We narrowed to 82,246 results for: cloning
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Plasmid#208719PurposeExpresses the red 5-HT sensor GRAB_r5-HT1.0 in neuronsDepositorInsertRed fluorescent 5-HT sensor GRAB_r5-HT1.0
UseAAVPromoterhSynAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG1159 TOM20-ECFP-FIREmate
Plasmid#196693PurposeExpresses TOM20-ECFP-FIREmate in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTOM20-ECFP-FIREmate
TagsFIREmateExpressionMammalianAvailable sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pM-CBE
Plasmid#196293Purpose35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding CBE in place of viral GPDepositorInsertFull length TSWV M antigenome encoding CBE in place of viral GP
UseCRISPRTags3×flagExpressionPlantPromoterduplicated cauliflower mosaic virus 35S promoterAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAXY0002
Plasmid#179835PurposeBinary plant vector expressing eYGFPuv and GUS, both driven by a 35S promoterDepositorTypeEmpty backboneUseBinary vector for plant transformationExpressionPlantAvailable sinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
Halo-Rab6A
Plasmid#190171PurposeExpresses HaloTag-Rab6A in mammalian cells.DepositorAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HRV-B3 IRES
Plasmid#189969PurposePart 2 plasmid to introduce the HRV-B3 IRES into circRNAsDepositorInsertHRV-B3 IRES
UseCloningPromoterN/AAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
iRFP-H2A
Plasmid#158691PurposeThis plasmid encodes an iRFP fused to H2A histone nuclear marker. It has CMV promoter driving the expression of iRFP fused to H2A. It has no selection markersDepositorInsertiRFP670
UseLentiviralExpressionMammalianPromoterCMVAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-2P-OMICRON
Plasmid#180593PurposeMammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSpike (S-GSAS-2P-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
TagsHRV3C cleavage site, 8X His tag, 2X Strep-Tag IIExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…Available sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMPRAv3:minP-GFP
Plasmid#109036PurposeMPRA v3 minimal promoter with EmGFP donorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertemGFP
ExpressionMammalianPromoterminPAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA5/FRT/TO_IgKchL_HA_HaloTag9_myc_PDGFRtmb
Plasmid#175544PurposeExpression of HaloTag9 at the outer leaflet of the plasma membrane of mammalian cellsDepositorInsertIgKchL_HA-HaloTag9-myc_PDGFRtmb
ExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLQ7334: pHR-PGK-SV40_NLS-dCas12f-VPR-c- Myc_NLS-mCherry-WPRE
Plasmid#176268PurposeExpression of dCas12f-VPR-mCherry in mammalian cellsDepositorInsertdCas12f
UseLentiviralTagsHA-VPR-mCherryExpressionMammalianMutationD326A, D510APromoterPGKAvailable sinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBABE-MCS(extended)-Puro (empty vector)
Plasmid#172603PurposeEmpty retroviral vector for the constitutive, near-physiological expression of a gene of interest and a puromycin resistance marker in mammalian cells.DepositorTypeEmpty backboneUseRetroviralExpressionMammalianMutationThe multiple cloning site (MCS) has been edited/e…Available sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV x EF1a_EKAREN5-ires-puro
Plasmid#167821PurposeOptimized EKAREV FRET biosensor sensor for ERKDepositorInsertEKAREN5
UseLentiviralTagsnls localization motifExpressionMammalianMutationK424P, K426WPromoterEF1aAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SEP-GluA2 TKIT
Plasmid#169442PurposeExpression of 2 guides + donor DNADepositorInsertSuper ecliptic pHluorin (SEP)
UseAAV and CRISPRAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLV-MPAct-mRuby3-IRES-Puro
Plasmid#155231Purposelentiviral MPAct constructDepositorInsertLentiviral expression of MPAct-mRuby sensor
UseLentiviralTagsmRuby3Available sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
iOn-CAG∞RFP
Plasmid#154014PurposeVector based on the iOn integration-coupled transcriptional switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposaseDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmRFP1
ExpressionMammalianPromoterCAGAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO/GFP-GIGYF2
Plasmid#141189PurposeExpresses GFP-GIGYF2 in mammalian cells, can be used to make inducible cell lineDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGIGYF2 (GIGYF2 Human)
TagsGFPExpressionMammalianPromoterCMVAvailable sinceJune 5, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSC101-pT7-T3RNAP
Plasmid#140872PurposeT7 promoter driven synthesis of T3 RNA polymeraseDepositorInsertT3 RNA polymerase
ExpressionBacterialPromoterT7 promoterAvailable sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
nCas9-PPE
Plasmid#140445PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertnCas9(H840A)-M-MLV
UseCRISPRExpressionPlantMutationH840A for Cas9; D200N, T306K, W313F, T330P and L…Promotermaize Ubiquitin-1Available sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUC-AdEvolve-DEST
Plasmid#131692PurposeA version of AdEvolve-DEST with a high copy origin for easier DNA preparation and transfection.DepositorTypeEmpty backboneUseAdenoviralAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by