We narrowed to 20,306 results for: IRE;
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G-CaMP3
Plasmid#22692Purposea single-wavelength GCaMP2-based genetically encoded calcium indicatorDepositorInsertG-CaMP3
ExpressionMammalianMutationN363D mutation (increased fluorescence)Available SinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pmGFP-P2A-K0-P2A-RFP
Plasmid#105686PurposeDouble fluorescent stalling reporter K0DepositorInsertsP2A-3xFLAG-VHPbeta
K0
P2A-mCherry
ExpressionMammalianPromoterCMV (from vector)Available SinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmGFP-P2A-K(AAA)20-P2A-RFP
Plasmid#105688PurposeDouble fluorescent stalling reporter K(AAA)20DepositorInsertsP2A-3xFLAG-VHPbeta
K(AAA)20
P2A-mCherry
ExpressionMammalianPromoterCMV (from vector)Available SinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
NylCK-TS_pET28a
Plasmid#215423PurposeExpression construct for NylCK-TS gene, codon optimized for expression in E. coliDepositorInsertNylCK-TS
ExpressionBacterialMutationS111G/A137LAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
lenti-tmpknot-epegRNA-puro backbone
Plasmid#214089PurposeLentiviral backbone plasmid to insert epegRNAs (mpknot) of interest. The cloning site is BsmbI.DepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterhU6, EF1aAvailable SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSynapsin_psychLight2
Plasmid#163909PurposeTo generate Adeno-Associated viruses for expression of psychLight2 under synapsin promoterDepositorHas ServiceAAV9InsertpsychLight2
UseAAVTagsFlag tagExpressionMammalianPromoterSynapsinAvailable SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.iGABASnFR
Plasmid#112172PurposeGABA SensorDepositorInsertiGABASnFR
UseAAVPromoterGFAPAvailable SinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
FUGW-d2GFP-ZEB1 3'UTR
Plasmid#79601PurposeFUGW lentiviral vector with constitutively expressed destabilized GFP (d2GFP) regulated by the human ZEB1 3'UTR. When used in combination with Ecad-RFP, comprises the Z-cad dual sensor.DepositorInsertd2GFP-Zeb1 3'UTR
UseLentiviralAvailable SinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV hU6-gRNA(anti-sense) hUbC-VP64-dCas9-VP64-T2A-GFP
Plasmid#66707Purposeexpresses a single gRNA and VP64-dCas9-VP64DepositorInsertshU6-gRNA expression cassette
S.Pyogenes VP64-dCas9-VP64
UseLentiviral and Synthetic BiologyTagsVP64MutationD10A and H840APromoterhU6 and hUbCAvailable SinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
MBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5
Plasmid#135454PurposeBacterial expression of TEVΔ proteaseDepositorInsertMBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5
TagsMBP, His6ExpressionBacterialMutationS219V mutation improves stability. Truncated form…Promotertac promoterAvailable SinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
NLRP3-mNeonGreen
Plasmid#200942PurposeNLRP3 fused with mNeonGreen to indicate its localization changeDepositorInsertNLRP3 (NLRP3 Human)
UseLentiviralAvailable SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsLSm1_J
Plasmid#146676PurposeMammalian Expression of HsLSm1DepositorInsertHsLSm1 (LSM1 Human)
ExpressionMammalianAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
WT TXNIP-FLAG
Plasmid#200091PurposeWT human TXNIP with C-terminal 3xFLAG tag in pQCXIP vectorDepositorAvailable SinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
myc-TRX
Plasmid#21614DepositorAvailable SinceNov. 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
7a sgRNA for EJ7-GFP and 4-μHOM reporters
Plasmid#113620PurposesgRNA/CAS9 expression plasmid to induce the 5’ double-strand break in both the EJ7-GFP and 4-μHOM reportersDepositorInsert7a sgRNA
UseCRISPRExpressionMammalianAvailable SinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
7b sgRNA for EJ7-GFP reporter
Plasmid#113624PurposesgRNA/CAS9 expression plasmid to induce the 3’ double-strand break in the EJ7-GFP reporterDepositorInsert7b sgRNA
UseCRISPRExpressionMammalianAvailable SinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD24-sfGFPx2
Plasmid#51559PurposeSuperfolder GFP ORF cloned into pBAD24 for expression in E. coli. It contains additional aminoacids in the N and C terminus (polilinker sequences for cloning proteins in frame with GFP)DepositorInsertsuperfolder GFP
TagsGLESTCRHASLAVLADERRFSA and MARARAExpressionBacterialMutationContains additonal aa in the N and C terminus (in…Available SinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
p11-LacY-wtx1
Plasmid#69056PurposeThe reporter plasmid p11-LacY-wtx1 encodes the toxin CcdB gene under the inducible BAD promoter and a single copy of the endonuclease cleavage site which is readily extendable to multiple copies.DepositorAvailable SinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGCDNsam-Hnf4a
Plasmid#33002DepositorInserthepatic nuclear factor 4 alpha (Hnf4a Mouse)
UseRetroviralAvailable SinceFeb. 27, 2012AvailabilityAcademic Institutions and Nonprofits only