We narrowed to 4,304 results for: GFP reporter gene
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Plasmid#185855PurposeTesting the SkGAL2 promoter inserted with 2 PZ4 elements using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2M1(2*PZ4)> (BamHI)yEGFP>TURA3
ExpressionYeastMutationNoneAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP8EFA
Plasmid#185854PurposeTesting a heat inducible protein degradation tag -DHFR P66L using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1>UBI4>RHGSGTMV>DHFRP66L>yEGFP>TPGK1-TURA3
ExpressionYeastMutationDHFR P66LAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3A2 (1)
Plasmid#185851PurposeTesting the TEF1 promoter appended with 2 tetracycline riboswitch using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1>2*TcRb>yEGFP>TPGK1-TURA3
ExpressionYeastMutationNoneAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDual-eGFP
Plasmid#63215PurposeExpression of eGFP in bacteria and in mammalian cells. Used as a reporter for quantifying gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP
UseLentiviralTagsHis6 and T7ExpressionBacterial and MammalianPromoterCMV-EF1α hybrid (CEF)Available SinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
SHC003BSD-DelGFP
Plasmid#133301PurposeEGFP reporter was removed and the selection marker was modified as blasticidin in the construct of SHC003. This construct could be used for expression of a gene by lentiviral approach.DepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceOct. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQE30-roGFP1-iE
Plasmid#83312PurposeInducible expression of redox sensitive GFP in bacteriaDepositorInsertroGFP1-iE
Tags6xHISExpressionBacterialMutationro1(C48S/S147C/Q204C) plus X insertion, iE betwe…PromoterT5Available SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-FeGFP
Plasmid#138261PurposeDonor-eGFP vector to be used in plasmid to plasmid integration reporter assay. eGFP is conditionally expressed when integrated at iCas9-sites on acceptor plasmidDepositorInserteGFP
UseCRISPRExpressionMammalianPromoterNoneAvailable SinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
7Gli:GFP
Plasmid#110494Purposereporter of Hedgehog signaling activityDepositorInsert7 repeats of the Gli binding site GAACACCCA
UseLentiviralExpressionMammalianPromoter7 repeats of Gli biding siteAvailable SinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2]
Plasmid#115893PurposeAAV-mediated expression of Archon1-KGC-GFP-ER2 under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.DepositorHas ServiceAAV8InsertArchon1-KGC-EGFP-ER2
UseAAVTagsER2, GFP, and KGCExpressionMammalianPromoterSynAvailable SinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α1.1-FLEX-rc [Archon1-KGC-GFP-ER2]
Plasmid#115891PurposeAAV-mediated expression of Archon1-KGC-GFP-ER2 under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.DepositorInsertArchon1-KGC-GFP-ER2
UseAAVTagsER2, GFP, and KGCExpressionMammalianPromoterEF1α promoter (1.1kb short version)Available SinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1202 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 4xΦ]
Plasmid#107845PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
ExpressionMammalianMutationAll hydrophobics combined in four clusters in spl…Available SinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHBS1203 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 2xΦ]
Plasmid#107844PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
ExpressionMammalianMutationAll hydrophobics combined in two clusters in spli…Available SinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHBS1201 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 6xΦ]
Plasmid#107846PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
ExpressionMammalianMutationAll hydrophobics combined in six clusters in spli…Available SinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3b-8XGliBS:EGFP
Plasmid#84602PurposeGli protein-dependent EGFP reporter for analysis of Hedgehog signalingDepositorInserteGFP
ExpressionMammalianPromoter8X Gli-binding siteAvailable SinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
LV-Sox10MCS5-GFP
Plasmid#115783PurposeThis plasmid encodes for Sox10-MCS5-GFP reporter. Cell carrying this construct expresses green fluorescence under the control of SOX-MCS5 enhancer conjugated with cfos basal promoter.DepositorInsertmouse derived SOX10 Multiple Species Conserved enhancer element 5 conjugated with cfos basal promoter
UseLentiviralTagscfos basal promoter conjugated MCS5 promoter fuse…PromoterSOX10 Multiple Species Conserved enhancer element…Available SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1333 (IBB-GFP-mCherry 3E splicer)-(BFP-P2A-TDP43 G368W W385G)
Plasmid#107855PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
ExpressionMammalianMutationG368W and W385G in splicing reporterAvailable SinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
Tol2-U6.3-sgRNA-DNM1L -GFP
Plasmid#221845PurposeTol2 transposon expressing sgRNA targeting chick DNM1L from chick U6.3 promoter expresses GFP reporter from GAGC promoterDepositorInsertsEGFP
DNM1L sgRNA-gTGTTTTCCGACCATCCTCTG
UseCRISPR; Tol2 transposon optimised for chick expre…ExpressionMammalianPromoterCAGC and U6.3 chickAvailable SinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tol2-U6.3-sgRNA-MFN1-GFP
Plasmid#221846PurposeTol2 transposon expressing sgRNA targeting chick MFN1 from chick U6.3 promoter expresses GFP reporter from GAGC promoteDepositorInsertsEGFP
MFN1 sgRNA-GAGAAGAAGAGCGTCAAGGT
UseCRISPR; Tol2 transposon optimised for chick expre…ExpressionMammalianPromoterCAGC and U6.3 chickAvailable SinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
CAG-loxP-3xpA-loxP-td-sfGFP
Plasmid#180150Purposetd-sfGFP cre reporterDepositorInserttd-sfGFP
UseCre/LoxExpressionMammalianAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
-5.5kb-UCP1-GFP
Plasmid#104585PurposeReporter assay to monitor the transcription activity of the UCP1 promoter by GFP expressionDepositorAvailable SinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
Arch(D95H)-eGFP
Plasmid#51081PurposeExpresses Arch(D95H)-eGFP in mammalian cells as a fluorescent reporter of membrane voltageDepositorInsertArchaerhodopsin-3 w/ D95H point mutation fused to eGFP
UseLentiviralTagseGFPExpressionMammalianMutationChanged Aspartic Acid 95 to HistidinePromoterUbiquitinAvailable SinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10-VEGFprom(-1000 to -1)
Plasmid#66128PurposeLuciferase-based reporter for VEGF promoter region (-1000 to -1 bp)DepositorInsertVascular Endothelial Growth Factor -A promoter region (VEGFA Human)
UseLuciferasePromoterVEGF -1000 to -1Available SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT2/LTR7-GFP
Plasmid#62541PurposeLTR7-driving reporterDepositorInsertLTR7-GFP
UseSleeping beauty transposonExpressionMammalianPromoterLTR7Available SinceJan. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMVS102_P3-GFP-NATMX6
Plasmid#99048PurposeEGFP reporter with six binding sites for the Zif268 DNA binding domainDepositorInsertsMcIsaac 2014 P3 promoter
eGFP
clonNAT resistance
ExpressionYeastMutationGAL1 promoter with 4 GAL4 sites removed and repla…PromoterMcIsaac 2014 P3 promoterAvailable SinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
TNF-SBP-EGFP_puromycin
Plasmid#65284PurposeTNF reporter only (no hook) (RUSH system)DepositorInsertTNF (TNF Human)
TagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianPromoterCMVAvailable SinceJuly 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10-VEGFprom(-500 to -1)
Plasmid#66130PurposeLuciferase-based reporter for VEGF promoter region (-500 to -1 bp)DepositorInsertVascular Endothelial Growth Factor-A promoter region (VEGFA Human)
UseLuciferasePromoterVEGF -500 to -1Available SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_3E5E_eGFP
Plasmid#103054PurposeEncodes Ebola virus minigenome with EGFP reporter gene. Minigenome is under control of the CAG promoterDepositorInsertEbola virus (ebolavirus Zaire, Mayinga) minigenome
Tags3E5E eGFP cloned into pCAGGS vector from pCDNA3.1…ExpressionMammalianPromoterCAGAvailable SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
mE-GFP2A
Plasmid#236247PurposeMiddle entry fluorescent GFP reporter with a viral 2A peptide for use with the Gateway cloning system; allows 3' transgene to be transcribed on the same mRNA but translated separatelyDepositorInserteGFP
ExpressionMammalianMutationAdded a viral2A sequence with no stop codonAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLKO‐puro‐2A‐mdx4cv‐GFP
Plasmid#187053Purposefluorescent reporter for adenine base editing of mdx4cv point mutationDepositorInsertGFP
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3E-APEX2-P2A-EGFP
Plasmid#108896PurposeMultisite gateway vector for 3' tagging with APEX2 (includes cytoplasmic GFP reporter)DepositorInsertAPEX2-P2A-EGFP
UseMultisite gatewayAvailable SinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
EGFP-P2A-APEX2-ChBP
Plasmid#108879PurposeExpression of APEX2 fused to a nanobody against mCherry (includes green cytoplasmic reporter)DepositorInsertEGFP-P2A-APEX2-ChBP
ExpressionMammalianAvailable SinceNov. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
EGFP-P2A-APEX2-csChBP
Plasmid#108877PurposeExpression of APEX2 fused to a conditionally stable nanobody against mCherry (includes green cytoplasmic reporter)DepositorInsertEGFP-P2A-APEX2-csChBP
ExpressionMammalianAvailable SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME-EGFP-P2A-APEX2-NS
Plasmid#108895PurposeMultisite gateway vector for 5' tagging with APEX2 (includes cytoplasmic GFP reporter)DepositorInsertEGFP-P2A-APEX2
UseMultisite gatewayAvailable SinceMay 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
E17TAG GFP zeo resistance
Plasmid#69118PurposeFor E17TAG GFP reporter expression in non-zeocin resistant strainsDepositorInsertE17TAG GFP
TagsHisExpressionBacterialMutationE17 to TAGAvailable SinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAW-GFP-2xmiR-34 (pAW-AS-25)
Plasmid#40833PurposemiRNA sensor used in Drosophila cell linesDepositorInsertTwo target sites perfectly complementary to miR-34
UseMirna reporterTagsEGFPExpressionInsectPromoterActin5CAvailable SinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAW-EGFP-2xmiR-1 (pAW-AS-21)
Plasmid#40832PurposemiRNA sensor used in Drosophila cell linesDepositorInsertTwo target sites perfectly complementary to miR-1
UseMirna reporterTagsEGFPExpressionInsectPromoterActin5CAvailable SinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLoxP-EGFP-crRNA-entry
Plasmid#213048PurposeSWITCHER ready LoxP-EGFP reporter plasmid for cloning of a customized CRISPR-inducible Cre-constructDepositorInsertLoxP-EGFP-MALAT1-triplex
UseCRISPRExpressionMammalianAvailable SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGFP-AUA-IRES-mCherry
Plasmid#222116PurposeStart codon reporter with mutated start codonDepositorInsertGFP-IRES-mCherry
UseLentiviralMutationAUA start codonAvailable SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGFP-AGC-IRES-mCherry
Plasmid#222119PurposeStart codon reporter with mutated start codonDepositorInsertGFP-IRES-mCherry
UseLentiviralMutationAGC start codonAvailable SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGFP-CUG-IRES-mCherry
Plasmid#222110PurposeStart codon reporter with mutated start codonDepositorInsertGFP-IRES-mCherry
UseLentiviralMutationCUG start codonAvailable SinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only