We narrowed to 23,367 results for: Nov;

1. H6GB1tevP53(1-303)IntN

   Plasmid

   #200312

   Purpose

   Bacterial expression of P53 NTAD-DBD for segmental labeling

   Depositor

   Peter Wright

   Article

   Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   His6GB1tev and designed intein N

   Expression

   Bacterial

   Mutation

   M133L, V203A, N239Y, N268D

   Promoter

   T7

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-WT-VLCAD-FLAG

   Plasmid

   #55823

   Purpose

   Expresses WT-VLCAD in mammalian cells

   Depositor

   Yoshimi Homma

   Article

   Kabuyama et al Am J Physiol Cell Physiol. 2010 Jan;298(

   Insert

   Very long-chain specific acyl-CoA dehydrogenase (ACADVL Human)
   

   Use

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBMN Parkin

   Plasmid

   #89299

   Purpose

   For expression of untagged Parkin

   Depositor

   Michael Lazarou

   Article

   Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

   Insert

   Parkin (PRKN Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. RRL.sin.cPPT.SFFV/Flag-E2-crimson.IRES-puro.WPRE (CG568)

   Plasmid

   #139445

   Purpose

   Lentiviral vector to ectopically express Flag-tagged E2-crimson from SFFV promoter

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   FLAG-tag E2-crimson
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVXT-mWnt3

   Plasmid

   #121934

   Purpose

   doxycycline-inducible mouse Wnt3

   Depositor

   Joan Massague

   Article

   Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

   Insert

   Wnt3 (Wnt3 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. LentiGuide-Puro-GFPg1 (BB09)

   Plasmid

   #139461

   Purpose

   Lentiviral vector with gRNA targeting GFP; includes puromycin selectable marker

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   GFP-targeting sgRNA inserted; resistance gene: puroR
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a / U6

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLHCX-Flag-mPKM2(C358S)

   Plasmid

   #42513

   Depositor

   Dimitrios Anastasiou

   Article

   Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

   Insert

   Pyruvate Kinase M2 Cys358->Ser
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mutation

   Changed Cysteine 358 to Serine

   Promoter

   CMV

   Available since

   March 19, 2013

   Availability

   Academic Institutions and Nonprofits only

8. Agarabi CHO Heavy Chain in pJ201

   Plasmid

   #111567

   Purpose

   Cloning vector containing CDS for the modified human heavy chain of the model chimeric mouse-human IgG1 expressed by the Agarabi CHO cell line

   Depositor

   Cyrus Agarabi

   Article

   Velugula-Yellela et al Biotechnol Prog. 2018 Jan;34(1):262-270.

   Insert

   Human heavy chain
   

   Use

   Synthetic Biology; Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET21-pelB-LaG-11

   Plasmid

   #172762

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-11; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-11
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET21-pelB-LaM-1

    Plasmid

    #172767

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-1, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-1
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Agarabi CHO Light Chain in pJ201

    Plasmid

    #111566

    Purpose

    Cloning vector containing CDS for the murine light chain of the model chimeric mouse-human IgG1 expressed by the Agarabi CHO cell line

    Depositor

    Cyrus Agarabi

    Article

    Velugula-Yellela et al Biotechnol Prog. 2018 Jan;34(1):262-270.

    Insert

    Murine light chain
    

    Use

    Synthetic Biology; Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCDNA3.1Xpress-AbrGAP

    Plasmid

    #38177

    Depositor

    Nora Heisterkamp

    Article

    Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

    Insert

    Abr GAP domain (ABR Human)
    

    Use

    Tags

    Xpress

    Expression

    Mammalian

    Mutation

    ABR GAP domain including amino acids 597-859

    Promoter

    Available since

    Sept. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

13. LentiGuide-Neo-CTRLg2

    Plasmid

    #139451

    Purpose

    Lentiviral vector with non-targeting gRNA and neomycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    Non-targeting sgRNA inserted; resistance gene: neoR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a / U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET21-pelB-LaG-29

    Plasmid

    #172753

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-29; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-29
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LentiGuide-Puro-CTRLg1 (CG509)

    Plasmid

    #139455

    Purpose

    Lentiviral vector with non-targeting gRNA and puromycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    Non-targeting sgRNA inserted; resistance gene: puroR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a / U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Flag-MCPIP1(1-555)

    Plasmid

    #90105

    Purpose

    Mammalian expression of Flag-MCPIP1(1-555)

    Depositor

    Mingui Fu

    Article

    Liang et al J Exp Med. 2010 Dec 20;207(13):2959-73.

    Insert

    MCPIP1 (ZC3H12A Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCIBN-tagBFP-hPMLIII

    Plasmid

    #103807

    Purpose

    BLInCR 'Localizer' construct that marks the PML nuclear bodies and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-tagBFP-hPMLIII (CIB1, PML Human, Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCellulose

    Plasmid

    #36300

    Depositor

    Jay Keasling

    Article

    Bokinsky et al Proc Natl Acad Sci U S A. 2011 Dec 13;10

    Insert

    pCellulose
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Two promoters from the E coli genome drive two di…

    Available since

    Nov. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a-His-hPKM2

    Plasmid

    #42515

    Depositor

    Dimitrios Anastasiou

    Article

    Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

    Insert

    Pyruvate Kinase M2 (PKM Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

20. ECFP-Bcr in pCCL-cppt178-MNDU3

    Plasmid

    #38156

    Depositor

    Nora Heisterkamp

    Article

    Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

    Insert

    Bcr (BCR Human)
    

    Use

    Lentiviral

    Tags

    ECFP

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

21. LentiGuide-Neo-CTRLg1

    Plasmid

    #139450

    Purpose

    Lentiviral vector with non-targeting gRNA and neomycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    Non-targeting sgRNA inserted; resistance gene: neoR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a / U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. MERS-coV-NSP3-c001

    Plasmid

    #173085

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    rep (orf1ab Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) (MERS-CoV))
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    D1109-D1275, codon optimized

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET21-pelB-LaG-17

    Plasmid

    #172747

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-17; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-17
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-CAG-soCoChR-GFP

    Plasmid

    #107710

    Purpose

    A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. CAG promoter

    Depositor

    Edward Boyden

    Article

    Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

    Insert

    soCoChR-GFP
    

    Use

    AAV

    Tags

    EGFP and KA2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBMN HA-GBRP

    Plasmid

    #89296

    Purpose

    For expression of the mammalian Atg8 protein GABARAP tagged with HA

    Depositor

    Michael Lazarou

    Article

    Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

    Insert

    GABARAP (GABARAP Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

26. pSIL-eGFP-shACTN3

    Plasmid

    #52678

    Purpose

    shRNA against α-actinin-3 with eGFP transfection marker

    Depositor

    Johannes Hell

    Article

    Schnizler et al J Biol Chem. 2009 Jan 30;284(5):2697-705

    Insert

    ACTN3 (Actn3 Rat)
    

    Use

    RNAi

    Tags

    CMV-EGFP

    Expression

    Mutation

    Promoter

    U6

    Available since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pPHR-mCherry-hPMLIII

    Plasmid

    #103824

    Purpose

    Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    PHR-mCherry-hPMLIII (CRY2, PML Human, Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV-NLS-FRB-VP16

    Plasmid

    #210515

    Purpose

    expresses NLS-FRB-VP16 component in mammalian cells

    Depositor

    Dongmin Lee

    Article

    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

    Insert

    NLS-FRB-VP16
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMX-IY LC3C

    Plasmid

    #89292

    Purpose

    For expression of the mammalian Atg8 protein LC3C without a tag

    Depositor

    Michael Lazarou

    Article

    Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

    Insert

    LC3C (MAP1LC3C Human)
    

    Use

    Retroviral

    Tags

    IRES-YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLHCX-Flag-mPKM2(K433E)

    Plasmid

    #42514

    Depositor

    Dimitrios Anastasiou

    Article

    Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

    Insert

    Pyruvate Kinase M2 Lys433->Glu
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mutation

    Changed lysine 433 to glutamate

    Promoter

    Available since

    March 19, 2013

    Availability

    Academic Institutions and Nonprofits only