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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 25,284 results for: crispr

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Showing: 7361 - 7380 of 25284 results

  5. enDelIscB

    Plasmid
    #247329
    Purpose
    Vector encoding human codon-optimized engineered DelIscB driven by CAG promoter, optimized sgRNA (sgRNA-V5) driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yong Tian
    Insert
    pCAG_enDelIscB_npNLS_polyA_pU6_sgRNA_V5-BsaI_pCMV_mCherry
    Expression
    Mammalian
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    247329-plasmid-map-sequence-id-496503

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  7. eTd-CBEm

    Plasmid
    #196602
    Purpose
    Mammalian expression of eTd-CBEm
    Depositor
    Dali Li
    Publication
    Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    bpNLS-TadA-8e (N46L/A48M)-nSpCas9-bpNLS-P2A-UGI-bpNLS
    Tags
    6*His
    Expression
    Mammalian
    Available since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    196602-plasmid-map-sequence-id-386797

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  9. pMEDS

    Plasmid
    #236568
    Purpose
    Introduces a multiple expression and dual selection (MEDS) system to use alongside CRISPR for applications such as gene editing.
    Depositor
    Shannon Moonah
    Publication
    Sharma et al PLoS One. 2025 May 6;20(5):e0321881. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    CD/UPRT (negative selection)
    NPT II (positive selection)
    Tags
    3x Myc and FLAG
    Expression
    Mammalian
    Available since
    June 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    236568-plasmid-map-sequence-id-477092

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  14. pTUB1-Spy.dCas9-CAT-U6-sgRNA(decoy)

    Plasmid
    #171089
    Purpose
    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus pyogenes in Toxoplasma gondii. Suitable for generating stable cell lines.
    Depositor
    Sebastian Lourido
    Publication
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Inserts
    Decoy sgRNA
    dCas9
    Chloramphenicol acetyltransferase
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3X FLAG and Nuclear Localization Signal
    Mutation
    D10A, H840A
    Promoter
    TUB1 (Toxoplasma gondii) and U6 (Toxoplasma gondi…
    Available since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171089-plasmid-map-sequence-id-339962

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Showing: 7361 - 7380 of 25284 results