We narrowed to 8,532 results for: gnal
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Plasmid#188068PurposeFuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.14)DepositorInsertFuncLib-designed high-redox potential laccase from Trametes hirsuta
TagsS. cerevisiae evolved α-factor prepro-leader sign…ExpressionYeastMutation20 PROSS + 4 FuncLib mutations, described in publ…Available SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
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nERb446-gs10-NLS-VPR
Plasmid#188263PurposePlasmid ensures constitutive expression of the N-terminal fragment of split ERbeta (aa 261-446), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminusDepositorInsertnERb446 (ESR1 Human)
ExpressionMammalianAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLex_Cas9
Plasmid#117987PurposepLex_Cas9 is a single insert lentiviral vector expressing Cas9 driven by CMV promoter.DepositorInsertCas9-P2A-NLS-BASTR
UseLentiviralTagsP2A linker, nuclear localization signal and blast…Available SinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
RXFP1-DuET
Plasmid#213373PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
FPR3-DuET
Plasmid#213241PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
3_T7-mScarlet3-KRas6R
Plasmid#225930PurposeIn vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the K-Ras6R membrane localisation signalDepositorInsertmScarlet3-KRas6R
UseIn vitro transcriptionPromoterT7Available SinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
10_T7-SP-CD8tm-mScarlet3
Plasmid#225937PurposeIn vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with a signal peptide and the CD8 transmembrane domainDepositorInsertSP-CD8tm-mScarlet3
UseIn vitro transcriptionPromoterT7Available SinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENeGFP PCNAL2 pc653
Plasmid#167564PurposeThe GFP-PCNAL2 fusion protein contains a SV40 nuclear localization signal at the NH2 terminus followed by an enhanced mutant GFP gene that is fused to the human PCNA by a linker.DepositorAvailable SinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pK098.CAG-loxP-stop-loxP-nlstagRFP-ires-tTA-WPRE (Supernova)
Plasmid#85179PurposeFor nucleus-specific RFP labeling in single cells, pK098 should be used with pK031.DepositorInsertnuclear localization signal (nls) tagRFP
ExpressionMammalianAvailable SinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_PfeAQ482A
Plasmid#128945Purposeexpresses Q482A mutant of PfeA in E. coliDepositorInsertferric enterobactin receptor
TagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-75), …PromoterT7Available SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-FAS-NES-jRGECO1a-WPRE
Plasmid#141236PurposeAAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of jRGECO1a (jRGECO1a will not be expressed in mammalian cells that express Cre recombinase)DepositorInsertNES-jRGECO1a
UseAAV and Cre/Lox; Cre-offTags6xHIS tag and nuclear export signalPromoterhuman elongation factor-1 alpha (EF-1 alpha)Available SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEX-CMV-SP-YFP-STM2(15-746) EF hand D80A
Plasmid#18864DepositorInsertStromal interaction molecule 2 (STIM2 Human)
TagsYFPExpressionMammalianMutationEF hand mutant: D80A. YFP inserted between signal…Available SinceMarch 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-LmoCas6
Plasmid#126485PurposeEncodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoterDepositorInsertLmoCas6
UseCRISPRTagsFlag, NLSExpressionMammalianPromoterCMV and U6Available SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSUC2::GNSI (NrsR)
Plasmid#121537PurposeThis plasmid contains a CYC1pr driven GNSI reporter and flanking homology to SUC2. Digestion with NotI, SacI, and EcoRV, allows integration at the SUC2 locus.DepositorInsertsCYC1 Promoter (CYC1 Budding Yeast)
Nyv1 Cytosolic Domain (aa 6-230)
Snc1 Transmembrane Domain (TMD)
SUC2 CDS
SUC2 terminator
NrsR
TagsmGFP5ExpressionYeastMutationM109I (Naturally occurring SNP) and The first 21 …Available SinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSP10-bio
Plasmid#47719PurposeExpresses enzymatically monobiotinylated full-length MSP10 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised MSP10
Tagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable SinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ΔN-RNMT
Plasmid#112708PurposeExpresses FLAG-NES-RNMT 121-476 D203A ("ΔN-RNMT") in mammalian cells. Overexpression inhibits cytoplasmic cap methylation.DepositorInsertRNMT (RNMT Human)
TagsFLAG and HIV Rev nuclear export signal (NES)ExpressionMammalianMutationdeleted amino acids 2-120, mutated active-site D2…PromoterCMVAvailable SinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-LmoCas8b2
Plasmid#126482PurposeEncodes L.monocytogenes CRISPR Type I-B Cas8b2 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoterDepositorInsertLmoCas8b2
UseCRISPRTagsFlag, NLSExpressionMammalianPromoterCMV and U6Available SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-LmoCas7
Plasmid#126483PurposeEncodes L.monocytogenes CRISPR Type I-B Cas7 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoterDepositorInsertLmoCas7
UseCRISPRTagsFlag, NLSExpressionMammalianPromoterCMV and U6Available SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-LmoCas5
Plasmid#126484PurposeEncodes L.monocytogenes CRISPR Type I-B Cas5 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoterDepositorInsertLmoCas5
UseCRISPRTagsFlag, NLSExpressionMammalianPromoterCMV and U6Available SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only