We narrowed to 9,675 results for: BLI

1. Galt1-sCGrx1p-HA

   Plasmid

   #230028

   Purpose

   Expressing sCGrx1p protein fused to HA tag in mammalian cells with localization in the Golgi apparatus.

   Depositor

   Pablo Rivera-Fuentes

   Insert

   Galt1-sCGrx1p-HA (B4GALT1 Synthetic, Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. B52_puro_empty_gRNA

   Plasmid

   #197557

   Purpose

   sites for cloning two gRNA with puromycin cassette

   Depositor

   Darius Bägli

   Type

   Empty backbone

   Use

   Empty grna backbone with puromycin resistance gene

   Expression

   Mammalian

   Promoter

   U6 for gRNA, CMV for puromycin resistance.

   Available Since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. RabV CVS-N2c(deltaG)-dsRed

   Plasmid

   #73460

   Purpose

   Expresses dsRed for monosynaptic circuit mapping

   Depositor

   Thomas Jessell

   Article

   Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

   Insert

   dsRed
   

   Use

   Neurotropic virus

   Available Since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. RabV CVS-N2c(deltaG)-Cre

   Plasmid

   #73474

   Purpose

   Expresses Cre for circuit mapping and genetic manipulation

   Depositor

   Thomas Jessell

   Article

   Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

   Insert

   Cre recombinase
   

   Use

   Neurotropic virus

   Available Since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pL1P5 ZmUbiP:GRF-GIF:NosT

   Plasmid

   #198046

   Purpose

   MoClo Golden Gate Level 1 Position 5. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.

   Depositor

   Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing

   Insert

   ZmUbiP::TaGRF4-GIF1::NosT
   

   Use

   Synthetic Biology

   Promoter

   Zea mays (maize) ubiquitin promoter

   Available Since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-T7-SpRY-P2A-mScarlet (MNW002)

   Plasmid

   #174137

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R); C-terminal bi-partite NLS, 3x flag tag, P2A-mScarlet

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized SpRY with BPNLS-3xFLAG-P2A-mScarlet
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-mScarlet

   Expression

   Mammalian

   Mutation

   SpG=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317…

   Promoter

   CMV and T7

   Available Since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti HsATP10B_D433N-T2A-His-flag-TMEM30A

   Plasmid

   #191676

   Purpose

   transfer plasmid for lentiviral vector production expressing Hs ATP10B D433N mutant and His/flag tagged Hs TMEM30A

   Depositor

   Veerle Baekelandt

   Inserts

   ATP10B (ATP10B Human)
   TMEM30A (TMEM30A Human)
   

   Use

   Lentiviral

   Tags

   6x HIS and flag

   Mutation

   D433N

   Available Since

   Nov. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPyCAG-tagBFP-3xNLS-IRES-Hph

   Plasmid

   #183608

   Purpose

   Mammalian expression vector for tagBFP-3xNLS from the CAG promoter. Confers hygromycin B resistance.

   Depositor

   Sally Lowell

   Article

   Malaguti et al Development. 2022 May 26. pii: 275525. d

   Insert

   tagBFP-3xNLS
   

   Tags

   3xNLS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-SFFV-ZFYVE27-WPRE-UbC-mCherry

   Plasmid

   #214470

   Purpose

   Lentiviral vector plasmid expressing human zinc finger FYVE-type containing 27 (ZFYVE27) under the spleen focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin C (UbC) promoter

   Depositor

   Richard Eva, Bart Nieuwenhuis

   Inserts

   ZFYVE27 (ZFYVE27 Human)
   mCherry
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCerulean-hCOL1A1

    Plasmid

    #140112

    Purpose

    Expresses fluorescently-tagged human Type I procollagen α1 chain with mCerulean replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

    Depositor

    Sergey Leikin

    Insert

    Type I procollagen α1 (COL1A1 Human)
    

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    COL1A1 exons 2-3 replaced by fluorescent tag

    Promoter

    CMV

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFP.R265

    Plasmid

    #138167

    Purpose

    Expresses the split mCherry reporter interrupted by the SspDnaB M86 artificially mini-intein (in cis)

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    mCherryN(VDA).M86.(SDL)mCherryC.H6
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    P(araBAD) and iPC

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQTEV-PPP1R1A

    Plasmid

    #31331

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    protein phosphatase 1 (PPP1R1AP1 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQTEV-CIP29

    Plasmid

    #34822

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    cytokine induced protein 29 kDa (SARNP Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDFDuet-trAtCCD7-OsD27(pYL726 )

    Plasmid

    #178285

    Purpose

    Co-Expresses OsD27 and trAtCCD7 in E. coli. pCDFDuet-1 carrying D27 from Oryza sativa and N-terminus 31 amino-acid truncated CCD7 from Arabidopsis

    Depositor

    Yanran Li

    Article

    Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

    Inserts

    OsD27
    trAtCCD7
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. FLAG-hLRP6-opt(20-1613)-9xHis_MESD_pFastBacDual

    Plasmid

    #216377

    Purpose

    Baculovirus transfer vector to co-express FLAG-LRP6-His (human sequence, codon-optimized for insect cell expression) and MESD chaperone (human sequence)

    Depositor

    William Weis

    Insert

    LDL receptor related protein 6 (LRP6 Human)
    

    Use

    Baculovirus

    Tags

    9xHis and HA signal sequence-FLAG-3C cleavage sit…

    Expression

    Insect

    Promoter

    Polyhedrin (LRP6)/p10 (MESD)

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. dABE

    Plasmid

    #124447

    Purpose

    Expresses dead Cas9 (dCas9)-ABE7.10

    Depositor

    George Church

    Article

    Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

    Insert

    dABE7.10
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Available Since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-UbC-mCherry Rab5

    Plasmid

    #115239

    Purpose

    To express in mammalian cells the endocytic protein Rab5 fused to mCherry by transfection or by generating AAV virions.

    Depositor

    Pantelis Tsoulfas

    Insert

    Rab5a (RAB5A Homo sapiens, Human)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    Human UbC

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. lentiMutB3GNT5-blast

    Plasmid

    #208380

    Purpose

    lentiviral vector for expressing human B3GNT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

    Depositor

    Jennifer Kohler

    Insert

    Beta-1,3-N-Acetylglucosaminyltransferase 5 (B3GNT5 Human)
    

    Use

    Lentiviral

    Tags

    myc, FLAG

    Expression

    Mammalian

    Mutation

    nt 922 c->g (PAM site inactivation)

    Promoter

    EF-1 alpha core promoter

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pQTEV-PDAP1

    Plasmid

    #31586

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    PDGFA associated protein 1 (PDAP1 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pQTEV-SNX3

    Plasmid

    #31573

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    sorting nexin 3 (SNX3 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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