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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 36,352 results for: lat

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Showing: 7581 - 7600 of 36352 results

  1. PGK 4xMito-mEmerald

    Plasmid
    #200430
    Purpose
    Mammalian expression of fluorescently-tagged marker for mitochondria
    Depositor
    Erika Holzbaur
    Publication
    Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    4x cytochrome c oxidase subunit VIII mitochondrial targeting sequence
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    4x mito-targeting sequence
    Promoter
    PGK
    Available since
    May 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    200430-plasmid-map-sequence-id-393638

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  2. pAAV2-CAG-3xNLS-vsfGFP-0

    Plasmid
    #191097
    Purpose
    To express a bright green nanobody FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods
    Depositor
    Murray Blackmore, Pantelis Tsoulfas
    Publication
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    3xNLS-vsfGFP-0
    Use
    AAV
    Tags
    Green fluorescent protein bound to enhancer nanob…
    Expression
    Mammalian
    Mutation
    Optimized to Human codon usage
    Promoter
    CMV and CAG
    Available since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    191097-plasmid-map-sequence-id-380121

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  5. pCC_02 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139087
    Purpose
    Expresses human codon-optimized Cas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    SpCas9-NG
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    L1111R, D1135V,G1218R, E1219F, A1322R, R1335V, T1…
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    139087-plasmid-map-sequence-id-273883

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  7. p-mCherry-C1-Aurora

    Plasmid
    #98167
    Purpose
    red-shifted artificial anion conducting channelrhodopsin (aACR). Activation up to 600 nm; off-kinetics 260 ms. Codon optimized for mammalian expression.
    Depositor
    Peter Hegemann
    Publication
    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Synthetic construct Aurora gene
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    V59S, E83N, E90Q, E101S, V117R, E123S, P242R, A24…
    Promoter
    CMV (+enhancer)
    Available since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98167-plasmid-map-sequence-id-182713

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  11. pcDNAI Neo human PECAM FL

    Plasmid
    #42902
    Depositor
    Steven Albelda
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PECAM (PECAM1 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 20, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  15. pSBtet rtTA G72V SE Δsplice GP Luc

    Plasmid
    #194328
    Purpose
    Tetracycline inducible expression of Luciferase enzyme in mammalian cells with inclusion of rtTA G72V mutation, splice site and high sensitivity mutations for increased promotor tightness
    Depositor
    Alexander McLellan
    Publication
    Saunderson et al Genes (Basel). 2022 Sep 20;13(10). pii:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Luciferase
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    Tet-responsive promotor related mutations: G72V i…
    Promoter
    Tight tet-responsive promotor
    Available since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    194328-plasmid-map-sequence-id-383152

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  20. pRS416-Gal4-dCas9-VP64

    Plasmid
    #71128
    Purpose
    This plasmid contains a Cas9 Activator for yeast. The activator is about 1.2-1.8 X more potent than just dCas9-VP64 fusion in yeast. It is on a single copy CEN/ARS plasmid with Ura marker, pRS416.
    Depositor
    Ronald Davis
    Publication
    Naranjo et al PLoS Genet. 2015 Dec 29;11(12):e1005751.
    Insert
    Gal4-dCas9-VP64
    Tags
    NLS n-terminal, N terminal Gal4 Activator domain,…
    Expression
    Yeast
    Promoter
    Tef1
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    71128-plasmid-map-sequence-id-127063

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Showing: 7581 - 7600 of 36352 results