We narrowed to 63,049 results for: cloning

Showing: 7721 - 7740 of 63049 results

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pFH81

Plasmid

#128192

Purpose

Level1 ZmUBIp::ScCas9 (wheat codon optimized):NOSt

Depositor

Vladimir Nekrasov

Publication

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

ZmUBIp::ScCas9 (wheat codon optimized):NOSt

Use

CRISPR and Synthetic Biology

Expression

Plant

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFH47

Plasmid

#125812

Purpose

Level0 LbCas12a, human codon optimized

Depositor

Vladimir Nekrasov

Publication

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

LbCas12a, human codon optimized

Use

CRISPR and Synthetic Biology

Expression

Plant

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFH76

Plasmid

#125815

Purpose

Level0 ScCas9, wheat codon optimized

Depositor

Vladimir Nekrasov

Publication

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

ScCas9, wheat codon optimized

Use

CRISPR and Synthetic Biology

Expression

Plant

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFH15

Plasmid

#125800

Purpose

Level0 StCas9, Arabidopsis codon optimized

Depositor

Vladimir Nekrasov

Publication

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

SaCas9, Arabidopsis codon optimized

Use

CRISPR and Synthetic Biology

Expression

Plant

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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Human NCKX4-AS-short (pcDNA3.1+)

Plasmid

#75210

Purpose

Mammalian expression of SLC24A4

Depositor

Jonathan Lytton

Publication

Li et al J Biol Chem. 2002 Dec 13;277(50):48410-7

Insert

SLC24A4 (SLC24A4 Human)

Expression

Mammalian

Mutation

alternatively spliced exon absent

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pGGW_SM_BamHI

Plasmid

#49315

Purpose

Subcloning vector for Golden Gate-based site-directed mutagenesis; BamHI and KpnI to release the insert

Depositor

Joachim Wittbrodt

Publication

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pGGEV_+1_Linker

Plasmid

#49280

Purpose

Golden Gate entry vector at position 1,small linker insert, plus 5' ATG

Depositor

Joachim Wittbrodt

Publication

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pGGEV_3'_Linker

Plasmid

#49289

Purpose

Golden Gate entry vector at position 3, small linker insert, final entry vector for the assembly

Depositor

Joachim Wittbrodt

Publication

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pGGEV_5'_Linker

Plasmid

#49291

Purpose

Golden Gate entry vector at position 5, small linker insert, final entry vector for the assembly

Depositor

Joachim Wittbrodt

Publication

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pICH87644

Plasmid

#50274

Depositor

Sylvestre Marillonnet, Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter, act2 (AT3G18780, A. thaliana) and 5'UTR, omega (Tobacco Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

March 7, 2014

Availability

Academic Institutions and Nonprofits only

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pICH77901

Plasmid

#50341

Depositor

Sylvestre Marillonnet

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

3'UTR, polyadenylation signal/terminator, mas (A. tumefaciens)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH77911

Plasmid

#50342

Depositor

Sylvestre Marillonnet, Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

3'UTR, polyadenylation signal/terminator, ags (A. tumefaciens)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH71431

Plasmid

#50344

Depositor

Sylvestre Marillonnet, Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

3'UTR, polyadenylation signal/terminator, ATPase (S. lycopersicum )

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL50013

Plasmid

#50312

Depositor

Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

C terminal T7 tag (bacteriophage T7 gene 10)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL50016

Plasmid

#50315

Depositor

Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, variant of turboGFP codon-optimised for plants (P. plumata) with intron, U5 small nuclear ribonucleoprotein component (A.

Use

Plant expression

Mutation

Codon-optimised for A. thaliana. BsaI/BbsI sites …

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH41531

Plasmid

#50321

Depositor

Sylvestre Marillonnet, Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, GFP (A. victoria)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH49477

Plasmid

#50324

Depositor

Sylvestre Marillonnet, Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, DsRed variant of RFP (Discosoma sp.)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH44222

Plasmid

#50287

Depositor

Sylvestre Marillonnet, Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR CMV1 (Cucumber Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH44188

Plasmid

#50289

Depositor

Sylvestre Marillonnet, Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR BSMV (Barley Stripe Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pAGM1479

Plasmid

#50291

Depositor

Sylvestre Marillonnet

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR, omega (Tobacco Mosaic Virus) + N terminal HIS tag (6x polyhistidine)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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