviral vector for CamKIIα::Opto-vTrap

   Depositor

   Won Do Heo

   Article

   Won et al Neuron. 2022 Feb 2;110(3):423-435.e4. do

   Insert

   CRY2PHR-P2A-CIBN-mCherry-VAMP2
   

   Use

   Lentiviral

   Promoter

   CaMKIIa

   Available Since

   April 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR SFFVp MCP-mCherry IRES h2b-diRFP

   Plasmid

   #102351

   Purpose

   Lentiral expression vector bearing the MS2 Coat Protein (MCP) fused to mCherry as well as the nuclear marker histone 2b fused to two copies of irFP. Also bears the hygromycin selection marker.

   Depositor

   Jared Toettcher

   Article

   Wilson et al Mol Cell. 2017 Sep 7;67(5):757-769.e5. d

   Insert

   MS2 Coat Protein
   

   Use

   Lentiviral

   Tags

   mCherry

   Promoter

   SFFV

   Available Since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. DKC1 F2.3 gRNA

    Plasmid

    #90653

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human DKC1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    DKC1 (Guide Designation F2.3)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBBK84 pHomL-RPL18Bp-PDR16-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

    Plasmid

    #179068

    Purpose

    Vector containing a yeast integration cassette for RPL18B-driven expression of PDR16-Link-Scarlet(rfp), a marker for the lipid droplet

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    RPL18Bp-PDR16-Link-Scarlet(rfp)
    

    Expression

    Yeast

    Available Since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBBK79 pHomL-RPL18Bp-Scarlet(rfp)-Link-YPT7-SSA1t-HomR (AmpR)

    Plasmid

    #179063

    Purpose

    Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-YPT7, a marker for the vacuole, late endosome

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    RPL18Bp-Scarlet(rfp)-Link-YPT7
    

    Expression

    Yeast

    Available Since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET21-GFP-AURKA

    Plasmid

    #99875

    Purpose

    Expression of GFP-AURKA for protein purification in bacteria

    Depositor

    Marc Tramier

    Article

    Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Tags

    GFP

    Expression

    Bacterial

    Available Since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. AE-Polyubiquitin-GCaMP6s

    Plasmid

    #106868

    Purpose

    Expresses polyubiquitin GCaMP6s, a genetically encoded calcium indicator, for live calcium imaging in Aedes aegypti mosquitoes.

    Depositor

    Omar Akbari

    Article

    Bui et al BMC Neurosci. 2019 Jun 17;20(1):27. doi:

    Insert

    AE-Polyubiquitin-GCAaMP6s
    

    Expression

    Insect

    Promoter

    Ae. aegypti polyubiquitin (PUb)

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV7.1_3xFLAG-TAOK3

    Plasmid

    #172993

    Purpose

    constitutive expression of FLAG-tagged TAOK3

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    3xFLAG-TAOK3 (TAOK3 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5FRT/TO-p37-Strep-HA

    Plasmid

    #113485

    Purpose

    Expression of human p37 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    p37 (UBXN2B Human)
    

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available Since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. FEN1 C10.3 gRNA

    Plasmid

    #90689

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human FEN1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    FEN1 (Guide Designation C10.3)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. Sema3e(L)-AP-His

    Plasmid

    #72019

    Purpose

    Expresses the Sema3E protein (truncated at cleavage site P3; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema3e (Sema3e Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. GB1-PRD(800-868)

    Plasmid

    #141345

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(800-868).

    Depositor

    Lalit Deshmukh

    Article

    Elias et al Proc Natl Acad Sci U S A. 2020 Sep 11. p

    Insert

    GB1His6-TEV-ALIX(800-868)
    

    Tags

    GB1-His6-TEV

    Expression

    Bacterial

    Available Since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHH0103_SKAP2-1/1

    Plasmid

    #91447

    Purpose

    Protein expression and purification of human SH3 domain construct SKAP2-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SKAP2-1/1 (SKAP2 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. mCherry-NES-SspB-C3Gcat-P2A-iLIDcaax

    Plasmid

    #173874

    Purpose

    Mammalian expression of mVenus-NES-SspB-C3Gcat-P2A-iLIDcaax (opto-Rap)

    Depositor

    Takao Nakata

    Article

    Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

    Insert

    mCherry-NES-SspB-C3Gcat-P2A-iLIDcaax
    

    Tags

    C-terminal CAAX-box and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. Sema4g-Fc-His

    Plasmid

    #72160

    Purpose

    Expresses the extracellular region of the Sema4G protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema4g (Sema4g Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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23. cpGFP-Adhiron_flex

    Plasmid

    #165044

    Purpose

    Flexible linker control for POLArISact expression in mammalian cells

    Depositor

    Sumio Terada

    Article

    Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11

    Insert

    cpGFP-Adhiron (connected with a flexible linker)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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24. pAAV-Syn-FLEX-ExSYTE

    Plasmid

    #216263

    Purpose

    Cre-dependent expressoin of ExSYTE (P2A) EGFP with 3'UTR of Rat Arc DTE under synapsin promoter

    Depositor

    Susumu Tomita

    Article

    Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

    Insert

    ExSYTE P2A EGFP - Rat DTE
    

    Use

    AAV

    Available Since

    May 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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25. SMC6 G4.4 gRNA

    Plasmid

    #90899

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SMC6

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    SMC6 (Guide Designation G4.4)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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26. SMC6 G3.4 gRNA

    Plasmid

    #90898

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SMC6

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    SMC6 (Guide Designation G3.4)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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27. pSaPIPLC His

    Plasmid

    #173810

    Purpose

    Expression and purification of mature, His-tagged Staphylococcus aureus (strain FPR3757) phosphaatidyl inositol specific phospholipase C (PI-PLC) from E. coli BL21 CodonPlus (DE3) RIL cells

    Depositor

    Anne Gershenson, Mary F Roberts

    Article

    Goldstein et al Biochemistry. 2012 Mar 27;51(12):2579-87

    Insert

    Staphylococcus aureus plc
    

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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28. Sema3g.a(L)-Fc-His

    Plasmid

    #72151

    Purpose

    Expresses the Sema3G, isoform a protein (truncated at cleavage site P3; ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema3g.a (Sema3g Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. AAEL006511-Cas9

    Plasmid

    #100580

    Purpose

    The promoter of AAEL006511 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

    Inserts

    AAEL006511-hspcas9-GFP
    Opie2-dsRed
    

    Use

    CRISPR

    Tags

    GFP and dsRED

    Expression

    Insect

    Promoter

    AAEL006511 and Opie2

    Available Since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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30. pBBK80 pHomL-RPL18Bp-Scarlet(rfp)-Link-VAM3-SSA1t-HomR (AmpR)

    Plasmid

    #179064

    Purpose

    Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-VAM3, a marker for the vacuole

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    RPL18Bp-Scarlet(rfp)-Link-VAM3
    

    Expression

    Yeast

    Available Since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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