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pTOPO-MCL1

Plasmid

#21605

Depositor

Ulrich Maurer

Publication

Maurer et al Mol Cell. 2006 Mar 17. 21(6):749-60.

Insert

MCL-1 (MCL1 Human)

Expression

Mammalian

Mutation

Insert ends with a STOP codon, no V5-His-tag.

Available since

Aug. 11, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles
pCMV:GFP(loxP)lacZ

Plasmid

#31125

Depositor

Gerhart Ryffel

Publication

Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

Insert

GFP(loxP)lacZ

Use

Cre/Lox

Expression

Mammalian

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Mfn1(K88T)-10xmyc

Plasmid

#26050

Depositor

David Chan

Publication

Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

Insert

Mfn1 (Mfn1 Mouse)

Tags

10xMyc

Expression

Mammalian

Mutation

K88T GTPase mutant

Available since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pCDH-CB-IRES-copGFP-T2A-Puro

Plasmid

#72299

Purpose

Express gene of interest under CB promoter

Depositor

Kazuhiro Oka

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

chicken beta-actin + CMV enhancer

Available since

Jan. 25, 2016

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

5 Articles

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nAChR alpha4 WT

Plasmid

#24271

Depositor

Henry Lester

Publication

Nashmi et al J Neurosci. 2003 Dec 17. 23(37):11554-67

Insert

nAChR alpha4 WT (Chrna4 Mouse)

Expression

Mammalian

Available since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
LM627

Plasmid

#191551

Purpose

LM627_MSG-AGGA-promoter-ccdb-TTCC-SNAC-his

Depositor

David Baker

Publication

Wicky et al Science. 2022 Oct 7;378(6615):56-61. doi

Insert

ccdb

Tags

SNAC-tag, HIS-tag

Expression

Bacterial

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pSEMS-Tom20-Halo7Tag

Plasmid

#111135

Purpose

Tom20 with selflabeling tag, e.g. for fluorescence microscopy

Depositor

Karin Busch

Publication

Appelhans et al Nano Lett. 2012 Feb 8;12(2):610-6. doi:

Insert

Receptor of tranlocase of outer mitochondrial membrane Tom20 (TOMM20 Human)

Tags

Halo7 Tag

Expression

Mammalian

Promoter

CMV

Available since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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GFP-mouse vinculin full length (889)

Plasmid

#67935

Purpose

Mammalian expression of mouse vinculin with GFP fusion

Depositor

Alpha Yap

Publication

Leerberg et al Curr Biol. 2014 Aug 4;24(15):1689-99. do

Insert

Vinculin (Vcl Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

L159P in vinculin

Promoter

CMV

Available since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pEGFP-Akt2

Plasmid

#86593

Purpose

Expresses eGFP-tagged human Akt2(WT)

Depositor

Thomas Leonard, Ivan Yudushkin

Publication

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt2 (AKT2 Human)

Expression

Mammalian

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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FU- dio Teal-gephyrin - W

Plasmid

#73918

Purpose

To express the inhibitory scaffold gephyrin tagged to teal fluorescent protein. Expression is Cre dependant. Plasmid can also be used to make lentivirus.

Depositor

Elly Nedivi

Publication

Villa et al Neuron. 2016 Feb 3. pii: S0896-6273(16)0

Insert

Teal-gephyrin

Use

Lentiviral

Tags

Teal

Expression

Mammalian

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pRSET his-eGFP

Plasmid

#113551

Purpose

For bacterial expression and purification of eGFP

Depositor

Jeanne Stachowiak

Publication

Busch et al Nat Commun. 2015 Jul 24;6:7875. doi: 10.

Insert

his-eGFP

Expression

Bacterial

Mutation

A206K

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA3-Flag-NSF

Plasmid

#74924

Purpose

Mammalian expression of human NSF full-length (744 aa)

Depositor

Elisa Greggio

Publication

Belluzzi et al Mol Neurodegener. 2016 Jan 13;11(1):1. d

Insert

NSF (NSF Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Cytosolic FAK biosensor

Plasmid

#78300

Purpose

FRET biosensor. Visualization of cytosolic FAK activity in live cells

Depositor

Yingxiao Wang

Publication

Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

Insert

Cytosolic FAK biosensor

Expression

Mammalian

Promoter

CMV

Available since

June 10, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pHR-CMV-TetO2_3C-Twin-Strep_IRES-EmGFP

Plasmid

#113884

Purpose

Mammalian (inducible) protein expression, bicistronic expression of cytosolic EmGFP

Depositor

A. Radu Aricescu

Publication

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Twin-Strep

Promoter

pHR-CMV-TetO2

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pirespuro2-CTLA4-mEos2

Plasmid

#98285

Purpose

mammalian expression of CTLA4-mEos2

Depositor

Mike Heilemann

Publication

Fricke et al Sci Rep. 2015 Sep 11;5:14072. doi: 10.10

Insert

CTLA4 (CTLA4 Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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PCMV-intron myc Rab2 S20N

Plasmid

#46780

Purpose

expression of S20N Rab2a in mammalian cells

Depositor

Terry Hébert

Publication

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

RAB2 (RAB2A Human)

Tags

myc

Expression

Mammalian

Mutation

S20N dominant negative

Promoter

CMV

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
AAV_pMBP-EGFP-caax

Plasmid

#190155

Purpose

Expresses membrane-targeted EGFP in oligodendrocytes; AAV vector

Depositor

Brad Zuchero

Publication

Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

Has service

AAV1 and AAV8

Insert

EGFP-caax

Use

AAV

Expression

Mammalian

Promoter

MBP

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGCaMP6m-N3-TPC2

Plasmid

#80147

Purpose

GCaMP6 is a genetically encoded fluorescent Ca2+ sensor . TPC2-GCaMP6 allows the visualization of Ca2+ release from intracellular Ca2+ stores in real time

Depositor

Santiago Di Pietro

Publication

Ambrosio et al Mol Biol Cell. 2015 Sep 15;26(18):3263-7

Insert

TPCN2 (TPCN2 Human)

Tags

GCaMP6m

Expression

Mammalian

Promoter

CMV

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pT3TS-RfxCas13d-NLS-HA

Plasmid

#141321

Purpose

Plasmid to carry out IVT of RfxCas13d-NLS (human codon-optimized)

Depositor

Ariel Bazzini, Miguel Angel Moreno-Mateos

Publication

Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

Insert

Rfx-Cas13d (NLS-RfxCas13d-NLS)

Use

CRISPR

Tags

HA and NLS

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3 PARL-FLAG-CT wild type

Plasmid

#13639

Depositor

Luca Pellegrini

Publication

Sik et al J Biol Chem. 2004 Apr 9. 279(15):15323-9

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Tags

FLAG

Expression

Mammalian

Available since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles