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We narrowed to 7,871 results for: 199

Showing: 741 - 760 of 7871 results

  5. AP70_pU.CAG.Cas9-D10A-K848A-R1060A.rBGpA

    Plasmid
    #199254
    Purpose
    Expression construct encoding the high specificity SpCas9-KARA-D10A nickase
    Depositor
    Manuel Goncalves
    Article
    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117
    Insert
    High specificity S. pyogenes Cas9-D10A-KARA nickase
    Use
    CRISPR
    Tags
    3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A K848A R1060A
    Promoter
    CAG promoter
    Available since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199254-plasmid-map-sequence-id-391656

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  11. pSiM2

    Plasmid
    #199381
    Purpose
    Encodes dual cistronic membrane localized GCaMP6f and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    GCaMP6f::ras-2CAAX::SL2::mScarlet-I::ras-2CAAX
    Use
    Tags
    Expression
    Bacterial
    Mutation
    no mutations, except added subcellular localizati…
    Promoter
    Available since
    April 24, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    199381-plasmid-map-sequence-id-391045

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  14. CSTI.pJL1

    Plasmid
    #199113
    Purpose
    In vitro expression of glycosyltransferase from Campylobacter jejuni for elaboration of sialic acid onto galactose in α2,3 linkage using CMP-sialic acid as substrate
    Depositor
    Michael Jewett
    Article
    Thames et al ACS Synth Biol. 2023 Apr 11. doi: 10.102
    Insert
    CSTI
    Use
    Tags
    CAT linker, Strep-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    199113-plasmid-map-sequence-id-390798

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  15. NeuA.pJL1

    Plasmid
    #199115
    Purpose
    In vitro expression of N-acylneuraminate cytodiylyltransfease (NeuA), which conjugates sialic acid and CTP to make activated sugar donor, CMP-sialic acid (see CSTI.PJL1 and PdST6.PJL1)
    Depositor
    Michael Jewett
    Article
    Thames et al ACS Synth Biol. 2023 Apr 11. doi: 10.102
    Insert
    N-acylneuraminate cytodiylyltransfease (NeuA)
    Use
    Tags
    Strep-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    199115-plasmid-map-sequence-id-390797

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  16. Derp2.pJL1

    Plasmid
    #199116
    Purpose
    In vitro expression of Der p 2, a major common allergen from Dermatophagoides pteronyssinus (dust mite), with synthetic site for glycosylation encoded (GGNWTT)
    Depositor
    Michael Jewett
    Article
    Thames et al ACS Synth Biol. 2023 Apr 11. doi: 10.102
    Insert
    Der p 2 dust mite allergen
    Use
    Tags
    Glyctag, His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    199116-plasmid-map-sequence-id-390794

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  17. pGBT9-GGA2-GAE

    Plasmid
    #199175
    Purpose
    Expression of GAL4 DNA-binding domain (BD)-GGA2-GAE domain fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.
    Insert
    GGA2-GAE domain (GGA2 Human)
    Use
    Tags
    GAL4-DNA binding domain fragment
    Expression
    Yeast
    Mutation
    Contains Lys 545 Gln substitution
    Promoter
    ADH1
    Available since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    199175-plasmid-map-sequence-id-389860

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Showing: 741 - 760 of 7871 results