We narrowed to 25,117 results for: p

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pAC025

Plasmid

#227597

Purpose

Lentiviral backbone used when cloning pooled prime editing guide RNA libraries already containing a tevopreQ1 RNA motif

Depositor

Brittany Adamson

Article

Cirincione et al Nat Methods. 2024 Nov 19. doi: 10.1038/s

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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DT044A

Plasmid

#159405

Purpose

Inducible Perfringolysin O (PFO) expression and purification, controlled under hybrid IPTG-regulated T7/LacO promoter (pRT30)

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.T7-LacO-6xHis-PFO (Pfo, Clostridium perfringens)

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

T7/LacO promoter

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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PB-CT3G-ERP2-MG

Plasmid

#175501

Purpose

All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3G-mCMV

Available Since

Oct. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pEYFP-N1-eKR2

Plasmid

#115337

Purpose

Expresses enhanced sodium pump for light-driven extrusion of sodium; activation with green light; codon-optimized for human/mouse

Depositor

Peter Hegemann

Article

Grimm et al Sci Rep. 2018 Jun 18;8(1):9316. doi: 10.

Insert

eKR2

Tags

EYFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS3

Plasmid

#127128

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS3 (RPS3 Human)

Use

Lentiviral

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)_No_FLAG

Plasmid

#79877

Purpose

FLAGless construct expressing high specificity eSpCas9(1.1). Px330-like plasmid.

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Type

Empty backbone

Use

CRISPR

Tags

Untagged eSpCas9(1.1)

Expression

Mammalian

Promoter

CBh

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mNeonGreen-LEHD-NanoLuc

Plasmid

#98289

Purpose

caspase-9 BRET Sensor

Depositor

Maarten Merkx

Article

den Hamer et al ACS Sens. 2017 Jun 23;2(6):729-734. doi:

Insert

mNeonGreen-LEHD-NanoLuc

Tags

His-tag

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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NLS-tdPCP-GFP

Plasmid

#183934

Purpose

PCP tandem dimer binding to PP7 RNA stem loops; labeled with GFP

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

tdPCP-GFP

Tags

NLS

Expression

Mammalian

Mutation

none

Promoter

CMV (TATA box removed)

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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tetO-Myocd

Plasmid

#46033

Depositor

John Gearhart

Article

Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

Insert

Myocd (Myocd Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

tetO

Available Since

July 9, 2013

Availability

Academic Institutions and Nonprofits only

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pEGB 35S:DsRed:Tnos (GB0361)

Plasmid

#68220

Purpose

Transcriptional unit for DsRed plant expression driven by the 35S promoter

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

DsRed

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

35S

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pPPC019.AAV

Plasmid

#171147

Purpose

Expression of J1-BBa_J23117-mRFP and hAAVS1 scRNA on pRK2-KmR plasmid

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Inserts

hAAVS1 scRNA
J1-BBa_J23117-mRFP

Use

CRISPR

Expression

Bacterial

Promoter

BBa_J23119 and J1-BBa_J23117

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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CVS-N2c-nl.EGFP-SypGFP

Plasmid

#172380

Purpose

Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

nuclear-localized EGFP + SypEGFP

Use

Neurotropic virus

Available Since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pVH64-Tier1-PSV40-Citrine_p2a_SEAP

Plasmid

#169558

Purpose

Tier-1 vector encoding PSV40-driven Citrine-p2A-SEAP expression (PSV40-Citrine-p2A-SEAP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PSV40-driven citrine-P2A-SEAP fusion construct

Expression

Mammalian

Promoter

PSV40

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pET28_Caspase-4_K356D

Plasmid

#214312

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (CASP4 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

K356D

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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Twist-siRNA3

Plasmid

#1784

Depositor

Bob Weinberg

Article

Yang et al Cell 2004 Jun 25;117(7):927-39.

Insert

siTwist (Twist1 Mouse)

Expression

Mammalian

Available Since

April 28, 2005

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-rSyntaxin-3a

Plasmid

#195206

Purpose

Mammalian expression vector for GFP tagged Syntaxin-3a.

Depositor

David Bryant, Thomas Weimbs

Article

Giovannone et al J Biol Chem. 2018 Apr 13;293(15):5478-54

Insert

STX3A (Stx3 Rat)

Expression

Mammalian

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-2mCh-PS

Plasmid

#112268

Purpose

IDOCKS Acceptor Construct

Depositor

Ryohei Yasuda

Article

Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

Insert

two copies of mCherry followed by a pseudosubstrate peptide for PKC

Tags

mCh

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pMSSM16: pCMV-HA-NLS-SpuFz1

Plasmid

#205271

Purpose

Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Tags

HA, NLS(Makkerh)

Expression

Mammalian

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pMetTU1-A_Sv5K_araC-PBAD_MCD1_AnaA-T6A-I48V_AnaCNJKFGW_Bba-B0015

Plasmid

#202023

Purpose

Expresses Anabaena flos-aquae ARG cluster (GvpA T6A-I48V mutant) in E. coli

Depositor

Mikhail Shapiro

Article

Hurt et al bioRxiv 2024.03.30.587094

Insert

Anabaena flos-aquae ARG cluster (GvpA T6A-I48V mutant)

Expression

Bacterial

Mutation

GvpA T6A-I48V

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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TagRFP-T-VAPB(1- 218)- eMagB

Plasmid

#162255

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

TagRFP-T-VAPB(1- 218)- eMagB

Expression

Mammalian

Promoter

beta-actin promoter

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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