We narrowed to 23,357 results for: BASE

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ClhN-pADH3-4V5-APEX2-URA

Plasmid

#207063

Purpose

Backbone plasmid containing ADH3 promoter and 4V5-APEX2 tag.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

4xV5-APEX2

Expression

Yeast

Promoter

pADH3

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-cTP-TurboID

Plasmid

#209394

Purpose

Transiently expressing TurboID fused with a chloroplast transit peptide (cTP) in planta

Depositor

Rosa Lozano-Durán

Article

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Insert

cTP-3XHA-TurboID

Expression

Plant

Promoter

Cauliflower mosaic virus (CaMV) 35S promoter

Available Since

Jan. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pEF1α-Cre-2A-miRFP670nano3

Plasmid

#207747

Purpose

Expression of the Cre recombinase linked to a miRFP670nano3 fluorescent marker

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

Cre-PGK-miRFP670nano3

Use

Cre/Lox

Expression

Mammalian

Promoter

EF1alpha

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pABY-T3

Plasmid

#201766

Purpose

For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast

Depositor

Saravanan Palani

Article

Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

Insert

ALFA tag

Expression

Yeast

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pICH-roGFP2-GPXC71S

Plasmid

#191698

Purpose

Ti plasmid pICH86988 harbouring roGFP2-GPXC71S -a control roGFP2 fusion not responsive to peroxides used alongside plasmids 191680 and 191677

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

Insert

redox(ro)GFP2-GPXC71S

Expression

Plant

Mutation

Peroxidatic Cysteine 71 replaced with Serine

Promoter

CaMV 35S

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-IL2sp-6His-Asprosin plasmid

Plasmid

#190683

Purpose

Expresses His-tagged asprosin in mammalian cells. An IL2 signal peptide at N-terminus allows secretion of asprosin from the transduced cells.

Depositor

Atul Chopra

Article

Mishra et al STAR Protoc. 2022 Dec 16;3(4):101762. do

Insert

IL2-6xHis-Asprosin

Tags

IL2 signal peptide, 6xHis

Expression

Mammalian

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pIAA5::LUC

Plasmid

#141169

Purpose

Luminescent reporter for auxin signaling

Depositor

Vardis Ntoukakis, Patrick Schäfer

Article

Lehmann et al PLoS One. 2020 Jun 4;15(6):e0234154. doi

Insert

AtIAA5 promoter

Use

Luciferase

Expression

Plant

Promoter

AtIAA5, At1g15580

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pRAB18::LUC

Plasmid

#141168

Purpose

Luminescent reporter for abscisic acid signaling

Depositor

Vardis Ntoukakis, Patrick Schäfer

Article

Lehmann et al PLoS One. 2020 Jun 4;15(6):e0234154. doi

Insert

AtRAB18 promoter

Use

Luciferase

Expression

Plant

Promoter

AtRAB18, AT5G66400

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pJT43_GalL_BE-P(AP)3

Plasmid

#145036

Purpose

Expresses BE-P(AP)3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

BE-(AP)3

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A)

Promoter

GalL

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pJL009

Plasmid

#122862

Purpose

Binary expression vector containing 35S:MS2-SRDX:tRBCS followed by GreenGate A-G sequences for insertion of additional modules

Depositor

Markus Schmid

Article

Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

Insert

35S:MS2-SRDX:tRBCS

Use

Golden gate compatible plant transformation vector

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pSB1A3-AD009

Plasmid

#116850

Purpose

Expresses araC constitutively and alpha-hemolysin under a pBAD promoter; alternate name pSB1A2-AD009

Depositor

Friedrich Simmel

Article

Dupin et al Nat Chem. 2018 Nov 26. pii: 10.1038/s415

Inserts

AraC
alpha-HL

Use

Synthetic Biology

Expression

Bacterial

Promoter

pBAD

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pJC-20xUAS-TALE3-VP64-P10

Plasmid

#104607

Purpose

Expresses TALE3 under UAS control

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

TALE3

Use

TALEN

Expression

Insect

Promoter

hsp70

Available Since

Jan. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCXCR4_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

Plasmid

#101113

Purpose

Encodes dCas9(C)-VP64 with SAM components fused to CXCR4

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

CXCR4-dCas9(C)-VP64

Use

CRISPR and Synthetic Biology

Tags

Flag and HA

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/WT SPAST without 5'UTR

Plasmid

#87732

Purpose

Expresses WT spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Expression

Mammalian

Mutation

5'UTR deleted (1-190 bp)

Promoter

CMV

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-SBP

Plasmid

#67173

Purpose

CRISPaint universal donor for streptavidin binding peptide fusion tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

SBP

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-1xSunTag

Plasmid

#67177

Purpose

CRISPaint universal donor for SunTag fusion tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

1xSunTag v4

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pKS043

Plasmid

#65471

Purpose

pTrc plasmid expressing clpS

Depositor

Keith Tyo

Article

Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

Insert

clpS (clpS )

Use

Synthetic Biology

Expression

Bacterial

Promoter

trc

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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8X2C mAGNET

Plasmid

#67955

Purpose

Lentiviral vector coding EGFP followed by 8x miR-128 and 8x miR-221 target sequences

Depositor

Xue Han, Wilson Wong

Article

Sayeg et al ACS Synth Biol. 2015 Apr 13.

Type

Empty backbone

Use

Lentiviral

Available Since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pTHSSd_42

Plasmid

#59965

Purpose

beta core fragment expression

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

beta core fragment

Expression

Bacterial

Mutation

N/A

Promoter

PJ23105

Available Since

Dec. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pTHSSd_10

Plasmid

#59950

Purpose

PK1F GFP reporter

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

sfGFP

Expression

Bacterial

Mutation

N/A

Promoter

PK1F

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only

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