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AiP1417 - pAAV-AiE2051m_3xC2-minBG-SYFP2-WPRE-BGHpA

Plasmid

#220650

Purpose

AiE2051m_3xC2 is an optimized enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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AiP1357 - pAAV-AiE2128m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#220643

Purpose

AiE2128m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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AiP20013 - pAAV-AiE2674m-minBG-SYFP2-WPRE3-BGHpA

Plasmid

#230556

Purpose

AiE2674m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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p5E-EF1a (JDW 1164)

Plasmid

#224473

Purpose

Gateway compatible 5' entry clone containing the human EF1a promoter

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EF1a promoter

Expression

Mammalian

Available since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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p5E TRE-3GV (JDW 1163)

Plasmid

#224476

Purpose

Gateway compatible 5' entry clone containing a 3rd generation Tet-On promoter

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

TRE3GV promoter

Use

Gateway Cloning

Available since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-WPRE-SV40-pA (JDW 922)

Plasmid

#224545

Purpose

A Gateway compatible 3' entry clone containing a woodchuck herpes simplex virus regulatory element (WPRE) to stabilize mRNA upstream of an SV40 polyA

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

WPRE-SV40-pA

Use

Gateway Cloning

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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AiP1307 - pAAV-AiE2016m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214481

Purpose

AiE2016m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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LT3GEPIR-NEK2shRNA1

Plasmid

#220540

Purpose

To inducibly knockdown NEK2 expression

Depositor

Sean Lee

Insert

NEK2 shRNA1 (NEK2 Human)

Use

Lentiviral

Promoter

TRE3G (TetOP) promoter

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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AiP1657 - pAAV-AiE2339m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214508

Purpose

AiE2339m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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AiP1969 - pAAV-AiE2563m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214597

Purpose

AiE2563m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pLX304 SLC2A1

Plasmid

#193691

Purpose

Constitutive lentiviral expression of SLC2A1

Depositor

William Hahn

Publication

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

SLC2A1 (SLC2A1 Human)

Use

Lentiviral

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pGW011b Dual AAV vector pAAV-EFS-dCAS9-spA

Plasmid

#192160

Purpose

Dual AAV vector AAV-dCas9

Depositor

Sidi Chen

Publication

Wang et al Nat Immunol. 2019 Nov;20(11):1494-1505.

Insert

Dead Cas9

Use

AAV

Mutation

NA

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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1137=Hsp70Bb-Cas9[dsRed]

Plasmid

#149424

Purpose

The piggyBac plasmid harboring Hsp70Bb-Cas9-T2A-eGFP-p10 and Opie2-dsRed-SV10 (marker gene)

Depositor

Omar Akbari

Publication

Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

Insert

Cas9

Use

CRISPR

Tags

eGFP

Expression

Insect

Promoter

Dmel Hsp70Bb

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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IDG_TMEM38B_OE_1

Plasmid

#161658

Purpose

Overexpresses TMEM38B

Depositor

Mike McManus

Insert

TMEM38B (TMEM38B Human)

Tags

V5

Expression

Mammalian

Available since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pInducer10-UBC9-sh4

Plasmid

#168988

Purpose

Expresses RFP cDNA and UBC9 shRNA 4 in mammalian cells

Depositor

Ji Luo

Publication

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Ubiquitin Conjugating Enzyme 9

Expression

Mammalian

Promoter

Ubc promoter

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCDF-NtXNtTcR1AtR2NtB2

Plasmid

#160889

Purpose

Expression of N. tabacum Rubisco small subunit (rbcS-Tc), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

Depositor

Maureen Hanson

Publication

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

PT7-Nt-rbcS-Tc

Expression

Bacterial

Promoter

rbcS-Tc: T7, chaperones: T12

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-PIG-METTL14-WT

Plasmid

#141118

Purpose

Retroviral overexpression of WT METTL14 in mammalian cells.

Depositor

Jianjun Chen

Publication

Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

Insert

METTL14 (METTL14 Human)

Use

Retroviral

Expression

Mammalian

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-RABIF

Plasmid

#130882

Purpose

Expresses human RABIF with N-terminal GFP tag

Depositor

Michael Bassik

Publication

Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

Insert

RABIF (RABIF Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-RAB10 K154A

Plasmid

#130886

Purpose

Expresses human RAB10 K154A mutant with N-terminal GFP tag

Depositor

Michael Bassik

Publication

Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

Insert

RAB10 (RAB10 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

K154A

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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negAKARet-cyto

Plasmid

#114733

Purpose

Negative control of AKARet-cyto

Depositor

Ryohei Yasuda

Publication

Tang et al Neuron. 2017 Mar 22;93(6):1315-1324.e3.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Negative Control of AKARet-cyto

Tags

EGFP and sREAChet

Expression

Mammalian

Promoter

CMV

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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