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LLP772_L2pV1_NOR_B3RT_FRT_Rluc_2IBF_lacZ

Plasmid

#192432

Purpose

To act as a 2-input state of the NOR gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-B3RT-Rluc-B3RT-FRT

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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IRE-nonOpt-TREAT

Plasmid

#196922

Purpose

The codon optimized Renilla coding sequence in the IRE-TREAT reporter was replaced by non-optimal Renilla containing 93 synonymous codon substitutions.

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

non-optimal Renilla luciferase

Expression

Bacterial and Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pminiT-MED6-Halo donor

Plasmid

#197866

Purpose

Donor plasmid used for building MED6-Halo knock-in mESCs

Depositor

James Zhe Liu

Article

Dong et al bioRxiv 2022.10.26.513917

Insert

Med6 (Med6 Mouse)

Use

Mouse Targeting

Tags

Halotag

Promoter

NA

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hAIM2deltPYD-T7

Plasmid

#51539

Purpose

Express T7-tagged HIN domain of hAIM2 in mammalian cells

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Tags

T7

Expression

Mammalian

Mutation

deletion of pyrin domain

Promoter

CMV

Available Since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

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pPJ174(JPUB_006779)

Plasmid

#83827

Purpose

Carries operon 1 final

Depositor

Harry Beller

Article

Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

Inserts

Kuste3603
kuste3605
kuste3606

Use

Pfab217 (p15a ori, kanr)

Promoter

op1_3603_for, op1_3605_for, and op1_3606_for

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pBAD-BirA-eCPX-AP(K10)

Plasmid

#121908

Purpose

Expresses BirA and eCPX-AP(K10)

Depositor

Per Svenningsen

Article

Granhoj et al Sci Rep. 2019 Mar 11;9(1):4118. doi: 10.

Inserts

BirA (birA Synthetic)
enhanced Circularly Permuted Outer Membrane Protein X

Tags

6xHis

Expression

Bacterial

Mutation

changed lysine 10 in the AP to alanine

Promoter

T7 and pBAD promoter

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pBEAST2-sfGFP

Plasmid

#126575

Purpose

Strong constitutive expression of super-folder GFP for cell-free expression

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

sfGFP

Use

Synthetic Biology; Cell-free protein synthesis

Available Since

June 19, 2019

Availability

Academic Institutions and Nonprofits only

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PcDNA-DEST47-CypB-Iso C-term GFP

Plasmid

#36130

Depositor

Patrick Van Oostveldt

Article

Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

Insert

cyclophilin B (PPIB Human)

Tags

GFP

Expression

Mammalian

Mutation

contains amino acids 47-204 of cyclophilin B

Promoter

CMV

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
PcDNA-DEST47-CypB-Golgi C-term GFP

Plasmid

#36128

Depositor

Patrick Van Oostveldt

Article

Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

Insert

cyclophilin B (PPIB Human)

Tags

GFP

Expression

Mammalian

Mutation

contains amino acids 1-211 of cyclophilin B

Promoter

CMV

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
SERA5-bio-His

Plasmid

#50817

Purpose

Expresses enzymatically monobiotinylated full length SERA5

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

SERA5

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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PcDNA-DEST53-CypB-Golgi N-term GFP

Plasmid

#36127

Depositor

Patrick Van Oostveldt

Article

Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

Insert

cyclophilin B (PPIB Human)

Tags

GFP

Expression

Mammalian

Mutation

contains amino acids 1-211 of cyclophilin B

Promoter

CMV

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
pET28a-BCA-Pep-H

Plasmid

#185782

Purpose

Encodes enzyme bovine carbonic anhydrase linked to Pep-H self-assembling peptide via a GS-linker sequence

Depositor

Lizhong He

Article

Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

Insert

BCA-Pep-H

Expression

Bacterial

Promoter

T7

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pET29b_Qcat5

Plasmid

#45133

Depositor

Christopher Petzold

Article

Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

Insert

E-coli-pathways-QconCAT5

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pET29b_Qcat2

Plasmid

#45130

Depositor

Christopher Petzold

Article

Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

Insert

E-coli-pathways-QconCAT2

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Dec. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pET29b_Qcat6

Plasmid

#45134

Depositor

Christopher Petzold

Article

Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

Insert

E-coli-pathways-QconCAT6

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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p3E-SGTAP no-pA

Plasmid

#82599

Purpose

3' entry vector containing streptavidin binding protein (SBP) followed by a TEV cleavage site and 2 protein G copies without pA for tandem affinity purification

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

SBP-TEV-2x protein G

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pSMP-Suv39H2_3

Plasmid

#36346

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Suv39H2 (SUV39H2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pMars1CyeGFP

Plasmid

#75222

Purpose

Mammalian intracellular expression of fluorogen activating protein Mars1Cy-eGFP fusion

Depositor

Alan Waggoner

Article

Zhang et al J Clin Invest. 2015 Oct 1;125(10):3915-2

Insert

Mars1Cy-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2016

Availability

Academic Institutions and Nonprofits only

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pPI180

Plasmid

#113222

Purpose

generic knockin vector for C-terminal tagging with GFP

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Generic knockin vector for c-terminal tagging wit…

Tags

GFP

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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Split-RESCUE-N

Plasmid

#170153

Purpose

AAV vector carrying the MCP-RESCUE-DDN (RESCUE is the evolved ADAR2 variant for C-U editing) with a nuclear export signal

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

NES-MCP-RESCUE-DDN

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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