We narrowed to 15,587 results for: Ott

1. pFUSE-B_P2rP4

   Plasmid

   #97185

   Purpose

   For fusion gene cloning. Recombine Gene B into this Gateway Entry Vector

   Depositor

   Kenneth Scott

   Article

   Lu et al Cancer Res. 2017 May 16. pii: canres.274

   Type

   Empty backbone

   Use

   Gateway

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRB20

   Plasmid

   #52559

   Purpose

   serves as PCR-template to generate HR donors for C-terminal YFP-tag

   Depositor

   Klaus Foerstemann

   Article

   Bottcher et al Nucleic Acids Res. 2014 Apr 19.

   Insert

   eYFP and Blasticigin resistance cassette
   

   Use

   Blunt cloning kit

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMC8b-XI3up

   Plasmid

   #176170

   Purpose

   yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-3

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Insert

   5' homology for integration site XI-3
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMC7-XI3dw

   Plasmid

   #176171

   Purpose

   yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-3

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Insert

   3' homology for integration site XI-3
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMC8b-XI5up

   Plasmid

   #176172

   Purpose

   yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-5

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Insert

   5' homology for integration site XI-5
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMC7-XI5dw

   Plasmid

   #176173

   Purpose

   yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-5

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Insert

   3' homology for integration site XI-5
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMC8b-XII5up

   Plasmid

   #176176

   Purpose

   yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XII-5

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Insert

   5' homology for integration site XII-5
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMC7-XII5dw

   Plasmid

   #176177

   Purpose

   yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XII-5

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Insert

   3' homology for integration site XII-5
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMC8b-X2up

   Plasmid

   #176162

   Purpose

   yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site X-2

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Insert

   5' homology for integration site X-2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMC7-X2dw

    Plasmid

    #176163

    Purpose

    yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site X-2

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    3' homology for integration site X-2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUASp YFP Rab3 WT

    Plasmid

    #37687

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab3 (Rab3 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    Promoter

    GAL4

    Available since

    Nov. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pUASp YFP Rab10 WT

    Plasmid

    #37708

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab10 (Rab10 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    Promoter

    GAL4

    Available since

    Nov. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pUASp YFP Rab10 DN

    Plasmid

    #37709

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab10 (Rab10 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    T23N (dominant negative)

    Promoter

    GAL4

    Available since

    Nov. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pME-BTK-PH

    Plasmid

    #67673

    Purpose

    Gateway entry clone containing BTK-PH with no stop codon

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    BTK-PH-NS
    

    Use

    Gateway multisite middle entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUASp YFP Rab10 CA

    Plasmid

    #37710

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab10 (Rab10 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    Q68L (constitutive active)

    Promoter

    GAL4

    Available since

    Nov. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pUASP YFP Rab7 WT

    Plasmid

    #37699

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab7 (Rab7 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    Promoter

    GAL4

    Available since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

17. Cav1-miniSOG

    Plasmid

    #67667

    Purpose

    Mammalian expression vector driving dog Caveolin1-minSOG

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    Dog Cav1-miniSOG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUASp YFP Rab8 CA

    Plasmid

    #37707

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab8 (Rab8 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    Q67L (constitutive active)

    Promoter

    GAL4

    Available since

    Nov. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pUASp YFP Rab8 DN

    Plasmid

    #37706

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab8 (Rab8 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    T22N (dominant negative)

    Promoter

    GAL4

    Available since

    Nov. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pUASp YFP Rab30 CA

    Plasmid

    #37737

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab30 (Rab30 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    Q66L (constitutive active)

    Promoter

    GAL4

    Available since

    Nov. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

21. pUASP dsRed Rab10 WT

    Plasmid

    #44828

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab10 (Rab10 Fly)
    

    Use

    Drosophila

    Tags

    dsRed

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 18, 2013

    Availability

    Academic Institutions and Nonprofits only

22. pUASP YFP Rab18 WT

    Plasmid

    #37717

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab18 (Rab18 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    Promoter

    GAL4

    Available since

    March 13, 2013

    Availability

    Academic Institutions and Nonprofits only

23. pKF251

    Plasmid

    #52532

    Purpose

    Double-GFP reporter for DNA cleavage

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    Double-GFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    ubiquitin

    Available since

    April 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pUAST td tomato Rab35 CA

    Plasmid

    #58383

    Purpose

    expression of drosophila tdtomato-Rab35 constitutively active mutant

    Depositor

    Matthew Scott

    Article

    Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

    Insert

    Rab35 (Rab35 Fly)
    

    Use

    Tags

    td tomato

    Expression

    Insect

    Mutation

    Q67L (constitutive active)

    Promoter

    GAL4

    Available since

    July 21, 2014

    Availability

    Academic Institutions and Nonprofits only

25. pFasBac+GFP-CAMSAP2 CC

    Plasmid

    #59040

    Purpose

    for baculovirus expression of CAMSAP2 CC in Sf9 cells

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    CAMSAP2 CC domain (CAMSAP2 Human)
    

    Use

    Tags

    His and eGFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pUASP YFP Rab8 WT

    Plasmid

    #37705

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab8 (Rab8 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    Promoter

    GAL4

    Available since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

27. pUAST YFP Rab2 WT

    Plasmid

    #58444

    Purpose

    expression of drosophila YFP-Rab2

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab2 (Rab2 Fly)
    

    Use

    Tags

    YFP

    Expression

    Insect

    Mutation

    Promoter

    GAL4

    Available since

    July 21, 2014

    Availability

    Academic Institutions and Nonprofits only

28. pUASp YFP Rab6 WT

    Plasmid

    #37696

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab6 (Rab6 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    Promoter

    GAL4

    Available since

    Nov. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

29. pCMV-EPN-01*(ΔL1)

    Plasmid

    #80042

    Purpose

    Expression of PTAP L domain mutant EPN-01*

    Depositor

    Wesley Sundquist

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    pCMV-EPN-01*(ΔL2)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    PTAP sequence in p6 mutated to Ala

    Promoter

    CMV

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET-21a-GFP-Patronin CC 1288–1457

    Plasmid

    #59055

    Purpose

    for E. coli expression of Patronin CC truncation

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    Patronin (Patronin Fly)
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pFasBac+GFP-CAMSAP3 CC

    Plasmid

    #59042

    Purpose

    for baculovirus expression of mouse CAMSAP3 CC in Sf9 cells

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    CAMSAP3 CC domain (Camsap3 Mouse)
    

    Use

    Tags

    His and eGFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pUASp YFP Rab18 DN

    Plasmid

    #53474

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab18 (Rab18 Fly)
    

    Use

    Tags

    YFP

    Expression

    Insect

    Mutation

    S19N

    Promoter

    GAL4

    Available since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

33. pUASp YFP Rab18 CA

    Plasmid

    #37719

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab18 (Rab18 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    A64L (constitutive active)

    Promoter

    GAL4

    Available since

    March 13, 2013

    Availability

    Academic Institutions and Nonprofits only

34. p201N 1514

    Plasmid

    #47025

    Purpose

    Contains soybean miRNA miR1514 recognition sequence to produce siRNAs from 3' target sequences and induce RNA silencing

    Depositor

    Wayne Parrott

    Article

    Jacobs et al Plant Biotechnol J. 2015 Mar 27. doi: 10

    Inserts

    gma-miR1514 recognition sequence
    nptII selectable marker
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    GmUbi and StUbi-3

    Available since

    Aug. 30, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. pUAST YFP Rab18 DN

    Plasmid

    #58722

    Purpose

    expression of drosophila YFP Rab18 dominant negative mutant

    Depositor

    Matthew Scott

    Article

    Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

    Insert

    Rab18 (Rab18 Fly)
    

    Use

    Tags

    YFP

    Expression

    Insect

    Mutation

    S19N

    Promoter

    GAL4

    Available since

    Sept. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

36. pUASp YFP Rab1 WT

    Plasmid

    #37681

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab1 (Rab1 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    Promoter

    GAL4

    Available since

    Nov. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

37. pUAST YFP Rab21 WT

    Plasmid

    #58455

    Purpose

    expression of drosophila YFP Rab21

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab21 (Rab21 Fly)
    

    Use

    Tags

    YFP

    Expression

    Insect

    Mutation

    Promoter

    GAL4

    Available since

    July 23, 2014

    Availability

    Academic Institutions and Nonprofits only

38. pUASP dsRed Rab3 WT

    Plasmid

    #44820

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab3 (Rab3 Fly)
    

    Use

    Drosophila

    Tags

    dsRed

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 17, 2013

    Availability

    Academic Institutions and Nonprofits only

39. pUASp YFP Rab14 WT

    Plasmid

    #37714

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab14 (Rab14 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    Promoter

    GAL4

    Available since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

40. pUASp YFP Rab35 CA

    Plasmid

    #37743

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab35 (Rab35 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    Q67L (constitutive active)

    Promoter

    GAL4

    Available since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

41. pUASp YFP Rab30 DN

    Plasmid

    #37736

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab30 (Rab30 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    T21N (dominant negative)

    Promoter

    GAL4

    Available since

    Nov. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

42. pGL2084

    Plasmid

    #137726

    Purpose

    Gateway vector for inducible stem loop RNAi in T.b. brucei 2T1

    Depositor

    Jeremy Mottram

    Article

    Jones et al PLoS Pathog. 2014 Jan;10(1):e1003886. do

    Type

    Empty backbone

    Use

    RNAi; Trypanosoma brucei tet inducible stem loop

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCRISPR3_BC2

    Plasmid

    #191857

    Purpose

    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: ATCATG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

    Depositor

    Kimberly Reynolds

    Article

    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCRISPR3_BC6

    Plasmid

    #191861

    Purpose

    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CCTAGT) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

    Depositor

    Kimberly Reynolds

    Article

    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pCRISPR3_BC5

    Plasmid

    #191860

    Purpose

    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: AGTCTA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

    Depositor

    Kimberly Reynolds

    Article

    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pCRISPR3_BC4

    Plasmid

    #191859

    Purpose

    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GTATGA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

    Depositor

    Kimberly Reynolds

    Article

    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pCRISPR3_BC3

    Plasmid

    #191858

    Purpose

    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GCATGG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

    Depositor

    Kimberly Reynolds

    Article

    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pCRISPR3_BC1

    Plasmid

    #191856

    Purpose

    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CTTTCA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

    Depositor

    Kimberly Reynolds

    Article

    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pMCL8

    Plasmid

    #176184

    Purpose

    gRNA cloning cassette with RPR1p-tetO promoter and terminator RPT1t, RPR1p-tetO-GFP-scRNA-RPR1t

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pMCL8_28bp

    Plasmid

    #176185

    Purpose

    plasmid containing scaffold gRNA for Cas9 followed by a 28bp Csy4 recognition sequence, RPR1p-tetO-GFP-scRNA-28bpCsy4-RPR1t

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge