We narrowed to 65,841 results for: cell

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pMPRA2-CMV-Clover

Plasmid

#53539

Purpose

Fluorescent reporter control for pMPRA2

Depositor

Tarjei Mikkelsen

Article

Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

Insert

Clover GFP

Expression

Mammalian

Promoter

CMV IE

Available Since

May 20, 2014

Availability

Academic Institutions and Nonprofits only

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pMPRA3-CMV-Clover

Plasmid

#53540

Purpose

Fluorescent reporter control for pMPRA3

Depositor

Tarjei Mikkelsen

Article

Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

Insert

Clover GFP

Expression

Mammalian

Promoter

CMV IE

Available Since

May 20, 2014

Availability

Academic Institutions and Nonprofits only

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pmCherry-U6-empty

Plasmid

#140580

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Type

Empty backbone

Use

CRISPR

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pRRL-MHCK7-R-GECO

Plasmid

#109374

Purpose

Expresses calcium indicator R-GECO in muscle cells

Depositor

Nenad Bursac

Article

Rao et al Nat Commun. 2018 Jan 9;9(1):126. doi: 10

Insert

R-GECO

Use

Lentiviral

Expression

Mammalian

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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AbVec2.1-IGLC2-MscI

Plasmid

#80797

Purpose

Expression of immunoglobulin light chains in mammalian cells, human lambda 2 constant region

Depositor

Hedda Wardemann

Article

Tiller et al J Immunol Methods. 2009 Oct 31;350(1-2):

Type

Empty backbone

Expression

Mammalian

Promoter

hCMV IE1 gene promoter

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pRSFDuet1-sgRNA(siteA)

Plasmid

#154831

Purpose

sgRNA for CRISPR/Cas9-mediated targeted integration into genomic site A in CHO cells

Depositor

Lise Marie Grav

Article

Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

Insert

sgRNA(siteA)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pJC7

Plasmid

#177872

Purpose

Co-express in mammalian cells two target proteins, using an internal ribosome entry site and two multi-cloning sites.

Depositor

Roberto Dominguez

Article

Carman et al J Biol Chem. 2021 Oct;297(4):101154. doi

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pSNAP-macroH2A1.2 Zeo

Plasmid

#132428

Purpose

Expressing SNAP-tagged histone macroH2A1.2 in mammalian cells

Depositor

John M. Greally

Article

Sato et al J Cell Biol. 2019 Jun 3;218(6):1810-1823

Insert

macroH2A1.2

Expression

Mammalian

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-survivin

Plasmid

#196908

Purpose

visualization of midzone on anaphase and midbody later during mitosis in living mammalian cells

Depositor

Kenji Sugimoto

Article

Fukada et al Biotechniques. 2004 Oct;37(4):552, 554,

Insert

survivin

Tags

EGFP

Expression

Mammalian

Mutation

E129K

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAG244 iFAST

Plasmid

#130809

Purpose

Expresses iFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Biochemistry. 2018 Oct 2;57(39):5648-565

Insert

iFAST

Tags

His-tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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Hook1-EGFP

Plasmid

#171941

Purpose

Expresses Hook1 in mammalian cells

Depositor

Cheng-han Yu

Article

Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

Hook1

Tags

EGFP

Expression

Mammalian

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-Rab9a

Plasmid

#169071

Purpose

Express human Rab9a in mammalian cells

Depositor

Gia Voeltz

Article

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Insert

Rab9a (RAB9A Human)

Tags

acGFP1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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zCREST3:redCas9; control_gRNA

Plasmid

#199334

Purpose

pDEL157; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous control sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

zCREST3
Cas9
control sgRNA

Use

Tol2 destination vector

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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SOX9-T2A-NLS-tdTomato-PGK-Puro

Plasmid

#89993

Purpose

Donor template for generation of SOX9-ntdTomato reporter cell lines

Depositor

Timo Otonkoski

Insert

SOX9-T2A-NLS-TdTomato-PGK-Puro (SOX9 Human)

Use

CRISPR

Promoter

none

Available Since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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pci-EGFP-UPF1-DEAA

Plasmid

#135996

Purpose

DEAA UPF1 inserted with GFP tagged on the N-terminus used to stably integrate into cells

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF1

Tags

EGFP

Expression

Mammalian

Mutation

DE647AA

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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mScarlet-Rab9aS21N

Plasmid

#169073

Purpose

Express human dominant negative form of Rab9a(S21N) in mammalian cells

Depositor

Gia Voeltz

Article

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Insert

Rab9a (RAB9A Human)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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p-miniTurbo-HA

Plasmid

#209637

Purpose

Expression miniTurbo-HA in mammalian cells

Depositor

Xiang Zhou

Article

Lu et al Nucleic Acids Res. 2024 Mar 7:gkae126. d

Insert

miniTurbo

Expression

Mammalian

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pTol2_LSEC:Cas9green; erbb2_gRNA171

Plasmid

#199338

Purpose

pDEL135; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous erbb2 sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

LSEC
Cas9
erbb2 sgRNA

Use

Tol2 destination vector

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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poRFP-oGFP

Plasmid

#154130

Purpose

Expresses orthogonal gfp and orthogonal rfp genes in bacterial cells

Depositor

Alexander Mankin

Article

Aleksashin et al Nat Commun. 2020 Apr 20;11(1):1858. doi:

Inserts

orthogonal gfp
orthogonal rfp

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 27, 2021

Availability

Academic Institutions and Nonprofits only

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pCZGY2849

Plasmid

#74112

Purpose

Enhanced mutant version of membrane targeted PH-miniSOG (Q103L) entry clone for more efficient ablation of cells when expressed under desired promoter.

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

miniSOG(Q103L)

Use

Gateway topo cloning

Tags

PH

Available Since

April 8, 2016

Availability

Academic Institutions and Nonprofits only

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