We narrowed to 8,732 results for: Set

1. pGEM-T-Easy-NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT

   Plasmid

   #119067

   Purpose

   Donor construct to amplify NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT cassette for BAC recombination

   Depositor

   Tatjana Sauka-Spengler

   Article

   Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

   Insert

   NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT
   

   Expression

   Bacterial

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJW1846

   Plasmid

   #154326

   Purpose

   Multi-cassette for SapTrap

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   30aa linker::GFP^SEC^AID*::3xFLAG
   

   Use

   CRISPR and Cre/Lox

   Expression

   Worm

   Mutation

   F+E mutations in sgRNA

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. YW249_Pzipt17-loxP-H2BGFP-miniMOS

   Plasmid

   #196063

   Purpose

   a plasmid modified from miniMOS vector pCFJ910. the modification including insertions of SL2::H2B::GFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and GFP cassettes.

   Depositor

   Wenxing Yang

   Article

   Li et al PeerJ. 2023 May 17;11:e15433. doi: 10.77

   Inserts

   H2B::GFP
   NeoR
   Minimal Mos1
   zipt17p::zipt17::zipt17 3UTR
   

   Expression

   Worm

   Available Since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEM-T-Easy-HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT

   Plasmid

   #119065

   Purpose

   Donor construct to amplify HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT cassette for BAC recombination

   Depositor

   Tatjana Sauka-Spengler

   Article

   Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

   Insert

   HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT
   

   Tags

   HA

   Expression

   Bacterial

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. ku70 insUP+pPGI+NAT+tNOS+ku70 insD (SBE217)

   Plasmid

   #195049

   Purpose

   integration cassette for ku70 deletion and expression of NAT gene under pPGI for characterization

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   NAT
   

   Expression

   Bacterial

   Promoter

   pPGI

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. qTAG-C-mNeon-Blast-TUBB4B

   Plasmid

   #207786

   Purpose

   Donor template for mNeon-2A-Blast insertion into the C-terminus of the TUBB4B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBB4B Addgene #207785

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TUBB4B Homology Arms flanking a mNeon-Blast Cassette (TUBB4B Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. AA290

   Plasmid

   #215949

   Purpose

   Fragmid fragment: (guide cassette) guide expression; positive control cell surface

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U6_v1; sgCD55_v4; trRNA_v4 [Sp]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. AA286

   Plasmid

   #215948

   Purpose

   Fragmid fragment: (guide cassette) guide expression; positive control cell surface

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U6_v1; sgCD47_v6; trRNA_v4 [Sp]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pv9_CBX7_2xChromo-13XL-BASU

   Plasmid

   #179413

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7.2xChromo-13XL-BASU

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   CBX7.2xChromo-13XL-BASU
   

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAS_4xG1 PylT FLAG-G1 PylRS

    Plasmid

    #154768

    Purpose

    plasmid with 4xG1 PylT cassette and G1 PylRS for amber suppression in transient or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-short Cre-on G/W

    Plasmid

    #86949

    Purpose

    Gateway destination transfer plasmid for making AAV. Contains a DIO cassette for cre-induction. For introducing large constructs into AAV

    Depositor

    Joseph Dougherty, Rob Mitra

    Article

    Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Available Since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. sgRNA1_IL1RN

    Plasmid

    #64140

    Purpose

    Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    IL1RN (IL1RN Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT2/CMV-eGFP.PB/PTK

    Plasmid

    #170540

    Purpose

    Expresses the puromycin resistance gene fused to a thymidine kinase, upon excision of this cassette the reading frame of eGFP is restored

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

    Insert

    Delta 5'-eGFP CMV-PuroTK- Delta 3'-eGFP
    

    Expression

    Mammalian

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRI018-pKW20088-PelcA-mCherry-TtrpC-PU3-E1-Cas9Scaffold-8xT

    Plasmid

    #140205

    Purpose

    Proof-of-concept of CRISPR/dSpCas9-VPR activation, encoding PelcA-mCherry along with the sgRNA E1 expression cassette, targeting PelcA.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Inserts

    mCherry
    sgRNA E1
    

    Use

    CRISPR and Synthetic Biology; Proof-of-concept te…

    Mutation

    G174D

    Promoter

    PelcA and U3

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. PV225

    Plasmid

    #132334

    Purpose

    dCas9 plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea mays

    Depositor

    Joshua Young

    Article

    Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

    Insert

    dCas9-AT-CBF1 (CBF1 Cas9 (Streptococcus pyogenes); AT-CBF1 (Arabidopsis thaliana))
    

    Tags

    AT-CBF1

    Expression

    Plant

    Mutation

    Codon optimize for expression and stability in Ze…

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. sgRNA3_IL1RN

    Plasmid

    #64151

    Purpose

    Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA3_IL1RN (IL1RN Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. qTAG-C-moxGFP-Puro-H2BC11-MMEJ

    Plasmid

    #207760

    Purpose

    MMEJ donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H2BC11 Short Homology Arms flanking a moxGFP-Puro Cassette (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pv9_MBD1_MBD-WT-13XL-BASU

    Plasmid

    #179416

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1.MBD-WT-13XL-BASU

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    MBD1.MBD-WT-13XL-BASU
    

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. Direct-seq_lentiGuide-Puro-tRNA-Tail-8A8G

    Plasmid

    #157984

    Purpose

    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

    Depositor

    Lijia Ma

    Article

    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

    Insert

    S. pyogenes sgRNA cassette (tRNA-Tail-8A8G)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Direct-seq_lentiGuide-Puro-tRNA-Tetra Loop-8A8G

    Plasmid

    #157985

    Purpose

    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

    Depositor

    Lijia Ma

    Article

    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

    Insert

    S. pyogenes sgRNA cassette (tRNA-Tetra-8A8G)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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