We narrowed to 1,214 results for: poli

1. pBz12

   Plasmid

   #226321

   Purpose

   E. coli protein expression for protein purification

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Insert

   6xHis-Rubisco
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLz92

   Plasmid

   #226317

   Purpose

   E. coli protein expression for protein purification

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Insert

   6xHis-CsoS2B
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTU1

   Plasmid

   #72934

   Purpose

   Backbone vector with internal BsaI removed by site-directed mutagenesis

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBP

   Plasmid

   #72947

   Purpose

   Backbone vector with internal BsmBI removed by site-directed mutagenesis

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCellFree_G05 sfGFP

   Plasmid

   #67045

   Purpose

   Cell-free expression control

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   sfGFP
   

   Use

   Cell-free expression

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

6. p5lysAsfGFP

   Plasmid

   #92025

   Purpose

   sfGFP reporter plasmid with lysA TALE binding site in its promoter

   Depositor

   Brian Pfleger

   Article

   Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

   Insert

   sfGFP
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p5sucAmCherry

   Plasmid

   #92026

   Purpose

   mCherry reporter plasmid with sucA TALE binding site in its promoter

   Depositor

   Brian Pfleger

   Article

   Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

   Insert

   mCherry
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBz84

   Plasmid

   #226318

   Purpose

   E. coli protein expression for protein purification

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Insert

   6xHis- (wtMR1-6)- strep
   

   Use

   Tags

   6xHis and Strep

   Expression

   Bacterial

   Mutation

   N-terminal domain deleted, C-terminal domain dele…

   Promoter

   T7

   Available since

   Oct. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET21a-KstR

   Plasmid

   #51179

   Depositor

   Christopher Voigt

   Article

   Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

   Insert

   KstR
   

   Use

   Synthetic Biology

   Tags

   6x-his

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET21a-CampR

    Plasmid

    #51162

    Depositor

    Christopher Voigt

    Article

    Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

    Insert

    CampR
    

    Use

    Synthetic Biology

    Tags

    6x-his

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCellFree_G03 PC

    Plasmid

    #67132

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    PC (PC Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pJB75

    Plasmid

    #226320

    Purpose

    E. coli protein expression for protein purification

    Depositor

    David Savage

    Article

    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

    Insert

    6xHis-(Y to A MR1-6)-strep
    

    Use

    Tags

    6xHis and Strep

    Expression

    Bacterial

    Mutation

    N-terminal domain deleted, C-terminal domain dele…

    Promoter

    T7

    Available since

    Oct. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFH77

    Plasmid

    #125816

    Purpose

    Level0 enCas9-PolI3M-TBD, wheat codon optimized

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    enCas9-PolI3M-TBD, wheat codon optimized
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Ki67-mNeonGreen-IRESpuro2

    Plasmid

    #183737

    Purpose

    Expresses Ki67-mNeonGreen

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    MKI67 (MKI67 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBP-T7RNAP

    Plasmid

    #74096

    Purpose

    T7 RNA polymerase PCR cloned from BL21 (DE3) genomic DNA

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    T7 RNAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    None

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET21a - TR

    Plasmid

    #89621

    Purpose

    [Edit] Expresses recombinant 6HIS tagged T. cruzi trypanothione reductase protein in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Thioredoxin
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. H2B-mNeonGreen-IRESpuro2

    Plasmid

    #183745

    Purpose

    Expresses H2B-mNeonGreen

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    H2B (H2BC16P Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. hMTP-flag

    Plasmid

    #138335

    Purpose

    Expresses MTP

    Depositor

    Mahmood Hussain

    Article

    Rava et al J Biol Chem. 2006 Apr 21;281(16):11019-2

    Insert

    MTTP (MTTP Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pgRNA

    Plasmid

    #215560

    Purpose

    Constitutively expressed sgRNA targeting a desired gene (this case pta), ColE1 origin, KanR

    Depositor

    Brian Pfleger

    Article

    Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

    Insert

    gRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET21a - NR

    Plasmid

    #89625

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi nitrate reductase in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Nitrate reductase
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge