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pCellFree_G03 FYN

Plasmid

#67106

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

FYN (FYN Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pJJH1338

Plasmid

#36912

Depositor

Jürgen Heinisch

Article

Bakota et al Mol Genet Genomics. 2012 Apr;287(4):313-

Insert

ScURA3

Use

Lentiviral

Expression

Bacterial, Mammalian, and Y…

Available Since

July 17, 2012

Availability

Academic Institutions and Nonprofits only

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pEMS1369

Plasmid

#29170

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple105 (HAP1 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available Since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pEMS1353

Plasmid

#29155

Purpose

Expression of the eGFP reporter gene was not detected in the brain and eye.

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple137 (NR2E1 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available Since

Nov. 7, 2011

Availability

Academic Institutions and Nonprofits only

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SNAP-tag TDP-43

Plasmid

#237552

Purpose

Expression of SNAP-tag fused to wild-type TDP-43

Depositor

Tatyana Shelkovnikova

Article

Hodgson et al Nat Cell Biol. 2026 Mar 18. doi: 10.1038

Insert

SNAP-tagged TDP-43 (TARDBP Human)

Tags

SNAP tag

Expression

Mammalian

Mutation

wild-type TDP-43

Promoter

CMV

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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p150-CC1-tagBFP

Plasmid

#222314

Purpose

Inactivates dynein function.

Depositor

Franck Perez

Article

Fourriere et al J Cell Sci. 2016 Sep 1;129(17):3238-50.

Insert

Coiled-coil domain of p150glued (DCTN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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2xMyc-tdMCP-SNAP (tdMCP standard)

Plasmid

#226166

Purpose

For transient expression of the tdMCP-protein standard. Used in LABEL-seq abundance assays.

Depositor

Dustin Maly

Article

Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

Insert

2xMyc-tdMCP-SNAPtag

Use

Lentiviral

Tags

2xMyc-tdMCP and EGFP-IRES-BlastR

Expression

Mammalian

Available Since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-iGlu3slow1

Plasmid

#226501

Purpose

Superslow-decay iGluSnFR3 variant 1 for population imaging

Depositor

Katalin Torok

Article

Tran et al bioRxiv, August 30th, 2024

Insert

iGluSnFR3 D25A

Tags

IgK, Myc tag, and PDGFR Beta TM domain

Expression

Mammalian

Mutation

D25A

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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PGK+ plasmid with onboard mCherry cassette

Plasmid

#169745

Purpose

PGK+ plasmid that also encodes separate mCherry cassette. mCherry is used to monitor plasmid dosage.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

eGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

PGK-tetO2

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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OSCA1.2-PP-GFP

Plasmid

#135390

Purpose

Expresses OSCA1.2 in mammalian cells

Depositor

Ardem Patapoutian

Article

Jojoa-Cruz et al Elife. 2018 Nov 1;7. pii: 41845. doi: 10

Insert

OSCA1.2 (AT4G22120 Mustard Weed)

Tags

EGFP tag connected to the gene via a precision pr…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pSBL_DK202

Plasmid

#156426

Purpose

Mammalian expression of 3a delta N(aa 42-275)-EGFP with CMV promoter, can be used for transfection or used to generate bacmids for baculoviral infection of mammalian cells

Depositor

Stephen Brohawn

Article

Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

Insert

SARS-CoV-2 3a protein (aa 42-275)

Tags

EGFP-His

Expression

Mammalian

Mutation

aa 42-275

Promoter

CMV

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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151_pAAV-ProC29-CatCh-GFP-WPRE

Plasmid

#125964

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProC29

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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114_pAAV-ProC22-CatCh-GFP-WPRE

Plasmid

#125957

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProC22

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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137_pAAV-ProA3-CatCh-GFP-WPRE

Plasmid

#125887

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProA3

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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37_pAAV-ProC40-CatCh-GFP-WPRE

Plasmid

#125974

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProC40

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pSNAI2-donor

Plasmid

#112378

Purpose

CRISPR donor plasmid to tag human transcription factor SNAI2 with GFP

Depositor

Kevin White

Insert

SNAI2 homology arms flanking EGFP-IRES-Neo cassette (SNAI2 Human)

Use

CRISPR

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pEMS1358

Plasmid

#29302

Purpose

Expression of the eGFP reporter gene was not detected in the brain and eye.

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple162 (PITX3 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available Since

June 10, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FXR1a-sh5Resistant

Plasmid

#225448

Purpose

Express mEGFP-fusion protein of FXR1 isoform a with shRNA5-resistant silent mutation

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FXR1 isoform a (FXR1 Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-iRFP713-ORP9-PH

Plasmid

#214269

Purpose

Mammalian expression of near-IR fluorescent PI4P reporter iRFP713-ORP9-PH

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

iRFP713-ORP9(1-111) (Osbpl9 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-mTagBFP2-ORP9-PH

Plasmid

#214267

Purpose

Mammalian expression of blue fluorescent PI4P reporter mTagBFP2-ORP9-PH

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

mTagBFP2-ORP9(1-111) (Osbpl9 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-RVR-ABE8e-V106W

Plasmid

#193659

Purpose

Expression of base editor RVR-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

RVR-dLbCas12a-TadA*8e-V106W, EGFP

Expression

Mammalian

Mutation

dLbCas12a-G532R/K538V/Y542R, TadA*8e-V106W

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-CAG-GFP-EnvA(N2c)

Plasmid

#194353

Purpose

Lentivirus expressing eGFP and (EnvA-N2c Rabies Glycoprotein) fusion protein. Used to package EnvA pseudotyped G-deleted Rabies of CVS-N2c strain

Depositor

Marco Tripodi

Article

Lee et al Nat Methods. 2023 Apr;20(4):580-589. doi

Inserts

Enhanced Green Fluorescent Protein (eGFP)
EnvA-CVS-N2c Rabies Glycoprotein

Use

Lentiviral

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO-[4xCox8]-ChemoG-NAD

Plasmid

#193823

Purpose

CMV overexpression of ChemoG-NAD in the mitochondria of mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

[4xCox8]-ChemoG-NAD

Expression

Mammalian

Mutation

EGFP:A206K, T225R; HT7: L271E

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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FKBP-AP180-GFP

Plasmid

#186577

Purpose

Expression of the fusion protein FKBP-AP180-GFP. Uses rat AP180 residues 328-896.

Depositor

Stephen Royle

Article

Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

Insert

AP180 (Snap91 Rat)

Tags

FKBP N-terminal GFP C-terminal

Expression

Mammalian

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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p5xQUAS-ZsGreen-P2A, α-cry:mCherry

Plasmid

#180477

Purpose

Inducible gene expression vector p5xQUAS-ZsGreen-P2A, α-cry:mCherry

Depositor

Hyunju Ro

Article

Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

Inserts

5x QUAS-ZsGreen-P2A
mCherry

Use

Synthetic Biology

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA1_SARS-COV-2_ORF7a

Plasmid

#169393

Purpose

Cell-free/mammalian expression vector for SARS-COV-2 virus ORF7a with N-term eGFP

Depositor

Kirill Alexandrov

Insert

SARS-COV-2 _ORF7a (ORF7a SARS-Cov2)

Use

Cell-free expression vector

Tags

His and eGFP

Mutation

Codon optimized for Saccharomyces cerevisiae

Promoter

T7- CMV/CAG

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV AURKA-mTurq2

Plasmid

#157767

Purpose

Expression of AuroraA kinase fused to mTurquoise2 under CMV promoter

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pJOE6090.1

Plasmid

#135080

Purpose

pBR322-based L-rhamnose-inducible vector.

Depositor

Josef Altenbuchner

Article

Hoffmann et al PLoS One. 2015 Jul 24;10(7):e0133248. do

Insert

P-rhaP-BAD-Strep-tag II-eGFP

Expression

Bacterial

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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XR1_pHCLYBL-CAG-LSL-GFP-P2A-RFP

Plasmid

#129111

Purpose

Homologous recombination vector for excision reporter 1 in the CLYBL locus.

Depositor

Bruce Conklin

Insert

LSL, eGFP, NLS-RFP

Use

Cre/Lox and TALEN

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 30, 2019

Availability

Academic Institutions and Nonprofits only

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ptagBFP-LacI-NLS-VP16

Plasmid

#103837

Purpose

Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited lacO arrays

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

tagBFP-LacI-NLS-VP16

Expression

Mammalian

Promoter

SV2

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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