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Showing: 7501 - 7520 of 8424 results

  2. KK601: pMVP (L3-L2) ecDHFR DD + polyA

    Plasmid
    #121790
    Purpose
    pMVP L3-L2 entry plasmid, contains ecDHFR DD + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term ecDHFR degron domain (stabilized by TMP) to gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    ecDHFR Degron Domain + polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121790-plasmid-map-sequence-id-237291

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  4. pRB117.2

    Plasmid
    #181949
    Purpose
    aTc-inducible expression of NarL with N-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuS
    Depositor
    Jeffrey Tabor
    Article
    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11
    Insert
    mNG-NarL; mCherry; tetR (narL, AX04_RS23485 Synthetic, narL - E. coli)
    Tags
    mNeonGreen
    Expression
    Bacterial
    Mutation
    See Depositor Comments Below
    Promoter
    mNG-NarL-PLtetO-1; mCherry-PdcuS; tetR-J23106
    Available Since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    181949-plasmid-map-sequence-id-356570

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  10. pYPQ166-iSpyMac

    Plasmid
    #149553
    Purpose
    Gateway entry clone for CRISPR-iSpyMacCas9 genome editing at NAAR PAMs
    Depositor
    Yiping Qi
    Article
    Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d
    Insert
    iSpyMacCas9
    Use
    CRISPR; Gateway compatible zispymaccas9 entry clo…
    Tags
    3X FLAG, NLS and NLS
    Expression
    Plant
    Mutation
    R221K, N394K, Protospacer interacting domain form…
    Available Since
    July 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    149553-plasmid-map-sequence-id-306139

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  14. KK501: pMVP (L3-L2) FKBP DD + polyA

    Plasmid
    #121789
    Purpose
    pMVP L3-L2 entry plasmid, contains FKBP DD + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term FKBP degron domain (stabilized by SHLD1) to gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    FKBP Degron Domain + polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121789-plasmid-map-sequence-id-237290

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  20. PB-TAC-OKMS-Cas9

    Plasmid
    #120353
    Purpose
    piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.
    Depositor
    Knut Woltjen
    Article
    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22
    Insert
    OKMS cassette
    Use
    CRISPR; Piggybac transposon
    Expression
    Mammalian
    Mutation
    Klf4 [10-483]
    Available Since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    120353-plasmid-map-sequence-id-236054

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Showing: 7501 - 7520 of 8424 results