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AAVS1-iPE2-Hygro

Plasmid

#214019

Purpose

inducible PE2 system for controllable prime editing; this plasmid is used to insert PE2 prime editor in one allele of AAVS1 locus

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

PE2

Expression

Mammalian

Promoter

TRE-tight

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA-S1b (BsaI)-CBh-eCas12f1a

Plasmid

#233079

Purpose

Expression vector for sgRNA-S1b and eCas12f1a

Depositor

Kyoungmi Kim

Article

Park et al Nat Commun. 2025 Jan 15;16(1):677. doi:

Insert

U6-sgRNA-S1b (BsaI)-Cbh-bpNLS-eCas12f1a-2xFLAG-P2A-EGFP

Use

CRISPR

Tags

2xFLAG

Expression

Mammalian

Promoter

hU6, CBh

Available Since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-48xCCAT1 array

Plasmid

#236369

Purpose

Expression of array of 48 crRNAs targeting CCAT1

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

48xCCAT1 crRNA array (CCAT1 Human)

Use

CRISPR and Lentiviral

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

Plasmid

#42337

Purpose

Dual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Available Since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

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1313_pAAV-U6-SA-BbsI-MluI-gRNA-HLP-SACas9-HA-OLLAS-spA

Plasmid

#109314

Purpose

Plasmid for liver-specific expression of AAV SaCas9 with a BbsI cloning site for a gRNA

Depositor

William Lagor

Article

Li et al Mol Ther Methods Clin Dev. 2018 Dec 6;12

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pTW2065

Plasmid

#236574

Purpose

ISYmu1 PDS3 gRNA2 TRV2 Architecture_A

Depositor

Steven Jacobsen

Article

Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

Insert

ISYmu1 TnpB

Expression

Plant

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-hU6-enhanced_gRNA-BFP-P2A-PuroR

Plasmid

#230935

Purpose

gRNA cloning vector encoding BFP and puromycin resistance

Depositor

Charles Gersbach

Article

Liu et al Cell Genom. 2025 Feb 4:100766. doi: 10.1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

TagBFP

Promoter

hU6

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCV(gfp)U6sgRNA5(BbsI)-PGKpuroBFP

Plasmid

#102798

Purpose

Retrovirus for delivery of one sgRNA (self-targeting) - GFP-targeting control plasmid contains GFP instead of PuroR

Depositor

Sarah Teichmann

Article

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Inserts

sgRNA
EGFP

Use

CRISPR and Retroviral

Expression

Mammalian

Mutation

H231L

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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T-STOP sgRNA-1

Plasmid

#83346

Purpose

The sgRNA at the stop codon of human Brachyury(T) locus

Depositor

James Thomson

Article

Chu et al Genome Biol. 2016 Aug 17;17(1):173. doi:

Insert

T-STOP sgRNA

Expression

Mammalian

Promoter

U6

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pICSL11059

Plasmid

#68263

Purpose

Level 1 Golden Gate Cassette: Plant hygromycin (with intron) resistance cassette

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter:35s + 5'UTR:Omega + CDS:hptIIwintron + 3'UTR/terminator:35s

Use

Synthetic Biology

Expression

Plant

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pDIRECT_25H

Plasmid

#91145

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers

Use

CRISPR

Expression

Plant

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pQTEV-HSPB8

Plasmid

#34710

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

heat shock 22 kDa protein 8 (HSPB8 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pL-CRISPR.SFFV.tRFP

Plasmid

#57826

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. SFFV Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
SFFV
P2A-tRFP

Use

CRISPR and Lentiviral

Tags

FLAG

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pCSF107mT-GATEWAY-3'-Myc tag

Plasmid

#67617

Purpose

A pCS2 based vector to generate a 3'-Myc tag using GATEWAY cloning technology

Depositor

Todd Stukenberg

Article

Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

Type

Empty backbone

Use

In vitro transcription or translation off of an s…

Tags

C-terminal Myc

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pTW2157

Plasmid

#236581

Purpose

ISYmu1 CHLl1 gRNA7 TRV2 Architecture_B

Depositor

Steven Jacobsen

Article

Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

Insert

ISYmu1 TnpB

Expression

Plant

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pTW2156

Plasmid

#236580

Purpose

ISYmu1 CHLl1 gRNA6 TRV2 Architecture_B

Depositor

Steven Jacobsen

Article

Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

Insert

ISYmu1 TnpB

Expression

Plant

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pTW2153

Plasmid

#236577

Purpose

ISYmu1 CHLl1 gRNA3 TRV2 Architecture_B

Depositor

Steven Jacobsen

Article

Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

Insert

ISYmu1 TnpB

Expression

Plant

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pTW2159

Plasmid

#236582

Purpose

ISYmu1 CHLl1 gRNA9 TRV2 Architecture_B

Depositor

Steven Jacobsen

Article

Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

Insert

ISYmu1 TnpB

Expression

Plant

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pTW2155

Plasmid

#236579

Purpose

ISYmu1 CHLl1 gRNA5 TRV2 Architecture_B

Depositor

Steven Jacobsen

Article

Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

Insert

ISYmu1 TnpB

Expression

Plant

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pHEE901-A3A

Plasmid

#229044

Purpose

nCas9 mediated-base editing with NGG PAM recognition motif in Arabidopsis. A3A-nCas9-UGI includes D10A in Cas9.

Depositor

Huawei Zhang

Article

Wang et al Genome Biol. 2024 Aug 9;25(1):215. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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