We narrowed to 1,166 results for: ncl

1. pJH751

   Plasmid

   #172171

   Purpose

   Microscopy inclusion body assay

   Depositor

   Matthew Bennett

   Article

   Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

   Insert

   pCDF.JM10.1, deleted sumo
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Evolved PylBCD operon; SUMO tag deleted from PylB…

   Promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. KG#471

   Plasmid

   #110935

   Purpose

   Expresses proteins in a subset of neurons in C. elegans, including isolated expression in the DA9 cholinergic motor neuron in the tail

   Depositor

   Kenneth Miller

   Article

   Edwards et al Genetics. 2015 Sep;201(1):91-116. doi: 1

   Inserts

   mig-13 promoter
   unc-54 3' control region
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLY133

   Plasmid

   #130961

   Purpose

   Engineered sgRNA-LEB4 generator including Anderson promoter J23100 for golden gate assembly

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

   Insert

   sgRNA-LEB4
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Anderson promoter J23100

   Available since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLY86

   Plasmid

   #130949

   Purpose

   Engineered sgRNA-LEA2-WTmis2 generator circuit including promoter Plux2, 2 BoxB aptamers and 2 mis-matches in sgRNA scaffold

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

   Insert

   sgRNA-LEA2-WTmis2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Plux2

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLY132

   Plasmid

   #130960

   Purpose

   Engineered sgRNA-LEB3 generator including Anderson promoter J23100 for golden gate assembly

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

   Insert

   sgRNA-LEB3
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Anderson promoter J23100

   Available since

   Sept. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLY130

   Plasmid

   #130952

   Purpose

   Engineered sgRNA-LEB1 generator including Anderson promoter J23100 for golden gate assembly

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

   Insert

   sgRNA-LEB1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Anderson promoter J23100

   Available since

   Sept. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. R2Ct (1-520)

   Plasmid

   #115649

   Purpose

   expression of truncated Rag2 including the C-terminal region

   Depositor

   Martin Gellert

   Article

   Grundy et al Proc Natl Acad Sci U S A. 2010 Dec 28;10

   Insert

   Rag2 (1-520) (Rag2 Mouse)
   

   Use

   Tags

   8XHis-MBP-PreScission

   Expression

   Mammalian

   Mutation

   truncated Rag2 (aa 1-520)

   Promoter

   Available since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pES1004

   Plasmid

   #21790

   Depositor

   Richard Yu

   Article

   Yu et al Nature. 2008 Dec 11. 456(7223):755-61.

   Insert

   Kss1-as2-trunc (KSS1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   deleted bases 1-14 from open reading frame E94A …

   Promoter

   Available since

   Oct. 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p075 pCS xTRPS1 delN delC119 (M)

   Plasmid

   #11045

   Depositor

   Ramesh Shivdasani

   Article

   Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

   Insert

   TRPS1 delN delC119 mut (trps1 Frog)
   

   Use

   Xenopus, mammalian, avian, and zebrafish

   Tags

   Expression

   Mutation

   aa806-1153 of xTRPS1. Two Cys residues in the GA…

   Promoter

   Available since

   Dec. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

10. p076 pCS xTRPS1 delN delC320 (M)

    Plasmid

    #11046

    Depositor

    Ramesh Shivdasani

    Article

    Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

    Insert

    TRPS1 (trps1 Frog)
    

    Use

    Mammalian, avian, and zebrafish

    Tags

    Expression

    Mutation

    aa806-952 of xTRPS1. Two Cys residues in the GAT…

    Promoter

    Available since

    Dec. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

11. p063 pCS xTRPS1 delta N (M)

    Plasmid

    #11036

    Depositor

    Ramesh Shivdasani

    Article

    Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

    Insert

    TRPS1 deltaN mut (trps1 Frog)
    

    Use

    Mammalian, avian, and zebrafish

    Tags

    Expression

    Mutation

    aa806-1153 of xTRPS1. Two Cys residues in the GA…

    Promoter

    Available since

    Dec. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

12. pR26 CAG AsiSI/MluI

    Plasmid

    #74286

    Purpose

    Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter and loxP flanked stop cassette, for cloning into AsiSI or Mlui

    Depositor

    Ralf Kuehn

    Article

    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

    Insert

    Rosa26 5-homology region
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAGIG_V5-PPP6C

    Plasmid

    #92013

    Purpose

    Expresses V5-PPP6C, includes IRES-Emerald

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    PPP6C (PPP6C Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLX303-ZIM3-KRAB-dCas9

    Plasmid

    #154472

    Purpose

    Expresses ZIM3 KRAB domain fused to the N terminus of dCas9

    Depositor

    Mikko Taipale

    Article

    Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

    Insert

    ZIM3 (ZIM3 Synthetic)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Includes ZIM3 aa 1-100

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTS2355_Tier3(PB)-iRFP_PuroR

    Plasmid

    #169654

    Purpose

    Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a iRFP670 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-iRFP670-p2A-PuroR-pA-3'ITR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PRPBSA-driven iRFP670 and PuroR expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PRPBSA

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPagP

    Plasmid

    #112125

    Purpose

    Bacterial expression of outer membrane protein PagP, wild type

    Depositor

    Karen Fleming

    Article

    Burgess et al J Biol Chem. 2008 Sep 26;283(39):26748-5

    Insert

    PagP (pagP E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N-terminal signal sequence not included and repla…

    Promoter

    T7

    Available since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pR26 CAG/GFP Dest

    Plasmid

    #74281

    Purpose

    Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, GFP, for gateway cloning

    Depositor

    Ralf Kuehn

    Article

    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

    Insert

    Rosa26 5-homology region
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCRISPRa_all-in-one

    Plasmid

    #183695

    Purpose

    Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective

    Depositor

    Edward Avezov

    Article

    Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pR26 CAG/GFP Asc

    Plasmid

    #74285

    Purpose

    Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette and GFP, for cloning into AscI

    Depositor

    Ralf Kuehn

    Article

    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

    Insert

    Rosa26 5-homology region
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. trPAGmEos3.2

    Plasmid

    #203752

    Purpose

    Encodes the transmembrane domain from PAG/CSK, including its 2 palmitoylation sites, with mEos3.2 fused on the C terminus. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    trPAG (PAG1 Human)
    

    Use

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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