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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,065 results for: Tet-on

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Showing: 7341 - 7360 of 10065 results

  1. pHBintT

    Plasmid
    #48134
    Purpose
    Shuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker tetracycline for B. megaterium, integration vector
    Depositor
    Dieter Jahn
    Article
    Biedendieck et al Microb Biotechnol. 2010 Jan;3(1):24-37.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Oct. 11, 2013
    Availability
    Academic Institutions and Nonprofits only
    48134-plasmid-map-sequence-id-69938

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  2. pSS9

    Plasmid
    #71655
    Purpose
    Contains 600 bp homology arms to SS9 for one-step markerless genome integration in E. coli
    Depositor
    Ryan Gill
    Article
    Bassalo et al ACS Synth Biol. 2016 Apr 22.
    Insert
    gfpuv
    Use
    Synthetic Biology
    Promoter
    T7A1
    Available Since
    Jan. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    71655-plasmid-map-sequence-id-257561

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  5. pMM178

    Plasmid
    #61270
    Purpose
    Also referred to as pRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains a pBBR1 origin and chloramphenicol resistance.
    Depositor
    Timothy Lu
    Article
    Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103
    Insert
    Cas9, tracrRNA, crRNA
    Use
    CRISPR
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    pLtetO, native, native (respectively)
    Available Since
    April 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    61270-plasmid-map-sequence-id-99577

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  6. p1D4-hrGFP II

    Plasmid
    #71317
    Purpose
    expression vector with 1D4 epitope added as a C-terminal tag, allowing for in vitro expression of any visual pigment or GPCR of interest.
    Depositor
    Belinda Chang
    Article
    Morrow et al Plasmid. 2010 Nov;64(3):162-9. doi: 10.1
    Type
    Empty backbone
    Tags
    1D4 epitope (TETSQVAPA) and IRES-hrGFPII
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  16. pJO629

    Plasmid
    #104342
    Purpose
    Express yeast Yap5-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.
    Depositor
    Alexander Varshavsky
    Article
    Oh et al J Biol Chem. 2017 Dec 29;292(52):21457-2
    Insert
    pTDH3tc3-Yap5-3xFLAG-tCyc1-pTDHtc3-flagDHFRha-tCyc1 (YAP5 Budding Yeast)
    Tags
    3xFLAG
    Expression
    Yeast
    Available Since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    104342-plasmid-map-sequence-id-207903

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Showing: 7341 - 7360 of 10065 results