We narrowed to 17,080 results for: Por

1. pNHT7::RPL15::sNluc2_STOP

   Plasmid

   #186767

   Purpose

   An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc2) containing a premature stop codon.

   Depositor

   Bo Wang

   Article

   Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

   Insert

   Nanoluciferase
   

   Use

   Tags

   Expression

   Mutation

   Premature stop codon at residue 16

   Promoter

   T7

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNHT7::RPL15::sGluc

   Plasmid

   #186769

   Purpose

   An mRNA production vector encoding planarian codon-optimized gaussia luciferase (sGluc) with RPL15 5' and 3' UTRs.

   Depositor

   Bo Wang

   Article

   Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

   Insert

   Gaussia luciferase
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET24a-LaM4-2xTS

   Plasmid

   #162779

   Purpose

   Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to two tyrosine sulfation (TS) motifs. LaM4-2xTS also contains a T7, HA, BAP and His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

   Insert

   anti-mCherry nanobody fused to two TS sites, T7, HA, BAP and His6 epitope
   

   Use

   Tags

   BAP, HA, His6, T7, and TS site from proCCK

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFlare9A-CD45i4e5i5

   Plasmid

   #131834

   Purpose

   A reporter construct containing the Exon 5 of PTPRC (CD45), which contains a CTCF binding site.

   Depositor

   Mark Minden

   Article

   Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

   Insert

   PTPRC (PTPRC Human)
   

   Use

   Reporter construct

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFBP-2_6m.mutant

   Plasmid

   #162842

   Purpose

   Yeast expression of the mutant #2_6m for the RNA-based sensor of fructose-1,6-bisphosphate #2_6

   Depositor

   Matthias Heinemann

   Article

   Ortega et al Cell Chem Biol. 2021 Apr 22. pii: S2451-

   Insert

   2_6m_RNA-device
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-Hygro-EF1A-KR-Linker-eGFP

   Plasmid

   #176066

   Purpose

   EGFP fused to the C-terminus of a KR domain & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   KR
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available Since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLentiCRISPRv1_COX4

   Plasmid

   #177983

   Purpose

   lentiviral vector expressing Cas9 and a sgRNA targeting COX4

   Depositor

   Jessica Spinelli, David Sabatini

   Article

   Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

   Insert

   sgRNA targeting COX4
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   U6

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-OSF-PP-squirrel-monkey-CHMP3(155)

   Plasmid

   #154183

   Purpose

   expresses squirrel monkey CHMP3(155) in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

   Insert

   CHMP3(155)
   

   Use

   Tags

   FLAG, Strep-Tag II, and preScission site

   Expression

   Mammalian

   Mutation

   C-terminal truncation of CHMP3 at amino acid 155

   Promoter

   Available Since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. EGFP-uTEV1-SQSfl

   Plasmid

   #171010

   Purpose

   HiLITR protease with full-length SQS targeting information (ER)

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   EGFP-uTEV1-SQS(FL)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pTRE-tight (TetOn)

   Available Since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGMC00008

    Plasmid

    #166724

    Purpose

    sgRNA cloning backbone

    Depositor

    Raj Chari

    Article

    Choi et al Nat Commun. 2020 Jun 1;11(1):2718. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pWZL N-Myc-hPOT1_K90E-IRES_hygro

    Plasmid

    #166407

    Purpose

    Express hPOT1 K90E in mammalian cells

    Depositor

    Agnel Sfeir

    Article

    Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

    Insert

    hPOT1 (POT1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    K90E

    Promoter

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. MPC027: pAAV-CAG-FLEX-sNovArch-PP-Citrine_KV2.1TS-ER2 (soma targeting)

    Plasmid

    #153196

    Purpose

    encodes pAAV soma targeted NovArch

    Depositor

    Adam Cohen

    Article

    Chien et al Sci Adv. 2021 May 5;7(19). pii: 7/19/eab

    Insert

    sNovArch
    

    Use

    AAV

    Tags

    citrine

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB-TAC-OKMS-KRAB-dCas9

    Plasmid

    #120356

    Purpose

    piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). KRAB-dCas9 is constitutively expressed.

    Depositor

    Knut Woltjen

    Article

    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

    Insert

    OKMS cassette
    

    Use

    CRISPR; Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Klf4 [10-483]

    Promoter

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB-TAC-OK+9MS-KRAB-dCas9

    Plasmid

    #120357

    Purpose

    piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). KRAB-dCas9 is constitutively expressed.

    Depositor

    Knut Woltjen

    Article

    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

    Insert

    OK+9MS cassette
    

    Use

    CRISPR; Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Klf4 [1-483]

    Promoter

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. THI ONLY pALD4 T-

    Plasmid

    #161497

    Purpose

    Non activation and non targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T-_gRNA_MS2

    Depositor

    Brigitte Gasser, Diethard Mattanovich

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2 (non activation control NAC)
    gRNA (Non targeting control NTC)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pALD4, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. GSX2-P2A-tGFP-DTA

    Plasmid

    #161750

    Purpose

    tGFP plasmid for the c-terminal targeting of human GSX2 locus

    Depositor

    Elena Cattaneo

    Article

    Besusso et al Stem Cell Res. 2020 Dec;49:102016. doi:

    Insert

    turboGFP
    

    Use

    CRISPR

    Tags

    P2A-tGFP

    Expression

    Mammalian

    Mutation

    Promoter

    endogenous GSX2 promoter

    Available Since

    Dec. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. DYNLL2

    Plasmid

    #132531

    Purpose

    Human LC8-2, codon optimised for Sf9 expression.

    Depositor

    Andrew Carter

    Article

    Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

    Insert

    DYNLL2 (DYNLL2 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Codon optimised for Sf9 expression

    Promoter

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. DYNLT3

    Plasmid

    #132532

    Purpose

    Human TCTEX-3, codon optimised for Sf9 expression.

    Depositor

    Andrew Carter

    Article

    Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

    Insert

    DYNLT3 (DYNLT3 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Codon optimised for Sf9 expression

    Promoter

    Available Since

    Oct. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. DYNLRB2

    Plasmid

    #132534

    Purpose

    Human Rbl-2, codon optimised for Sf9 expression.

    Depositor

    Andrew Carter

    Article

    Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

    Insert

    DYNLRB2 (DYNLRB2 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Codon optimised for Sf9 expression

    Promoter

    Available Since

    Oct. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGL3 T2P 800

    Plasmid

    #131147

    Purpose

    To investigate activity of a 800 bp region upstream of mouse Trib2.

    Depositor

    Karen Keeshan

    Article

    Rishi et al Blood. 2014 Apr 10;123(15):2389-400. doi

    Insert

    -800 - 0 of mTrib2 upstream of gene transcriptional start site (Trib2 Mouse)
    

    Use

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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