161,398 results

1. pLG002 (BREX type I)

   Plasmid

   #157880

   Purpose

   Expresses the BREX type I defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   BREX type I
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. MAC_C_TYRO3

   Plasmid

   #187793

   Purpose

   MAC-tagged gene expression of human TYRO3

   Depositor

   Markku Varjosalo

   Article

   Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

   Insert

   TYRO3 (TYRO3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJ204-loxP-EF1a-tagBFP-HygR-lox2272-BGHpA-siteT9-HDR

   Plasmid

   #154827

   Purpose

   Landing pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site T9)

   Depositor

   Lise Marie Grav

   Article

   Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

   Inserts

   TagBFP
   HygR
   ZsGreen1-DR
   

   Use

   CRISPR and Cre/Lox

   Tags

   PEST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, EF1a, and SV40 early promoter

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3 HA 14-3-3 sigma

   Plasmid

   #11946

   Depositor

   Michael Yaffe

   Article

   Wilker et al J Biol Chem. 2005 May 13. 280(19):18891-

   Insert

   Stratifin (SFN Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSK+ chickBMPR-1B CA

   Plasmid

   #49528

   Purpose

   cloning vector for constitutively active chick BMP receptor 1B

   Depositor

   Peter ten Dijke, Lee Niswander

   Article

   Varley et al Dev Biol. 1998 Apr 1;196(1):107-18.

   Insert

   BMPR-1B CA (BMPR1B Chicken)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   constitutively active mutation (Q203D); also P2A,…

   Promoter

   T3

   Available since

   May 20, 2014

   Availability

   Academic Institutions and Nonprofits only

6. mCherry-Desmoglein2-C-18

   Plasmid

   #55031

   Purpose

   Localization: Desmosomes, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Desmoglein2 (DSG2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-smURFP-StagRFP-EKAR-EV

   Plasmid

   #173019

   Purpose

   Red/far-red FRET-based ERK activity reporter for multiplexed imaging applications.

   Depositor

   Jin Zhang

   Article

   Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

   Insert

   smURFP-StagRFP-EKAR-EV
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDisplay-mgSrtA

   Plasmid

   #125792

   Purpose

   Transfected into cell of interest for membrane expression. The encoded mgSrtA could enable labeling of the interacting cells which is useful for recording cell-cell interactions.

   Depositor

   P. Chen

   Article

   Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18

   Insert

   mgSrtA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. T/brachyury(2A-Dre)

   Plasmid

   #140761

   Purpose

   Targeting vector to T/brachyury gene locus for induction of P2A-fused Dre, with PGK-EM7-puroR drug resistant cassette flanked by FRT(F3) sequence.

   Depositor

   Hideyuki Okano

   Article

   Serizawa et al Development. 2019 Nov 4;146(21). pii: de

   Insert

   Dre
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Endogenous T/brachyury

   Available since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAdTrack

    Plasmid

    #16404

    Purpose

    Shuttle vector for us in AdEasy System. Used for expression of transgenes under a chosen (non-CMV) promoter when a GFP tracer is desired.

    Depositor

    Bert Vogelstein

    Article

    He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

    Type

    Empty backbone

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEJS477-pHAGE-TO-Spy dCas9 3XmCherry-SgRNA/Telomere-All-in-one

    Plasmid

    #85717

    Purpose

    Expresses dSpyCas9-3XmCherry and telomere-targeting Spy sgRNA

    Depositor

    Erik Sontheimer

    Article

    Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

    Inserts

    Sp dCas9
    telomere sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    3XmCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO and U6

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-Mff

    Plasmid

    #49153

    Purpose

    mammalian expression of Mff fused to GFP

    Depositor

    Gia Voeltz

    Article

    Friedman et al Science. 2011 Oct 21;334(6054):358-62. d

    Insert

    Mff (MFF Human)
    

    Use

    Tags

    acGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLminP_Luc2P_RE53

    Plasmid

    #90398

    Purpose

    STAT1 - STAT1 gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    STAT1

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-ExRai-CKAR

    Plasmid

    #118409

    Purpose

    cpGFP-based excitation-ratiometric biosensor for monitoring Protein Kinase C activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    ExRai-CKAR
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pC016 - LwCas13a guide expression backbone with U6 promoter

    Plasmid

    #91906

    Purpose

    Backbone for cloning LwCas13a guides under U6 promoter

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Has Service

    Cloning Grade DNA

    Insert

    LwCas13a Guide site
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. phGluc

    Plasmid

    #22522

    Depositor

    Christopher Buck

    Article

    Pastrana et al PLoS Pathog. 2009 Sep . 5(9):e1000578.

    Insert

    Gaussia luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYPQ131B

    Plasmid

    #69281

    Purpose

    Golden Gate entry vector; 1st gRNA under AtU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG_smFP Myc

    Plasmid

    #59757

    Purpose

    encodes multiple copies of an epitope tag Myc

    Depositor

    Loren Looger

    Article

    Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

    Insert

    smFP _Myc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Plenti-ARE-HSVTK-luc

    Plasmid

    #161789

    Purpose

    Expresses HSV-TK and Luciferase in an NRF2 dependent manner. Also, expresses cherry and blasticidin resistance selection markers

    Depositor

    Hans-Guido Wendel

    Article

    Sanghvi et al Cell. 2019 Aug 8;178(4):807-819.e21. doi

    Inserts

    Luciferase-T2A-HSV-TK
    mCherry P2A blasticidin
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and Murine Gclc ARE

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV2-ProC3-Cre/mCherry

    Plasmid

    #126006

    Purpose

    Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

    Depositor

    Botond Roska

    Article

    Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

    Insert

    Cre-2A-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    ProC3

    Available since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. HSC1-GiP

    Plasmid

    #58254

    Purpose

    This self-inactivated retroviral vector has an internal human EF1alpha promoter driving the expression of a bicistronic EGFP ires Puromycin cassette

    Depositor

    James Ellis

    Article

    Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

    Insert

    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human EF1-alpha

    Available since

    Oct. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA5/FRT/TO_HaloTag7_LamB1

    Plasmid

    #175528

    Purpose

    Expression of HaloTag7 at the nuclear lamina of the mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    HaloTag7-LamB1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3puro-mGFP

    Plasmid

    #225446

    Purpose

    Express mEGFP-fusion protein of interest

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Type

    Empty backbone

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCalN-ddGFP-A

    Plasmid

    #40290

    Depositor

    Robert Campbell

    Article

    Alford et al ACS Synth Biol. 2012 Dec 21;1(12):569-75

    Insert

    Calnexin (CANX Human)
    

    Use

    Tags

    ddGFP-A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLJM1 Flag Raptor

    Plasmid

    #26633

    Depositor

    David Sabatini

    Article

    Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

    Insert

    raptor (RPTOR Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDONR223_NRAS_p.Q61R

    Plasmid

    #81652

    Purpose

    Gateway Donor vector containing NRAS, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    NRAS (NRAS Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Q61R

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGEMT-PTE2A

    Plasmid

    #111773

    Purpose

    Quadcistronic construct: Co-express four genes by three 2A peptides -- P2A, T2A and E2A

    Depositor

    Li Qian

    Article

    Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

    Type

    Empty backbone

    Use

    Cloning intermediate

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMpGWB236

    Plasmid

    #68627

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    TagRFP

    Expression

    Plant

    Mutation

    Promoter

    MpEF1alpha promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pKH144_mCherry-YIPF4 WT

    Plasmid

    #214972

    Purpose

    mCherry tagged YIPF4

    Depositor

    Wade Harper

    Article

    Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

    Insert

    mCherry-YIPF4 (YIPF4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV5B-Flag-Smurf2 wt

    Plasmid

    #11746

    Depositor

    Jeff Wrana

    Article

    Kavsak et al Mol Cell. 2000 Dec . 6(6):1365-75.

    Insert

    SMURF2 (SMURF2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 12, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge