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pFAB3689

Plasmid

#47834

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 3 + BCD1 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 3 and BCD1

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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Twitch-3 pRSETB

Plasmid

#48204

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-3A

Tags

6xHIS, cpVenusCD, and mCerulean3

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only
pGL3-Enhancer-ZC3H12D_PromoterWT

Plasmid

#153066

Purpose

ZC3H12D promoter region cloned upstream of luciferase reporter gene

Depositor

Silvia Monticelli

Article

Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

Insert

ZC3H12D promoter region (ZC3H12D Human)

Use

Luciferase

Expression

Mammalian

Promoter

none

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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p164-pAAV-mDlx-FLEX-mScarlet

Plasmid

#204689

Purpose

Fluorescent reporter under the control of the Dlx promotor

Depositor

Lynette Lim

Article

Fisher et al Neuron. 2023 Dec 7:S0896-6273(23)00887-5

Insert

mScarlet

Use

AAV and Cre/Lox

Tags

mScarlet palmitoylation

Expression

Mammalian

Promoter

mDlx

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMiR-E6AP-3'UTR

Plasmid

#53694

Purpose

Luciferase reporter assay for microRNA binding on E6AP 3'UTR

Depositor

Edward Chan

Article

Jung et al Mol Cancer. 2014 Apr 8;13(1):80. doi: 10

Insert

UBE3A 3'UTR (UBE3A Human)

Use

Luciferase

Available Since

June 19, 2014

Availability

Academic Institutions and Nonprofits only
HP21-TPI-PTC1

Plasmid

#131043

Purpose

Expresses TPI-PTC1 NMD reporter (It has a Premature termination codon (PTC) at First Amino acid) with a 21 nt hairpin in 5'UTR

Depositor

Marvin Tanenbaum

Article

Hoek et al Mol Cell. 2019 May 23. pii: S1097-2765(1

Insert

TPI-PTC1 (TPI1 Human)

Expression

Mammalian

Mutation

PTC1

Promoter

Pcmv

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAS_4xG1 PylT sfGFP 150TAG

Plasmid

#154767

Purpose

plasmid with 4xG1 PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

sfGFP

Expression

Mammalian

Mutation

150TAG

Promoter

EF1

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-mSncg-0.66kb-EGFP

Plasmid

#153165

Purpose

Truncated mouse gamma-synuclein (mSncg-0.66kb) promoter-mediates gene expression in retinal ganglion cells with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCXLE-dCas9VPP300-T2A-GFP-shP53

Plasmid

#102896

Purpose

EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-P300core followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

dCas9VPP300-T2A-GFP-shP53

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Promoter

CAG

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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E1b-GFP-Tol2-Gateway dre SE-zic2a P

Plasmid

#89385

Purpose

Vector for reporter assay of one zebrafish super-enhancer region

Depositor

Alena Shkumatava

Article

Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

Insert

zic2a (zic2a Zebrafish)

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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pT2-7xTcf-NLS-ONL

Plasmid

#65713

Purpose

Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL)

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

7xTcf, minCMV, 3xNLS, ONL

Use

Luciferase

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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p1.2-Zeo-eGFP

Plasmid

#162743

Purpose

Fluorescent reporter for CHO cells expression studies

Depositor

Ivan Vorobiev

Article

Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

Insert

enhanced green fluorescent protein

Expression

Mammalian

Mutation

consensus Kozak sequence (GCCGCCATGG) added befor…

Promoter

CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pFAB3893

Plasmid

#47844

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 13 + BCD1 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 13 and BCD1

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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E1010m (RFP)_CD

Plasmid

#66033

Purpose

MoClo Basic Part: CDS - Fluorescent protein. Red. Modified from Bba_E1010 to fix illegal sites. [C:E1010m:D]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Fluorescent reporter - RFP

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pTol2CG2-QUASM1:mScarletNLS-SV40pA

Plasmid

#155124

Purpose

Tol2 vector containing QUASM1 reporter element upstream of mScarlet-NLS. Includes cmlc2:mRFP-SV40pA transgenesis marker

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QUASM1:mScarletNLS

Use

Zebrafish expression

Promoter

QUASM1-E1b

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pOPS0380

Plasmid

#133230

Purpose

Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

Depositor

Philip Poole

Article

Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

Insert

constructed with constitutive promoter Rlv3841pnifH (pOGG082), mCherry (EC15071) and T-pharma (pOGG003)

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-Trex2-bpA-EF1BFP

Plasmid

#111144

Purpose

Expression of mouse Trex2 and BFP reporter in mammalian cells

Depositor

Ralf Kuehn

Article

Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

Inserts

mouse Trex2 coding region (Trex2 Mouse)
BFP (Rnf112 )

Expression

Mammalian

Promoter

CAG and EF1

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3-24p3/LCN2-282kB mut-LUC

Plasmid

#46867

Purpose

murine 24p3 minimal promoter with NF-kB mutation as a luciferase reporter

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

24p3/lipocalin 2 proximal promoter

Use

Luciferase

Mutation

NF- kB site is mutated (see comments)

Promoter

24p3 promoter (inactive)

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only
pAS_4xMx PylT C41CA_GFP 150TAG

Plasmid

#140017

Purpose

Plasmid with 4xMxPylT C41CA cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

sfGFP

Expression

Mammalian

Mutation

150TAG

Promoter

EF1

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pOTTC1617 pAAV SYN1 Nuc-EYFP

Plasmid

#135567

Purpose

An AAV vector expressing a neuronally expressed nuclear EYFP reporter

Depositor

Christopher Richie

Article

Venniro et al Proc Natl Acad Sci U S A. 2020 Apr 7;117

Inserts

empty
Nuc-EYFP

Use

AAV

Tags

3xNLS

Expression

Mammalian

Promoter

hSYN1

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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