165,545 results

1. pProEx RalA(G23C) CL

   Plasmid

   #224263

   Purpose

   Protein purification of RalA (G23C)

   Depositor

   Kevan Shokat

   Article

   Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

   Insert

   RalA (RALA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   G23C

   Promoter

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBAD-bARGSer-AxeTxe

   Plasmid

   #192473

   Purpose

   Second-generation bacterial acoustic reporter gene derived from Serratia

   Depositor

   Mikhail Shapiro

   Article

   Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Inserts

   bARGSer
   AxeTxe
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   the gene Ser39006_001280 was deleted from the clu…

   Promoter

   Native promoter from Enterococcus faecium and pBAD

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGP-AAV-syn-jGCaMP7f-WPRE

   Plasmid

   #104488

   Purpose

   AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, AAV8, and AAV9

   Insert

   jGCaMP7f
   

   Use

   AAV

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. UKNP1.16.3

   Plasmid

   #98188

   Purpose

   Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

   Depositor

   Jonathan Ball

   Article

   Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

   Insert

   HCV E1E2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. TDP-43 mRuby

   Plasmid

   #216225

   Purpose

   TDP-43 with a C-terminal mRuby tag for expression in mammalian cells.

   Depositor

   Jennifer Lee

   Article

   Shadish et al Biophys Chem. 2024 Jan 23;307:107191. do

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   mRuby

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. CL20_mEGFP_CUX1_NUTM1

   Plasmid

   #205800

   Purpose

   Express mEGFP-tagged fusion protein, CUX1_NUTM1 from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   CUX1_NUTM1 (CUX1, NUTM1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLX304_CdTag-HA_EmptyVector

   Plasmid

   #221466

   Purpose

   Gateway backbone for C-terminal dTag cloning

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   FKBP F36V

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE-TO-dCas9

   Plasmid

   #75381

   Purpose

   dCas9

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

   Insert

   Sp dCas9
   

   Use

   Lentiviral

   Tags

   no

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TO

   Available since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. LiRT

   Plasmid

   #214075

   Purpose

   Luminescent reporter system to measure EJC-independent NMD activity. Intron present in 5'UTR

   Depositor

   Sujatha Jagannathan

   Article

   Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

   Insert

   Firefly luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Linker sequence present between firefly luciferas…

   Promoter

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. LiRT_RTctrl

    Plasmid

    #214078

    Purpose

    Luminescent reporter system, readthrough control, NMD negative control. Intron present in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    Firefly luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Linker sequence present between firefly luciferas…

    Promoter

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. LiRTi

    Plasmid

    #214076

    Purpose

    Luminescent reporter system to measure EJC-enhanced NMD activity. Intron present in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    Firefly luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Linker sequence present between firefly luciferas…

    Promoter

    Available since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. LiRTX_ctrl

    Plasmid

    #214077

    Purpose

    Luminescent reporter system, NMD negative control. Intron present in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    Firefly luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Remnant of linker sequence present at the end of …

    Promoter

    Available since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCR1326

    Plasmid

    #111131

    Purpose

    Expresses UBE2T.

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    UBE2T (UBE2T Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSEED-mScarlet3

    Plasmid

    #219640

    Purpose

    Permits GoldenGate assembly of donor plasmids to knock-in mScarlet3 (BsmBI) using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    mScarlet SEED Cassette
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDH-puro-CMV-VC3AI

    Plasmid

    #78907

    Purpose

    Indicator of caspase-3-like protease activity

    Depositor

    Binghui Li

    Article

    Zhang et al Nat Commun. 2013;4:2157. doi: 10.1038/nc

    Insert

    VC3AI
    

    Use

    Lentiviral

    Tags

    HA and Myc

    Expression

    Mutation

    S320A, deletion of AA1-200

    Promoter

    CMV

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. TFORF1106

    Plasmid

    #143762

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    EPAS1 (EPAS1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO-Tet-On-ARNTL-shRNA1

    Plasmid

    #166913

    Purpose

    Lentivirus for inducible knockdown of human ARNTL(BMAL1)

    Depositor

    Roland Friedel

    Insert

    ARNTL shRNA (BMAL1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-hATG7wt

    Plasmid

    #87867

    Purpose

    Express a wild type human Atg7/Apg7 in mammalian cells

    Depositor

    Isei Tanida, Eiki Kominami

    Article

    Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

    Insert

    ATG7 (ATG7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. SPYtag-Histag-Avidin

    Plasmid

    #214836

    Purpose

    This plasmid encodes a protein (SPYtag-Histag-Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

    Depositor

    Hironori Funabiki

    Article

    Arimura et al bioRxiv 2024.01.21.576499.

    Insert

    SPYtag-Histag-Avidin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-Ef1a-DIO ChETA-EYFP (AAV5)

    Viral Prep

    #26968-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-Ef1a-DIO ChETA-EYFP (#26968). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO ChETA-EYFP plasmid DNA. EF1a-driven, Cre-dependent, ChETA fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Article

    Gunaydin et al Nat Neurosci. 2010 Mar;13(3):387-92. doi

    Promoter

    EF1a

    Tags

    EYFP (Cre-dependent)

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

21. CIDAR MoClo Extension, Volume I

    Plasmid Kit

    #1000000161

    Purpose

    This kit contains 110 plasmids designed to quickly and efficiently build a wide variety of gene expression constructs using the CIDAR-standard MoClo system.

    Depositor

    Richard Murray

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

22. CIBN-CAAX

    Plasmid

    #79574

    Purpose

    expression of N-terminal portion of CIB1 with a C-terminal CAAX box from KRras for plasma-membrane targeting

    Depositor

    Pietro De Camilli, Olof Idevall-Hagren

    Article

    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

    Insert

    CIBN (CIB1 Mustard Weed)
    

    Use

    Tags

    C-terminal CAAX-box

    Expression

    Mammalian

    Mutation

    K93A, R94A, K106A, K107A (please see depositor co…

    Promoter

    CMV

    Available since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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23. PGK EGFP

    Plasmid

    #216110

    Purpose

    PGK EGFP

    Depositor

    Erika Holzbaur

    Article

    Dou et al J Cell Biol. 2024 Jun 3;223(6):e20230709

    Insert

    PGK promoter
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. pRK5-myc-PAT1

    Plasmid

    #88856

    Purpose

    myc-TAG overexpression

    Depositor

    David Sabatini

    Article

    Zoncu et al Science. 2011 Nov 4;334(6056):678-83.

    Insert

    SLC36A1 (SLC36A1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHR_XPR2_hrGFP

    Plasmid

    #84614

    Purpose

    HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into XPR2 locus

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    hrGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    UAS1B8-TEF(136)

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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26. pQE-60NA-msGFP2

    Plasmid

    #135301

    Purpose

    Encodes a photostable monomeric superfolder GFP

    Depositor

    Benjamin Glick

    Article

    Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

    Insert

    msGFP2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLJM1-FLAG-GFP-OSBP

    Plasmid

    #134659

    Purpose

    Flag and GFP-tagged OSBP

    Depositor

    Roberto Zoncu

    Article

    Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

    Insert

    OSBP (OSBP Human)
    

    Use

    Lentiviral

    Tags

    FLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV- κLight1.3

    Plasmid

    #201223

    Purpose

    Express kLight1.3 biosensor in mammalian cells

    Depositor

    Lin Tian

    Article

    Dong et al Nat Neurosci. 2024 Jul 15. doi: 10.1038/

    Insert

    κLight1.3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRN12

    Plasmid

    #84456

    Purpose

    shuttle plasmid for sarA P1-mAmetrine expression

    Depositor

    Reindert Nijland

    Article

    de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

    Insert

    mAmetrine
    

    Use

    Shuttle vector e.coli-s.aureus, expression of sgf…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLeAPS-GFP-blast

    Plasmid

    #182230

    Purpose

    Lentiviral transfer plasmid for the LeAPS packaging system; encodes GFP-P2A-blasticidin transgene

    Depositor

    Michael Birnbaum

    Article

    Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

    Insert

    GFP-P2A-Blasticidin
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge