We narrowed to 18,339 results for: Mut
-
Plasmid#159752PurposeCRISPR/Cas9 genome editing in plants; a template for PCR-derived fragments used in the assembly of polycistronic transcripts, where sgRNAs are separated by an Arabidopsis alanine tRNA.DepositorArticleInsertsgRNA
UseCRISPRTagsExpressionMutationPromoterAvailable SinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYEE
Plasmid#159750PurposeEgg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by YFP fluoresce.DepositorArticleInsertgRNA scaffold
UseCRISPRTagsExpressionPlantMutationPromoterAvailable SinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYUU
Plasmid#159751PurposeUbiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by YFP fluoresce.DepositorArticleInsertgRNA scaffold
UseCRISPRTagsExpressionPlantMutationPromoterAvailable SinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-SARS2-S-G614 (C-flag)
Plasmid#156421PurposeMammalian expression of SARS-CoV-2 S protein (G614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.DepositorInsertSARS-CoV-2 S protein (S SARS CoV2)
UseTagsFlagExpressionMammalianMutationD614GPromoterCMVAvailable SinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-MRPS31
Plasmid#116764PurposeLentiviral expression of MRPS31DepositorAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-SMAD4
Plasmid#116791PurposeLentiviral expression of SMAD4DepositorAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-STK19
Plasmid#116795PurposeLentiviral expression of STK19DepositorAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NMHC-IIA-S1916A
Plasmid#101040PurposeExpresses GFP-MYH9 construct (human myosin IIA) carrying mutation of serine 1916 to alanine, driven by CMV promoter. Thus inhibits PKC phosphorylation of the tail. Also carries S1915A mutation.DepositorInsertNon-muscle myosin IIA heavy chain (MYH9 Human)
UseTagsGFPExpressionMammalianMutationS1916A, S1917APromoterCMVAvailable SinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-WDR12
Plasmid#116803PurposeLentiviral expression of WDR12DepositorAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-STK11-G56W
Plasmid#116687PurposeLentiviral expression of STK11 G56WDepositorAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-RCAN2
Plasmid#116786PurposeLentiviral expression of RCAN2DepositorAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti TMEM30A
Plasmid#171789Purposetransfer plasmid for lentiviral vector production expressing TMEM30ADepositorAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF2α P405A/P531A-pBabe-Hygro
Plasmid#21676DepositorInsertHypoxia inducible factor 2 alpha (EPAS1 Human)
UseRetroviralTagsExpressionMammalianMutationcDNA changed amino acids from wt: Proline 405 t…PromoterAvailable SinceSept. 4, 2009AvailabilityAcademic Institutions and Nonprofits only -
TP63-T2A-Rox-EF1a-Rox-Venus-NLS repair template
Plasmid#167971PurposeTp63 reporter targeting repair templateDepositorInsertTp63 (TP63 Human)
UseCRISPR; Donor templateTagsVenusExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable SinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR FL CPAP RNAi resistant
Plasmid#46390DepositorInsertCPAP RNAi resistant mutant (CENPJ Human)
UseEntry vectorTagsExpressionMutationsilent mutation that renders it resistant to siRN…PromoterAvailable SinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLU-CMV-Flag-hATG5 WT
Plasmid#111803PurposeMammalian Expression of hATG5DepositorAvailable SinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
AttB_ACE2[dEcto]_IRES-mCherry-H2A-P2A-PuroR
Plasmid#171596PurposeEctodomain deleted negative control ACE2 from Bxb1 landing padDepositorInsertsUseSynthetic Biology; Promoterless bxb1 dna recombin…TagsExpressionMammalianMutationPromoterAvailable SinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1-ISG15 C78SN13Y
Plasmid#165106Purposebacterial expressio of a GST fusion of Human ISG15 C78SN13YDepositorInsertISG15 (ISG15 Human)
UseTagsGST from plasmid.ExpressionBacterialMutationC78SN13Y; The sequence of ISG15-C78S/N13Y contai…PromoterAvailable SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRC/CMV-TYK2V678F-VSV
Plasmid#139349PurposeExpression of a gain of function TYK2-V678F protein (correspoding to JAK2-V617F)DepositorInsertTYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
UseTagsVSVExpressionMammalianMutationV362F-V678FPromoterCMVAvailable SinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
CRY2-INPP5E
Plasmid#79562Purposeexpression of CRY2PHR fused to inositol 5-phosphatase domain of human INPP5E with mutated C-terminal CaaX-motifDepositorInsertINPP5E (INPP5E Human)
UseTagsCRY2 (photolyase homology region domain of Arabid…ExpressionMammalianMutationC-terminal region, aa 214_644, C641A mutation to …PromoterCMVAvailable SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only