We narrowed to 28,196 results for: NOL

1. PX551

   Plasmid

   #60957

   Purpose

   pAAV-pMecp2-SpCas9-spA (AAV-SpCas9). AAV plasmid expressing Cas9 in neurons under control of truncated mecp2 promoter.

   Depositor

   Feng Zhang

   Article

   Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103

   Insert

   SpCas9
   

   Use

   AAV and CRISPR

   Tags

   HA

   Expression

   Mammalian

   Promoter

   pMecp2

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGWB441

   Plasmid

   #74827

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with eYFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   Oct. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCaRNA1&2

   Plasmid

   #86087

   Purpose

   Simultaneous expression of cDNAs of complete genomic RNA1 and RNA 2 of PNRSV (Pch12 isolate) in transfected cells

   Depositor

   Aiming Wang

   Article

   Cui et al Plant Biotechnol J. 2016 Aug 27. doi: 10

   Insert

   RNA1, RNA2
   

   Expression

   Plant

   Promoter

   35S

   Available Since

   Feb. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCaRNA3

   Plasmid

   #86088

   Purpose

   Expression of cDNA of full-length genomic RNA3 of PNRSV (Pch12 isolate) in transfected cells

   Depositor

   Aiming Wang

   Article

   Cui et al Plant Biotechnol J. 2016 Aug 27. doi: 10

   Insert

   RNA3
   

   Expression

   Plant

   Promoter

   35S

   Available Since

   Jan. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCast

   Plasmid

   #200169

   Purpose

   E. coli/Yeast shuttle vector carrying CloNAT marker and Spcas9 allowing cloning of gRNA flanked by tRNA promoter and HDV ribozyme

   Depositor

   Yong-Su Jin

   Article

   Kuanyshev et al Biotechnol Bioeng. 2021 Jan;118(1):372-3

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   June 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. hSTARR-seq_ORI vector

   Plasmid

   #99296

   Purpose

   Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

   Depositor

   Alexander Stark

   Article

   Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

   Type

   Empty backbone

   Use

   Hstarr-seq screening vector

   Expression

   Mammalian

   Available Since

   Dec. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pR26 CAG/GFP Dest

   Plasmid

   #74281

   Purpose

   Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, GFP, for gateway cloning

   Depositor

   Ralf Kuehn

   Article

   Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

   Insert

   Rosa26 5-homology region
   

   Use

   Mouse Targeting

   Available Since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+)/AUG-nLuc-3XFLAG

   Plasmid

   #127299

   Purpose

   Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJRH-1362 scFv entry plasmid

   Plasmid

   #201912

   Purpose

   Entry vector for cloning single chain variable fragments displayed on the human CD8a hinge and transmembrane domain (CAG promoter)

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Insert

   Stuffer sequence (drop out for scFv cloning) + CD8 hinge and transmembrane domain
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pComb3XTT

    Plasmid

    #63891

    Purpose

    Backbone of pComb3X containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control and PCR template for human antibody constant regions (kappa and CH1)

    Depositor

    Carlos Barbas

    Article

    Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

    Insert

    TT Fab
    

    Use

    Phage display

    Tags

    6xHis, HA tag, and geneIII

    Expression

    Bacterial

    Promoter

    lacZ

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. ABE8e(TadA-8e V106W)

    Plasmid

    #138495

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e V106W)-nSpCas9
    

    Expression

    Mammalian

    Mutation

    TadA-8e V106W

    Available Since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDriver

    Plasmid

    #165149

    Purpose

    Driver plasmid for PROBER encoding SV40 Large T-antigen

    Depositor

    Paul Khavari

    Article

    Mondal et al Nat Methods. 2022 Aug;19(8):959-968. doi

    Insert

    SV40 Large T-antigen
    

    Expression

    Mammalian

    Available Since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pXPR_120

    Plasmid

    #96917

    Purpose

    for CRISPRa, lentiviral expression of dCas9-VPR 2A BlastR

    Depositor

    John Doench, David Root

    Article

    Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    VPR

    Expression

    Mammalian

    Mutation

    D10A, N863A

    Promoter

    EF1a

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAnc80L65AAP

    Plasmid

    #92307

    Purpose

    Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct with endogenous AAP

    Depositor

    Luk Vandenberghe

    Article

    Landegger et al Nat Biotechnol. 2017 Mar;35(3):280-284.

    Insert

    Ancestral AAV Capsid Anc80L65AAP
    

    Use

    AAV and Synthetic Biology

    Expression

    Mammalian

    Promoter

    Rep2

    Available Since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAP01

    Plasmid

    #59981

    Purpose

    The function of this material is to facilitate the capture and expression of bacterial gene clusters.

    Depositor

    Bradley Moore

    Article

    Yamanaka et al Proc Natl Acad Sci U S A. 2014 Feb 4;111

    Type

    Empty backbone

    Use

    Synthetic Biology; Transformation-associated reco…

    Tags

    no

    Expression

    Bacterial and Yeast

    Promoter

    no

    Available Since

    Nov. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRDA_052

    Plasmid

    #136474

    Purpose

    Lentiviral expression of AsCas12a gRNA

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

    Insert

    Puromycin resistance
    

    Use

    Lentiviral and Synthetic Biology

    Promoter

    EF1a

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-SpRY-ABE8e

    Plasmid

    #185671

    Purpose

    Expresses SpRY-ABE8e in mammalian cells

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    ecTadA(8e)-nSpRY
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    SpRY D10A

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMK381 (AAVS1 CMV-OsTIR1F74G)

    Plasmid

    #140536

    Purpose

    AAVS1 CMV-OsTIR1(F74G)

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    CMV-OsTIR1(F74G)
    

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAF0451 Si74_attP-mCherry donor

    Plasmid

    #193470

    Purpose

    Promoterless mcherry donor for Si74

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Si74_attP
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAF0474 Ef1a-Si74_attB acceptor

    Plasmid

    #193475

    Purpose

    Acceptor plasmid for 3 plasmid recombination assay

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Si74_attB
    

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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