We narrowed to 61,408 results for: Ide

1. pET29b-mVenus-dspB(E184Q)-6xHis

   Plasmid

   #176576

   Purpose

   Plasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

   Depositor

   Mark Nitz

   Article

   Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

   Insert

   Dispersin B
   

   Tags

   Hexahistidine tag and mVenus

   Expression

   Bacterial

   Mutation

   E184Q

   Promoter

   T7

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPI278

   Plasmid

   #113227

   Purpose

   extrachromosomal expression vector, expression of H2B-GFP

   Depositor

   Rob Kay

   Article

   Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

   Insert

   H2B
   

   Use

   Dictyostelium discoideum expression vector, extra…

   Tags

   GFP

   Promoter

   act15

   Available Since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBAD-TRP120

   Plasmid

   #71732

   Purpose

   inducible expression of TRP120 in E. coli

   Depositor

   Jere McBride

   Article

   Luo et al Clin Vaccine Immunol. 2009 Jul;16(7):982

   Insert

   TRP120
   

   Tags

   6xHIS and Thioredoxin

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pC2xTC1-alphaTubulin

   Plasmid

   #36326

   Depositor

   Amy Palmer

   Article

   Van Engelenburg et al Chembiochem. 2010 Mar 1;11(4):489-93.

   Insert

   alpha tubulin 1b with tetracysteine peptides for ReAsH labeling (TUBA1B Human, Synthetic)
   

   Tags

   2xTC1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. Dapp1-PH pET11a

   Plasmid

   #71376

   Purpose

   Phosphoinositide Binding

   Depositor

   Mark Lemmon

   Article

   Ferguson et al Mol Cell. 2000 Aug;6(2):373-84.

   Insert

   Dapp1-PH (DAPP1 Human)
   

   Expression

   Bacterial

   Mutation

   Pleckstrin homology (PH) domain; aa 148-273

   Promoter

   T7

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

6. PB-Tal1

   Plasmid

   #60670

   Purpose

   PiggyBac expression vector encoding murine Tal1 downstream of a doxycycline inducible promoter

   Depositor

   James Thomson

   Article

   Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

   Insert

   Tal1 (Tal1 Mouse)
   

   Use

   Piggybac

   Expression

   Mammalian

   Available Since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPI452

   Plasmid

   #113203

   Purpose

   Inducible expression vector (dox-on) N-terminal mScarlet tagging

   Depositor

   Rob Kay

   Article

   Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

   Type

   Empty backbone

   Use

   Dictyostelium discoideum inducible expression vec…

   Tags

   mScarlet

   Available Since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Grp1-PH pET11a

   Plasmid

   #71377

   Purpose

   Phosphoinositide Binding

   Depositor

   Mark Lemmon

   Article

   Ferguson et al Mol Cell. 2000 Aug;6(2):373-84.

   Insert

   Grp1-PH (Cyth3 Mouse)
   

   Expression

   Bacterial

   Mutation

   Pleckstrin homology (PH) domain; aa 264-391

   Promoter

   T7

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pDM1082

   Plasmid

   #108982

   Purpose

   Knockout cassette G418

   Depositor

   Rob Kay

   Article

   Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

   Insert

   Knockout cassette G418
   

   Use

   Cre/Lox; Dictyostelium discoideum knockout vector

   Promoter

   coaA

   Available Since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCORE-Hp53

    Plasmid

    #72242

    Purpose

    Contains the CORE cassette with hyg and human p53 gene markers

    Depositor

    Michael Resnick, Francesca Storici

    Article

    Storici et al Genet Eng (N Y). 2003;25:189-207.

    Inserts

    p53 (TP53 Human)
    hyg
    

    Expression

    Bacterial

    Mutation

    V122A mutation

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPEE17

    Plasmid

    #128361

    Purpose

    Detecting gene essentiality in Cryptococcus neoformans. Targets DNA constructs to the Safe Haven Site 1, a small gene-free region. Contains amdS2 counterselectable marker. This version of the Safe Haven vector includes direct repeats, enabling the formation of popouts on fluoroacetamide.

    Depositor

    James Fraser

    Article

    Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324

    Insert

    Acetamidase (amdS)
    

    Use

    Unspecified

    Promoter

    TEF1

    Available Since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDYU1G/mRuby3–histone H1; γ-tubulin-mEGFP

    Plasmid

    #154303

    Purpose

    Expresses mRuby3–histone H1 and γ-tubulin-mEGFP from actin15 promoter in Dictyostelium discoideum

    Depositor

    Shigehiko Yumura

    Article

    Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

    Inserts

    histone H1
    γ-tubulin
    

    Use

    Dictyostelium expression

    Tags

    mEGFP and mRuby3

    Promoter

    actin15

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Syt1LeftArm-3XGS-GCaMP6s-SV40-3XP3-dsRed-SV40-Syt1RightArm

    Plasmid

    #159636

    Purpose

    Donor plasmid for generating Syt1:GCaMP6s strain in Aedes aegypti with CRISPR/Cas9

    Depositor

    Carolyn McBride

    Article

    Zhao et al Cell Rep Methods. 2021 Jul 26;1(3):10004

    Insert

    GCaMP6s
    

    Expression

    Insect

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. lentiCRISPR - DDX3Y sgRNA 2

    Plasmid

    #70657

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against DDX3Y
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

15. lentiCRISPR - DDX3Y sgRNA 1

    Plasmid

    #70656

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against DDX3Y
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

16. PB-Pitx2

    Plasmid

    #60668

    Purpose

    PiggyBac expression vector encoding murine Pitx2 downstream of a doxycycline inducible promoter

    Depositor

    James Thomson

    Article

    Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

    Insert

    Pitx2 (Pitx2 Mouse)
    

    Use

    Piggybac

    Expression

    Mammalian

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKH-MoHyPer

    Plasmid

    #78267

    Purpose

    Express HyPer-AS sensor that change excitation wavelength with the presence of hydrogen peroxide.

    Depositor

    Jeffrey Caplan, Nicole Donofrio

    Article

    Huang et al Mol Plant Pathol. 2016 Mar 7. doi: 10.11

    Insert

    MoHyper
    

    Use

    Express in magnaporthe

    Expression

    Yeast

    Promoter

    Rp27

    Available Since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pCDH_ss-SBP-EGFP

    Plasmid

    #172358

    Purpose

    Reporter to monitoring a streptavidin-binding peptide trafficking

    Depositor

    Guido Kroemer

    Article

    Zhao et al Sci Rep. 2018 Oct 8;8(1):14966. doi: 10.

    Insert

    ER importing signal sequence
    

    Use

    Lentiviral

    Tags

    SBP-EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. p8126 MSCV-P C-FlagHA 16E7 R77S

    Plasmid

    #163305

    Purpose

    Expresses HPV16 E7 R77S

    Depositor

    Elizabeth White

    Article

    Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

    Insert

    HPV16 E7 R77S
    

    Use

    Retroviral

    Tags

    FlagHA

    Mutation

    R77S

    Promoter

    MSCV LTR

    Available Since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. p6993 MSCV-Neo C-HA 18E7

    Plasmid

    #163311

    Purpose

    Expresses HPV18 E7

    Depositor

    Elizabeth White

    Article

    Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

    Insert

    HPV18 E7
    

    Use

    Retroviral

    Tags

    HA

    Promoter

    MSCV LTR

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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