We narrowed to 10,560 results for: fus
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Plasmid#130819PurposeExpresses mito-iFAST in mammalian cells (mitochondria labeling)DepositorInsertmito-iFAST
Tagsmyc-tagExpressionMammalianPromoterCMVAvailable SinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-SIN-BX SpyCatcher003-hCD52
Plasmid#210565PurposeExpresses Spycatcher003 on the cell surface using human CD52 hingeDepositorInsertSpycatcher003 fused to human CD52 hinge
UseLentiviralExpressionMammalianPromoterSFFVAvailable SinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-DIO ChRmine-oScarlet-KV 2.1-WPRE
Plasmid#183536PurposeOptogeneticsDepositorInsertChRmine-oScarlet-Kv2.1
UseAAVMutationNonePromoterCaMKIIaAvailable SinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF423
Plasmid#88083PurposeDonor Vector containing ZNF423 transcription factor, part of the Human TFome CollectionDepositorInsertZNF423 (ZNF423 Human)
UseGateway CloningMutationLast nucleotide of stop codon removed to allow fo…Available SinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJZC77
Plasmid#62338PurposesgRNA (no RNA aptamer addition) with COM-KRAB effector for mammalian cellsDepositorInsertssgRNA
COM-KRAB
UseLentiviralTagsKRABExpressionMammalianMutationTargets sv40 promoter, sequence: CATACTTCTGCCTGCT…PromoterCMV and U6Available SinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
MLM3727
Plasmid#49961PurposeExpresses a 3x Flag TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding siteDepositorAvailable SinceJan. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP-N1_CBPdCFIDKTG
Plasmid#221917PurposeTransient expression of GFP-tagged CBPdeltaCFIDKTGL in mammalian cellsDepositorInsertCBP (CREBBP Human)
TagsGFPExpressionMammalianMutationdeletion of the CBP-FUS interaction domain (CFID)…Available SinceJune 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
Mito-SNAP
Plasmid#206480PurposeEncodes a mitochondrially-targeted SNAP tag for mammalian cell expressionDepositorInsertMitochondrial targeting signal from COX8A fused to SNAP (derived from human MGMT)
TagsSNAPfExpressionMammalianPromoterCMVAvailable SinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-mNeonGreen-BRD4
Plasmid#204692PurposeThis plasmid allows for inducible expression of short BRD4 isoform tagged with mNeonGreen, in mammalian cells.DepositorAvailable SinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAH18
Plasmid#154397PurposeDerived from pGPI-SceI; Trimethoprim resistant; carries I-SceI gene; contains 475 bp from both upstream and downstream flanking regions between the FRT sites on pAHCTX1-rhadCas9DepositorInsertFused upstream and downstream homologous regions around FRT sites when pAH-CTX1-rhadCas9 integrated into K56-2 genome
UseSynthetic BiologyExpressionBacterialPromoterNAAvailable SinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-mAID-BE3
Plasmid#113420PurposeExpresses mAID-BE3 in mammalian cellsDepositorInsertmAID-BE3 (Aicda Mouse, S. pyogenes and Bacteriophage PBS2)
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAN19-3xFLAG-ccvTIR1-NOSt
Plasmid#108546PurposeCloning vector including N-terminally 3xFLAG-tagged Arabidopsis auxin receptor TIR1 with the F79G mutation [ccvTIR1] and NOS terminatorDepositorInsertTRANSPORT INHIBITOR RESPONSE 1 [F79G] fused with 3xFLAG and NOS terminator (TIR1 Mustard Weed)
UseCloning vectorTags3xFLAGMutationChanged F79 to GAvailable SinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRMC2-sec:msfGFP
Plasmid#194914PurposeTetracycline inducible expression of msfGFP fused to Sec signal peptide in StaphylococciDepositorInsertShine Dalgarno sequence followed by Sec signal peptide fused to monomeric superfolder GFP
UseTetracycline-inducible expression vector for stap…ExpressionBacterialAvailable SinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRMC2-tat:msfGFP
Plasmid#194915PurposeTetracycline inducible expression of msfGFP fused to Tat signal peptide in StaphylococciDepositorInsertShine Dalgarno sequence followed by Tat signal peptide fused to monomeric superfolder GFP
UseTetracycline-inducible expression vector for stap…ExpressionBacterialAvailable SinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGRE NPD-GFP
Plasmid#1197DepositorInsertN-terminus of Sup35 fused to GFP (SUP35 Budding Yeast)
TagsGFPExpressionYeastMutationfused N-terminal Sup35 (~1-253aa) to GFPAvailable SinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
mOC-STAMP_3xFlag-Myc-CMV26
Plasmid#73577PurposeExpress mouse OC-STAMP in mammalian cellsDepositorAvailable SinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
Cx43-sfGFP
Plasmid#70053PurposeExpresses rat Cx43 (Gja1 CDS) tagged with non-monomerized sfGFP on the C-terminus. CMV promoter.DepositorInsertCx43 (Gja1 Rat)
Tagsnon-monomerized superfolder GFP (V207)Mutationfused in frame to non-monomerized superfolder GFPPromoterCMVAvailable SinceOct. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL-SBP-EGFP-LAMP1
Plasmid#222317PurposeSynchronize the trafficking of LAMP1 from the ER.DepositorInsertStreptavidin-KDEL and LAMP1 fused to SBP-EGFP (LAMP1 Human)
ExpressionMammalianPromoterCMVAvailable SinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_CD63-SBP-mCherry
Plasmid#222326PurposeSynchronize the trafficking of CD63 from the ER.DepositorInsertStreptavidin-KDEL and CD63 fused to SBP-mCherry (CD63 Human)
ExpressionMammalianPromoterCMVAvailable SinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
EE-BE4max
Plasmid#138158PurposeC-to-T base editorDepositorInsertrAPOBEC1(EE)-nSpCas9-UGI-UGI
TagsNLSExpressionMammalianMutationWithin rAPOBEC1 R126E + R132E; within Cas9 D10APromoterCMVAvailable SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only