We narrowed to 9,364 results for: tre promoter

1. hAID-BE3 (pRZ352)

   Plasmid

   #131316

   Purpose

   CAG promoter expression plasmid for hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

   Insert

   hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO.1-shGBP1.1.mKO2

   Plasmid

   #85209

   Purpose

   TRCN0000116119 (Target CGACGAAAGGCATGTACCATA) for silencing GBP1 gene and express monomeric Kusabira-Orange2.

   Depositor

   Sandra Martha Gomes Dias

   Article

   Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

   Insert

   GBP1 (guanylate binding protein 1) (GBP1 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   RNA polymerase III promoter for human U6 snRNA fo…

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAc5-STABLE2-neo-RvCAHS3

   Plasmid

   #192472

   Purpose

   Stable expression of RvCAHS3 (fly codon optimized) in Drosophila cells

   Depositor

   Takekazu Kunieda

   Article

   Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

   Insert

   CAHS3
   

   Tags

   T2A-EGFP-T2A-neoR

   Expression

   Insect

   Promoter

   Ac5 promoter

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO.1-shGBP1.2.mKO2

   Plasmid

   #85210

   Purpose

   TRCN0000116120 (Target TGAGACGACGAAAGGCATGTA) for silencing GBP1 gene and express monomeric Kusabira-Orange2.

   Depositor

   Sandra Martha Gomes Dias

   Article

   Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

   Insert

   GBP1 (guanylate binding protein 1) (GBP1 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   RNA polymerase III promoter for human U6 snRNA fo…

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEN_TmiR_G12-E

   Plasmid

   #25761

   Purpose

   Entry vector with TRE promoter driving mouse G alpha 12 miR30-based shRNA.

   Depositor

   Iain Fraser

   Article

   Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

   Insert

   G alpha 12 miR-shRNA (Gna12 Mouse)
   

   Use

   Entry vector

   Available Since

   July 14, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPtGE34

   Plasmid

   #107932

   Purpose

   Expresses Cas9 in Phaeodactylum tricornutum / Encodes elements required for conjugation

   Depositor

   David Edgell

   Article

   Slattery et al ACS Synth Biol. 2018 Jan 8. doi: 10.1021

   Inserts

   OriT
   40SRPS8 Promoter
   ShBle
   40SRPS8 Terminator
   Cen6-ArsH4-His3
   

   Use

   CRISPR and Synthetic Biology; Episomal vector for…

   Available Since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKE12-ISceI-fabp10a:loxP-BFP-loxP-Xla.Ctnnb1

   Plasmid

   #198240

   Purpose

   For I-SceI-mediated transgenesis in zebrafish; fabp10a promoter drives expression of activated β-catenin preceded by a blue fluorescent protein (BFP)-STOP cassette flanked by loxP sites

   Depositor

   Didier Stainier

   Article

   Kalasekar et al Biol Open. 2019 Oct 17;8(10):bio047829.

   Inserts

   Zebrafish fabp10a promoter:floxedBFP, Xenopus pt-beta-catenin coding sequence, polyA (fabp10a Frog)
   Zebrafish cryaa promoter:mcherry fluorescent protein coding sequence (cryaa Zebrafish)
   

   Use

   Zebrafish transgenesis

   Available Since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. BE3-P2A-EGFP (pJUL977)

   Plasmid

   #123612

   Purpose

   CAG promoter expression plasmid for rAPOBEC1-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP.

   Depositor

   Keith Joung

   Article

   Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

   Insert

   BE3-P2A-EGFP
   

   Expression

   Mammalian

   Mutation

   D10A in SpCas9

   Promoter

   CAG

   Available Since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. eA3A-BE3 (pRZ206)

   Plasmid

   #131315

   Purpose

   CAG promoter expression plasmid for hA3A(N57G)-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

   Insert

   hA3A(N57G)-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. NLS-nCas9-NLS-P2A-EGFP (pRZ70)

    Plasmid

    #123616

    Purpose

    CMV promoter expression plasmid for codon-optimized bpNLS-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP (ABEmax control without TadA domains).

    Depositor

    Keith Joung

    Article

    Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

    Insert

    NLS-nCas9-NLS-P2A-EGFP
    

    Expression

    Mammalian

    Mutation

    D10A in SpCas9

    Promoter

    CMV

    Available Since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGB2Ω2_SF-35s:hCas9:tNos (GB1103)

    Plasmid

    #75400

    Purpose

    Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter. Specially conceived to be linked with the TU of the kanamycin resistance gene GB1181.

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

    Insert

    hCas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Promoter

    35S

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pORANGE_Tubb3 GFP(Y39TAG) KI

    Plasmid

    #182678

    Purpose

    Expression of spCas9, gRNA targeting the end of Tubb3 gene and donor GFP(Y39TAG). Can be used for amber codon suppression and click chemistry labeling of endogenous beta-3 tubulin in mammalian cells.

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Insert

    Tubb3-targeting gRNA and GFP(Y39TAG) donor sequence (Tubb3, Tubb3 Rat, Mouse)
    

    Expression

    Mammalian

    Promoter

    U6 and chicken beta-actin promoter

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. N295A (EcN), E166Q (EcN) MetNI LmH

    Plasmid

    #118269

    Purpose

    N295A and E166Q Mutation of MetN in Methionine ABC Transporter with 6x His Tag. Used to solve crystal structure

    Depositor

    Douglas Rees

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

    Inserts

    MetI (metI Escherichia coli)
    MetN (E166Q ; N295A) (metN Escherichia coli)
    

    Tags

    6x His Tag

    Expression

    Bacterial

    Mutation

    Changed Glutamic Acid (Glu) 166 to Glutamine (Gln…

    Promoter

    T7 Promoter

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEMS1494

    Plasmid

    #29242

    Purpose

    Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).

    Depositor

    Elizabeth Simpson

    Article

    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

    Insert

    Ple23 (CCKBR Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Intron-LacZ-NLS

    Available Since

    Nov. 7, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pEMS1493

    Plasmid

    #29241

    Purpose

    Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).

    Depositor

    Elizabeth Simpson

    Article

    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

    Insert

    Ple22 (CCKBR Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Intron-LacZ-NLS

    Available Since

    Nov. 7, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pfast bac cry1myc

    Plasmid

    #51892

    Purpose

    Backbone plasmid is pfastBacHTa from invitrogen

    Depositor

    Joseph Takahashi

    Article

    Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

    Insert

    cry1 (Cry1 Mouse)
    

    Tags

    3x Flag and myc

    Promoter

    polyhedrin promoter

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pCW57-Cx46-IRES-GCaMP6s

    Plasmid

    #188238

    Purpose

    3rd generation, inducible bicistronic lentiviral plasmid for expression of human connexin 46 and GCaMP6s

    Depositor

    Fabio Mammano

    Article

    Nardin et al Int J Mol Sci. 2022 Jun 30;23(13). pii:

    Insert

    GJA3 (GJA3 Human)
    

    Use

    Lentiviral

    Tags

    GCaMp6s

    Expression

    Mammalian

    Promoter

    Tight TRE promoter

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGLS3-5xHRE-p53(K164R)

    Plasmid

    #72555

    Purpose

    Hypoxia induced mutant p53. Contains the HRE from VEGF sequence (5 repeats) upstream of p53 K164R.

    Depositor

    Ester Hammond

    Article

    Leszczynska et al J Clin Invest. 2015 Jun;125(6):2385-98.

    Insert

    p53 (TP53 Human)
    

    Tags

    5XHRE

    Expression

    Mammalian

    Mutation

    K164R

    Promoter

    E1B minimal promoter

    Available Since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. tetO-AKT1

    Plasmid

    #170682

    Purpose

    doxycycline-inducible overexpression of AKT1

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet-ON

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCW57.1-Eomes-3xT7

    Plasmid

    #200888

    Purpose

    Inducible lentiviral expression of Eomes

    Depositor

    Kai Ge

    Article

    Xie et al Nat Genet. 2023 Apr 3. doi: 10.1038/s415

    Insert

    Eomes (Eomes Mouse)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    3xT7

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet ON

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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