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M26I DJ-1 TEV site pET15b

Plasmid

#60685

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

Insert

M26I DJ-1 (PARK7 Human)

Tags

6xHis

Expression

Bacterial

Mutation

M26I and TEV site was inserted (see comments belo…

Promoter

T7

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pNAS1b split mCherry NEDD4 WW2

Plasmid

#111882

Purpose

Mode #2 phosphosite expression (split mCherry, using NEDD4 WW2 binding domain)

Depositor

Jesse Rinehart

Article

Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

Type

Empty backbone

Tags

Split mCherry system: NEDD4 WW2 fused to C-termin…

Expression

Bacterial

Available Since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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pRKVSV Rac T17N

Plasmid

#50537

Depositor

Kenneth Yamada

Article

Koivisto et al Exp Cell Res. 2004 Feb 1;293(1):68-80.

Insert

small GTPase, mutated (RAC1 Human)

Tags

2X VSV

Expression

Mammalian

Mutation

T17N (dominant negative)

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pMuLE ENTR SV40 tdTomato L5-L2

Plasmid

#62154

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and tdTomato module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

tdTomato

Use

Mule gateway entry vector

Expression

Mammalian

Promoter

SV40

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pPKm-235

Plasmid

#90509

Purpose

pSIN - EF-1alpha - MTS sHO1 - P2A - MTS - Hy2, vector encoding for mitochondrial-tagged Synechococcus sp. Heme Oxigenase-1 (HO1) and phytochromobilin:ferredoxin oxidoreductase (Hy2)

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

mito-sHO1, mito-Hy2

Use

Lentiviral

Available Since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28b-hTIG3-1-164

Plasmid

#100724

Purpose

Full-length human TIG3 in E.coli expression vector

Depositor

Byung Il Lee

Article

Han et al Protein Expr Purif. 2010 May;71(1):103-7

Insert

RARRES3 (PLAAT4 Human)

Tags

His

Expression

Bacterial

Promoter

T7 promotor

Available Since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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FgH1tUTG_huMcl-1.2

Plasmid

#85531

Purpose

Inducible expression of guide RNA (huMcl-1.2) with fluorescent GFP reporter

Depositor

Marco Herold

Article

Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

Insert

hu Mcl-1.2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

H1t

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf180

Plasmid

#12868

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf180

Expression

Mammalian

Available Since

Dec. 13, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf181

Plasmid

#12869

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf181

Expression

Mammalian

Available Since

Dec. 13, 2006

Availability

Academic Institutions and Nonprofits only
pMuLE ENTR 7SK-miR-30 L5-L2

Plasmid

#62120

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

RNAi; Mule gateway entry vector

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only
pet23a_RBPMS_1_120

Plasmid

#65730

Purpose

plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Tags

His Tag

Expression

Bacterial

Mutation

deletion construct encoding amino acids 1-120

Promoter

T7

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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pWay21-MKP-1DCH2AB

Plasmid

#13472

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1DCH2AB (Dusp1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of MKP-1 CH2AB; contains aa 137-367

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pBS CRABP1

Plasmid

#19189

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Cellular retinoic acid binding protein 1 (CRABP1 Chicken)

Expression

Bacterial

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3-Flag-Tipin-L195A

Plasmid

#23116

Depositor

Aziz Sancar

Article

Kemp et al J Biol Chem. 2010 May 28;285(22):16562-7

Insert

Tipin (TIPIN Human)

Tags

Flag

Expression

Mammalian

Mutation

changed Leucine 195 to Alanine

Available Since

Feb. 8, 2010

Availability

Academic Institutions and Nonprofits only
YFP Gamma 10

Plasmid

#36108

Depositor

Narasimhan Gautam

Article

Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

Insert

G protein subunit Gamma 10 (Gng10 Mouse)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only
pMT3-RhoAC

Plasmid

#100032

Purpose

This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with adenylyl cyclase activity.

Depositor

Daniel Oprian

Article

Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1

Insert

RhoAC

Tags

1D4 (TETSQVAPA)

Expression

Mammalian

Mutation

E497K/C566D

Promoter

Adenovirus major late promoter

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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p177 Dync1i2.C (Ex 1b)

Plasmid

#26445

Depositor

Elizabeth Fisher

Article

Kuta et al PLoS One. 2010 . 5(7):e11682.

Insert

cytoplasmic dynein 1 intermediate chain 2 isoform C (exon 1b) (Dync1i2 Mouse)

Use

Subcloning and sequencing

Mutation

N/A

Available Since

March 24, 2011

Availability

Academic Institutions and Nonprofits only
pP[RS3]3'M

Plasmid

#53552

Purpose

for an easy screen of TALEN- and CRISPR/Cas9- mediated mutagenesis in Drosophila

Depositor

Renjie Jiao

Article

Yu et al Biol Open. 2014 Apr 15;3(4):271-80. doi:

Insert

AscI and MluI restriction enzyme sites

Expression

Insect

Promoter

same pP[RS3]

Available Since

Dec. 3, 2014

Availability

Academic Institutions and Nonprofits only
p196 Dync1i2.A (Ex 1a)

Plasmid

#26446

Depositor

Elizabeth Fisher

Article

Kuta et al PLoS One. 2010 . 5(7):e11682.

Insert

cytoplasmic dynein 1 intermediate chain 2 isoform A (exon 1a) (Dync1i2 Mouse)

Use

Subcloning and sequencing

Mutation

N/A

Available Since

March 24, 2011

Availability

Academic Institutions and Nonprofits only
p169 Dync1i1.C

Plasmid

#26440

Depositor

Elizabeth Fisher

Article

Kuta et al PLoS One. 2010 . 5(7):e11682.

Insert

cytoplasmic dynein 1 intermediate chain 1 isoform C (Dync1i1 Mouse)

Use

Subcloning and sequencing

Mutation

N/A

Available Since

March 21, 2011

Availability

Academic Institutions and Nonprofits only