161,492 results

1. piggyBac-rtTA (4th_Gen)-sfGFP-IRES-NGN2-puro (VK_1122)

   Plasmid

   #209079

   Purpose

   Ngn2 mediated cortical neuron induction, along with sfGFP over-expression

   Depositor

   Vikram Khurana

   Article

   Lam et al bioRxiv 2022.11.08.515615

   Inserts

   sfGFP
   NGN2 (Neurog2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE4G

   Available since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-Mfn1

   Plasmid

   #141154

   Purpose

   Mammalian expression and labeling mitofusin 1

   Depositor

   Gia Voeltz

   Article

   Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   Mitofusin 1 (MFN1 Human)
   

   Use

   Tags

   AcGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-Nluc

   Plasmid

   #180501

   Purpose

   Mammalian expression of Nluc

   Depositor

   Masaharu Somiya

   Article

   Somiya et al bioRxiv 2022.01.25.477620

   Insert

   Nluc
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQE30-His-IF1

   Plasmid

   #124123

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Initiation factor 1 (infA Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. TRIPZ-bioGEF-UBwt-PURO

   Plasmid

   #208045

   Purpose

   Enables inducible expression of low-affinity Avitag-Ubwt; to perform bioE3; selection with puromycin

   Depositor

   James Sutherland, Rosa Barrio

   Article

   Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

   Insert

   Ub (UBC Human)
   

   Use

   Lentiviral

   Tags

   Low-affinity Avitag

   Expression

   Mutation

   Promoter

   tetO/UBC

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Drosophila_sgRNA_group_2

   Pooled Library

   #134583

   Purpose

   Targets Drosophila orthologs of vertebrate genes that have undergone expansion in the vertebrate line

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Elife. 2018 Jul 27;7. pii: 36333. doi: 1

   Expression

   Insect

   Species

   Drosophila melanogaster

   Use

   CRISPR

   Available since

   Feb. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

7. pUC-GFP-AT

   Plasmid

   #133306

   Purpose

   "Burdensome" GFP expressing plasmid carrying axe/txe toxin-antitoxin for plasmid stabilisation

   Depositor

   Chris Barnes

   Article

   Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

   Insert

   axe/txe toxin-antitoxin cassette
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF3083

   Plasmid

   #144559

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   FOSL2 (FOSL2 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLENTI-CMV-GFP-TREX1 D18N BLAST

   Plasmid

   #164225

   Purpose

   lentiviral plasmid for GFP-hTREX1 D18N (catalytically-deficient)

   Depositor

   John Maciejowski

   Article

   Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

   Insert

   TREX1 (TREX1 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   D18N

   Promoter

   CMV

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMXs-p53DD

    Plasmid

    #22729

    Depositor

    Shinya Yamanaka

    Article

    Hong et al Nature. 2009 Aug 27. 460(7259):1132-5.

    Insert

    Trp53 (Trp53 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    deleted amino acids 14-301

    Promoter

    Available since

    Dec. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDisplay-mSA-EGFP-TM

    Plasmid

    #39863

    Depositor

    Sheldon Park

    Article

    Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

    Insert

    monomeric streptavidin, EGFP, TM helix
    

    Use

    Tags

    EGFP, FLAG, and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFX-EGFP-Rab5

    Plasmid

    #174454

    Purpose

    Express EGFP with early endosome-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    EGFP with early endosome-targeting sequence
    

    Use

    Tags

    small GTPase (Rab5)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHW394 (15xUAS::GFP::let-858 3'UTR)

    Plasmid

    #85584

    Purpose

    cGAL effector (15xUAS::GFP::let-858 3'UTR) construct for C. elegans

    Depositor

    Paul Sternberg

    Article

    Wang et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

    Insert

    15xUAS-delta pes-10-GFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    GFP(S65C) with three introns for expression in C.…

    Promoter

    15xUAS-delta pes-10-GFP

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28 MBP_PrA/G-H6

    Plasmid

    #176077

    Purpose

    Express His6 tagged MBP_PrA/G in Escherichia coli

    Depositor

    Tom Rapoport

    Article

    Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

    Insert

    MBP PrA/G-H6
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCIS GEM-CEPIA1er

    Plasmid

    #58217

    Purpose

    Ratiometric (Blue to Green) fluorescent indicator for calcium signaling in the endoplasmic reticulum

    Depositor

    Masamitsu Iino

    Article

    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

    Insert

    GEM-CEPIA1er
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    GEM-GECO1 E298D F359W D400E E407D

    Promoter

    CAG

    Available since

    July 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. MaLionR

    Plasmid

    #113908

    Purpose

    Red ATP sensor

    Depositor

    Tetsuya Kitaguchi

    Article

    Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

    Insert

    MaLionR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenLox_U6 p53

    Plasmid

    #59360

    Purpose

    Lentiviral expression vector: p53 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.

    Depositor

    Peter Scheiffele

    Article

    Chih et al Science. 2005 Feb 25;307(5713):1324-8. E

    Inserts

    shRNA p53 (mouse) (Trp53 Mouse)
    eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and mouse U6

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBiFC-VN155(I152L)

    Plasmid

    #27097

    Depositor

    Chang-Deng Hu

    Article

    Kodama et al Biotechniques. 2010 Nov . 49(5):793-805.

    Insert

    VN155(I152L)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    I152L

    Promoter

    Available since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pQCXIN-TetR-mCherry

    Plasmid

    #59417

    Purpose

    retroviral construct expressing the Tet repressor (TetR) tagged with mCherry

    Depositor

    Tom Misteli

    Article

    Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

    Insert

    Tet repressor protein (TetR)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPROEX Aqua

    Plasmid

    #42889

    Depositor

    Fabienne Merola

    Article

    Erard et al Mol Biosyst. 2013 Feb 2;9(2):258-67. doi

    Insert

    Aquamarine
    

    Use

    Tags

    6xHis and TEV cleavage

    Expression

    Bacterial

    Mutation

    T65S and H148G

    Promoter

    Available since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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