We narrowed to 16,556 results for: nans
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Plasmid#212123PurposeFor expression of the bacterial gasdermin (bGSDM) encoded by Runella zeae. Protein is expressed with an N-terminal SUMO2 tag.DepositorInsertRunella bGSDM
Tags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available SinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Vitiosangium-bGSDM
Plasmid#212127PurposeFor expression of the bacterial gasdermin (bGSDM) encoded by a Vitiosangium species. Protein is expressed with an N-terminal SUMO2 tag.DepositorInsertVitiosangium bGSDM
Tags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available SinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Gillisia-bGSDM
Plasmid#212128PurposeFor expression of the bacterial gasdermin (bGSDM) encoded by a Gillisia species. Protein is expressed with an N-terminal SUMO2 tag.DepositorInsertGillisia bGSDM
Tags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available SinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUASTattB_NSnoFbox-vhhGFP4
Plasmid#35578DepositorAvailable SinceApril 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHS1516
Plasmid#205979PurposeFLAG-SV40 NLS-Cas7 from MWEE01000013 Cas5-HNH in pcDNA3.1(+)DepositorInsertCas7 from MWEE01000013, Cas5-HNH
TagsFLAG-SV40 NLSExpressionMammalianPromoterCMVAvailable SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
ANKRD24-TwinStrepII
Plasmid#83190PurposeExpression of tagged protein in mammalian cellsDepositorAvailable SinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
MBC1-PuroIII
Plasmid#153419PurposeMSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1) for expression of TCR genesDepositorInsertmurine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1)
UseRetroviralExpressionMammalianAvailable SinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-FAS-ChETA-TdTomato-WPRE-pA
Plasmid#37089PurposeExpresses ChETA-tdTomato in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites), for optogenetic excitationDepositorInsertChETA-TdTomato
UseAAV and Cre/Lox; Cre-offTagsTdTomatoPromoterEF1aAvailable SinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTG-mTDG-K341R
Plasmid#52267PurposeBacterial expression of mouse TDG-K341R with C-terminal GST-tagDepositorInsertTDG (Tdg Mouse)
TagsGSTExpressionBacterialMutationK341R, SUMO acceptor site mutationPromoterT7Available SinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-StefinA
Plasmid#182403PurposeBacterial expression of Vesicle Nucleating peptide-stefinA fusionDepositorInsertVNp-stefin (CSTA Human)
TagsVesicle Nucleating peptide (VNp)ExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-Gαi3-YFP (Citrine variant, b/c loop insert)
Plasmid#171375PurposeFor the mammalian expression of rat Gαi3 internally tagged with YFPDepositorInsertGai3-YFP
TagsYFPExpressionMammalianPromoterCMVAvailable SinceSept. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCI.neo-rPAM1-M314I
Plasmid#145802Purposeexpresses catalytically inactive rat PAM-1 in mammalian cellsDepositorInsertrat PALcc (peptidyl-hydroxyglycine alpha-amidating monooxygenase) (Pam Rat)
ExpressionMammalianMutationchanged methionine 314 to isoleucine to inactivat…Available SinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn S129G
Plasmid#36049DepositorAvailable SinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBbB8k-csg-NbMS2
Plasmid#171701PurposeArabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a nanobody targeting the coat protein of bacteriophage MS2 via a flexible linker.DepositorInsertcsgBACEFG, with nanobody fused to CsgA
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mNRDc-E>A-V5
Plasmid#51298Purposeexpression of the inactive mutant of mouse NRDc in mammalian cellsDepositorInsertNRDc E247A (Nrd1 Mouse)
TagsHIS and V5ExpressionMammalianMutationE247A, enzymatic inactive mutantPromoterCMVAvailable SinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
RhopH3-bio
Plasmid#47781PurposeExpresses enzymatically monobiotinylated full-length RhopH3 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised RhopH3
Tagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable SinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRSET-pr-mEos4b (mEosFP4b-V69T)
Plasmid#99226PurposeBacterial expression of the primed conversion capable protein pr-mEos4b (mEos4b-V69T).DepositorInsertpr-mEos4b
ExpressionBacterialMutationV69T for primed conversion applicationsPromoterT7Available SinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
N-TAP A2A in pcDNA3
Plasmid#67850Purposemammalian expression of A2A receptor with N terminal TAP tagDepositorAvailable SinceOct. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
sg1+sg2+sg3
Plasmid#113969PurposeTriple short guide RNA targeting GTATAGCATACATTATACG, TACCACATTTGTAGAGGTT & CAATGTATCTTATCATGTCDepositorInsertsg1+sg2+sg3
ExpressionMammalianPromoterU6Available SinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
plKO.1-DNAJC5-ShRNA
Plasmid#205729PurposeKnockdown of DNAJC5DepositorInsertshRNA targeting DNAJC5 (DNAJC5 Human)
UseLentiviralAvailable SinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only