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pCS-H2B-cerulean

Plasmid

#53748

Purpose

Contains a fusion of human histone H2B to cerulean fluorescent protein in the pCS2+ vector.

Depositor

Sean Megason

Publication

Megason et al Methods Mol Biol. 2009;546:317-32. doi:

Insert

histone H2B (H2BC21 Human)

Tags

Cerulean

Expression

Mammalian

Promoter

simian CMV IE94

Available since

July 8, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV-hSyn-ASAP3Kv-WPRE

Plasmid

#132332

Purpose

expresses ASAP3Kv(soma-located version of ASAP3, ASAP-family genetically encoded voltage indicator) in mammalian cells, gene delivered by pAAV-hSyn vector

Depositor

Michael Lin

Publication

Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

Insert

ASAP3Kv

Use

AAV

Expression

Mammalian

Promoter

hSynapsin

Available since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBad-mAmetrine

Plasmid

#18083

Purpose

Bacterial expression of mAmetrine fluorescent protein

Depositor

Robert Campbell

Publication

Ai et al Nat Methods. 2008 May;5(5):401-3. doi: 1

Insert

mAmetrine fluorescent proteins

Tags

His

Expression

Bacterial

Mutation

The fluorescent protein was inserted between XhoI…

Available since

May 29, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-3xFlag

Plasmid

#132403

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H133A; H1059A

Available since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCAGGS-Akt-FoxO3a-KTR-EGFP

Plasmid

#125131

Purpose

Fluorescent reporter for Akt activity

Depositor

Kazuhiro Aoki

Publication

Maryu et al Cell Struct Funct. 2016 Jul 22;41(2):81-

Insert

FOXO2 (FOXO3 Human)

Tags

Enhanced green fluorescent protein (EGFP)

Expression

Mammalian

Mutation

FoxO3A 1-402 amino acid

Promoter

CAG promoter

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pRcCMV myc-Plk1 T210D (Nigg LA183)

Plasmid

#41158

Depositor

Erich Nigg

Publication

Smits et al Nat Cell Biol. 2000 Sep;2(9):672-6.

Insert

Plk1 (PLK1 Human)

Tags

Myc

Expression

Mammalian

Mutation

T210D

Promoter

CMV

Available since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pAAV-hSyn1-FLEX-NEPLDCV-P2A-mRUBY3-WPRE

Plasmid

#207664

Purpose

Neuropeptide degradation

Depositor

Sung Han

Publication

Kim et al bioRxiv. 2023 Jan 20:2023.01.19.524797.

Insert

NEP (MME Human)

Use

AAV and Cre/Lox

Tags

mRUBY3

Mutation

Addition of signaling peptide (POMC) on the N-ter…

Promoter

hSynapsin

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-RAB11B-IRES-Puromycin

Plasmid

#134860

Purpose

Expresses EGFP-tagged recycling endosome marker

Depositor

David Andrews

Publication

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

RAB11B (RAB11B Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLV_Hygro_mini-mAgrin-Myc-His

Plasmid

#198130

Purpose

Express mini-Agrin in mammalian cells

Depositor

Jeffrey Miner

Insert

mini-Agrin (Agrn Mouse)

Use

Lentiviral

Tags

TEV-Myc-His

Promoter

EF1a

Available since

June 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti CMV TRE3G Neo GFP-Lamin A wildtype

Plasmid

#118709

Purpose

Lentiviral TET-ON inducible GFP-Lamin A wildtype

Depositor

Tom Misteli

Publication

Kubben et al Methods. 2015 Sep 1. pii: S1046-2023(15)

Insert

GFP-Lamin A human wildtype (LMNA Human)

Use

Lentiviral; Tet-on inducible

Tags

GFP

Expression

Mammalian

Promoter

CMV TRE3G (TET-ON)

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pmCherry-C1 actin-3XNLS P2A mCherry

Plasmid

#58475

Purpose

Expresses nuclear-targeted human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter

Depositor

Dyche Mullins

Publication

Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

Inserts

beta actin (ACTB Human)
3XNLS
P2A cleavage site

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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IF311: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-LSD1

Plasmid

#121824

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the LSD1 H3K4me1/2 demethylase for 3 or 4-component MultiSite Gateway Pro assembly.

Depositor

Christopher Newgard

Publication

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Sa-dCas9/LSD1 (open)

Use

Gateway Cloning and Synthetic Biology

Tags

NLS

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTCB-magroHCLALAPGknob6xHis

Plasmid

#237375

Purpose

pTwist CMV BetaGlobin encoding magrolimab heavy chain with LALA P329G knob Fc and 6xHis tag

Depositor

Carolyn Bertozzi, Jessica Stark

Publication

Stark et al Nat Biotechnol. 2025 Dec 16. doi: 10.103

Insert

magrolimab heavy chain (silent Fc)

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTCB-magroLC

Plasmid

#237376

Purpose

pTwist CMV BetaGlobin encoding magrolimab light chain

Depositor

Carolyn Bertozzi, Jessica Stark

Publication

Stark et al Nat Biotechnol. 2025 Dec 16. doi: 10.103

Insert

magrolimab light chain

Expression

Mammalian

Promoter

CMV

Available since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR221-MAGI2(v1)

Plasmid

#166182

Purpose

Gateway entry vector for N. furzeri MAGI2

Depositor

Daniel Jarosz

Publication

Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

Insert

MAGI2(v1)

Use

Gateway Cloning

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-MAGI2(v2)

Plasmid

#166183

Purpose

Gateway entry vector for N. furzeri MAGI2

Depositor

Daniel Jarosz

Publication

Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

Insert

MAGI2(v2)

Use

Gateway Cloning

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-MAGT1_STOP

Plasmid

#161468

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

MAGT1 (MAGT1 Human)

Expression

Mammalian

Available since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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VP-MAGE-2-429

Plasmid

#89125

Purpose

mammalian expression of full-length human MAGE-A11 with N-terminal VP16 activation domain

Depositor

Elizabeth Wilson

Publication

Bai et al Mol Cell Biol. 2005 Feb;25(4):1238-57.

Insert

MAGE-A11 (MAGEA11 Human)

Tags

VP16

Expression

Mammalian

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only
4X3C mAGNET

Plasmid

#67954

Purpose

Lentiviral vector coding EGFP followed by 4x miR-128, 4x miR-221 and 4x miR-222 target sequences

Depositor

Xue Han, Wilson Wong

Publication

Sayeg et al ACS Synth Biol. 2015 Apr 13.

Type

Empty backbone

Use

Lentiviral

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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4X2C mAGNET

Plasmid

#67953

Purpose

Lentiviral vector coding EGFP followed by 4x miR-128 and 4x miR-221 target sequences

Depositor

Xue Han, Wilson Wong

Publication

Sayeg et al ACS Synth Biol. 2015 Apr 13.

Type

Empty backbone

Use

Lentiviral

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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