Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 8,587 results for: construct

Clear keywords
Showing: 7701 - 7720 of 8587 results

  1. FUW-crRNA-1-U6_TRE-dLbCpf1-NLS-hCTCF_WT-HA-P2A-tagBFP

    Plasmid
    #194889
    Purpose
    Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-WT and crRNA-1 targeting the human MECP2 locus.
    Depositor
    Shawn Liu
    Article
    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd
    Inserts
    dLbCpf1-NLS-hCTCF_WT
    U6-crRNA1
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Human codon optimized catalytically inactive LbCp…
    Promoter
    TRE and U6
    Available Since
    Jan. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    194889-plasmid-map-sequence-id-382866

    Enlarge

  2. FUW-crRNA-2-U6_TRE-dLbCpf1-NLS-hCTCF_WT-HA-P2A-tagBFP

    Plasmid
    #194893
    Purpose
    Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-WT and crRNA-2 targeting the human MECP2 locus.
    Depositor
    Shawn Liu
    Article
    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd
    Inserts
    dLbCpf1-NLS-hCTCF_WT
    U6-crRNA2
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Human codon optimized catalytically inactive LbCp…
    Promoter
    TRE and U6
    Available Since
    Jan. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    194893-plasmid-map-sequence-id-382802

    Enlarge

  3. pSN-A112C-1A31

    Plasmid
    #191242
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A31 and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Article
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available Since
    Jan. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    191242-plasmid-map-sequence-id-382360

    Enlarge

  4. pSN-A112C-CP8B1

    Plasmid
    #191240
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-CP8B1 and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Article
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), CYP8B1 (CYP12) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    191240-plasmid-map-sequence-id-380472

    Enlarge

  14. pMH011_AAVS1_puro-pA-9xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry

    Plasmid
    #179434
    Purpose
    Dual fluorescent reporter construct with single HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus
    Depositor
    Lacra Bintu
    Article
    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1
    Insert
    TRE-cit-HS4-mch (SH)
    Expression
    Mammalian
    Promoter
    pEF alpha, pRSV
    Available Since
    March 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    179434-plasmid-map-sequence-id-355565

    Enlarge

  17. pDestTol2-actinb-Hs.KCNJ13-IRES-EGFP

    Plasmid
    #164945
    Purpose
    Tol2 construct: actinb (actb2) promoter driven human KCNJ13 and EGFP”. This will reflect the linkage of KCNJ13 and EGFP
    Depositor
    GuangJun Zhang
    Article
    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi
    Insert
    KCNJ13 (KCNJ13 Human)
    Use
    Tol2 transposon destination vector
    Tags
    IRES-EGFP
    Mutation
    Stop codon deleted
    Promoter
    actinb (actb2) promoter
    Available Since
    March 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    164945-plasmid-map-sequence-id-325850

    Enlarge

  18. pDestTol2-actinb-kcnj13-Q153H-EGFP

    Plasmid
    #164943
    Purpose
    Tol2 construct: actinb (actb2) promoter driven zebrafish loss-of-functionkcnj13 mutant, Q153H and M154I
    Depositor
    GuangJun Zhang
    Article
    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi
    Insert
    kcnj13 (kcnj13 Zebrafish)
    Use
    Tol2 transposon destination vector
    Tags
    EGFP
    Mutation
    Stop codon deleted; Q153H; M154I
    Promoter
    actinb (actb2) promoter
    Available Since
    March 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    164943-plasmid-map-sequence-id-325844

    Enlarge

  19. pDestTol2-actinb-kcnj13-T131A-EGFP

    Plasmid
    #164942
    Purpose
    Tol2 construct: actinb (actb2) promoter driven zebrafish loss-of-function kcnj13 mutant, T131A and G133R
    Depositor
    GuangJun Zhang
    Article
    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi
    Insert
    kcnj13 (kcnj13 Zebrafish)
    Use
    Tol2 transposon destination vector
    Tags
    EGFP
    Mutation
    Stop codon deleted; T131A; G133R
    Promoter
    actinb (actb2) promoter
    Available Since
    March 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    164942-plasmid-map-sequence-id-325785

    Enlarge

Showing: 7701 - 7720 of 8587 results