We narrowed to 16,201 results for: genome

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pCas9-NT

Plasmid

#235165

Purpose

Expresses the codon harmonized Streptococcus pyogenes cas9 gene and a NT sgRNA

Depositor

Constantinos Patinios

Article

Della Valle et al Trends Biotechnol. 2025 Jun;43(6):1470-1

Insert

SpCas9

Expression

Bacterial

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCas12a-NT

Plasmid

#235167

Purpose

Expresses the Wild Type Francisella novicida cas12a gene and a NT crRNA

Depositor

Constantinos Patinios

Article

Della Valle et al Trends Biotechnol. 2025 Jun;43(6):1470-1

Insert

FnCas12a

Expression

Bacterial

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Kir2.1WT-2A-mScarlet-KASH

Plasmid

#203840

Purpose

Kir2.1 expression for silencing neurons and mScarlet-KASH expression

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Has Service

AAV2 and AAV8

Insert

Kir2.1 (Kcnj2 Mouse)

Use

AAV

Tags

2A-mScarlet-KASH

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pORANGE unc13a GFP KI

Plasmid

#131498

Purpose

Endogenous tagging of Munc13-1: C-terminal (amino acid position: A1762)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Unc13a Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pT3-EF1a-c-Met

Plasmid

#31784

Depositor

Xin Chen

Article

Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

Insert

Met (MET Human)

Expression

Mammalian

Promoter

EF1alpha

Available Since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only

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PRDM9(KRAB-SSXRD-PR/SET-ZF1-dC)-Cas9

Plasmid

#184464

Purpose

Plasmid encoding PRDM9-Cas9 fusion under CMV promoter

Depositor

Jennifer Doudna

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

Insert

PRDM9(KRAB-SSXRD-PR/SET-ZF1-dC)-Cas9 (PRDM9 Human)

Use

CRISPR

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pX458-ECFP

Plasmid

#112220

Purpose

Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and ECFP

Depositor

Yinming Liang

Article

Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

Insert

ECFP

Use

CRISPR

Expression

Mammalian

Available Since

July 11, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCV(W-)U6sgRNA5(BbsI)-PGKpuroBFP

Plasmid

#102797

Purpose

Retrovirus for testing CRISPR KO activity (empty) - non-targeting control plasmid contains GFP instead of PuroR

Depositor

Sarah Teichmann

Article

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Inserts

sgRNA
EGFP

Use

CRISPR and Retroviral

Expression

Mammalian

Mutation

H231L

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2

Plasmid

#74009

Purpose

Tissue-specific knock-out in zebrafish, Cas9 and GFP driven by a UAS promoter

Depositor

Filippo Del Bene

Article

Di Donato et al Genome Res. 2016 Mar 8.

Inserts

5x UAS
Cas9
T2A
GFP
U6 promoter

Use

CRISPR

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-TadA8e-PcrA M6

Plasmid

#221229

Purpose

Express HACE Editor as a fusion of TadA-8e adenosine deaminase and an optimized PcrA M6 helicase.

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

TadA8e-PcrA M6

Expression

Mammalian

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pSL1425 (pSPIN, pCDF backbone)

Plasmid

#160735

Purpose

Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA and has BsaI sites for spacer cloning. pCDF backbone.

Depositor

Samuel H. Sternberg

Article

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST proteins, crRNA, and donor DNA

Expression

Bacterial

Promoter

J23119

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pS-sg:GFP

Plasmid

#196296

Purpose

35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding sgRNA:GFP fusion in place of viral NSs

Depositor

Zhenghe Li

Article

Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

Insert

Full length TSWV S antigenome encoding sgRNA:GFP fusion in place of viral NSs

Use

CRISPR

Expression

Plant

Promoter

duplicated cauliflower mosaic virus 35S promoter

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5 BRGI-Flag

Plasmid

#19143

Depositor

Joan Massague

Article

Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

Insert

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)

Tags

Flag

Expression

Mammalian

Available Since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only

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pM-Cas9

Plasmid

#196325

Purpose

35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding SpCas9 in place of viral GP

Depositor

Zhenghe Li

Article

Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

Insert

Full length TSWV M antigenome encoding SpCas9 in place of viral GP

Use

CRISPR

Tags

3×flag

Expression

Plant

Mutation

See depositor comments below

Promoter

duplicated cauliflower mosaic virus 35S promoter

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pLR5-CBh-dCas9-hUtx-IRES-Hyg

Plasmid

#122374

Purpose

Expresses dCas9-hUtx-IRES-Hyg in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

CBh-dCas9-hUtx-IRES-Hyg-pA

Use

Piggybac

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pET-Cas9-6xHis

Plasmid

#62374

Purpose

Expression of Cas9-6xHis in bacterial cells

Depositor

David Liu

Article

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

Cas9_6xHis

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

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pIND(V5)EcoDam

Plasmid

#59201

Purpose

Mammalian DamID vector used to fuse gene of interest to V5-tagged E.coli Dam. Can also express Dam alone as negative control.

Depositor

Bas van Steensel

Article

Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

Type

Empty backbone

Use

Damid

Tags

Dam (DNA adenine methyltransferase) and V5

Expression

Mammalian

Promoter

Heat Shock Minimal Promoter

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pTW2154

Plasmid

#236578

Purpose

ISYmu1 CHLl1 gRNA4 TRV2 Architecture_B

Depositor

Steven Jacobsen

Article

Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

Insert

ISYmu1 TnpB

Expression

Plant

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pQTEV-ANP32A

Plasmid

#31563

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

acidic (leucine-rich) nuclear phosphoprotein 32 family, member A (ANP32A Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

Plasmid

#42229

Purpose

This plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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