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Showing: 121 - 160 of 25904 results

  1. pVITRO-Fab_MTX-LUCID

    Plasmid
    #119047
    Purpose
    Plasmid encoding the mammalian expression of the Metotraxate LUCID based on antibodies (BRET semi-synthetic biosensor)
    Depositor
    Kai Johnsson
    Article
    Xue et al Angew Chem Int Ed Engl. 2017 Jun 12;56(2
    Inserts
    Fab_MTX-LUCID_part1
    Fab_MTX-LUCID_part2
    Use
    Tags
    FLAG-tag, His-tag, and Strep-tag
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α + CMV/SV40 enhancers and IRES
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    119047-plasmid-map-sequence-id-231808

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  3. pET28-AviTag-DogCatcher-MBP

    Plasmid
    #171928
    Purpose
    Expresses AviTag-DogCatcher-MBP in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.
    Depositor
    Mark Howarth
    Article
    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-
    Insert
    AviTag-DogCatcher-MBP
    Use
    Tags
    His6 and Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    DogCatcher added to N-terminus of MBP via a GSGES…
    Promoter
    T7
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171928-plasmid-map-sequence-id-338570

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  6. pET28-AviTag-DogTag-MBP

    Plasmid
    #171773
    Purpose
    Expresses AviTag-DogTag-Maltose binding protein in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.
    Depositor
    Mark Howarth
    Article
    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-
    Insert
    AviTag-DogTag-MBP
    Use
    Tags
    His6 and Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Peptide tag added to N-terminus of MBP via a GSGE…
    Promoter
    T7
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171773-plasmid-map-sequence-id-338517

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  12. pMIG-hNFATc2/C(1-686)AVITEV

    Plasmid
    #74054
    Purpose
    retroviral expression plasmid for human NFATc2/C (without C-terminal region) with C-terminal BirA-biotinylation signal and TEV celavage site
    Depositor
    Ria Baumgrass
    Article
    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.
    Insert
    human NFATc2, isoform C (NFATC2 Human, AS 1-686 (N terminus deleted))
    Use
    Retroviral
    Tags
    AVI-TEV
    Expression
    Mammalian
    Mutation
    silent mutation A1723C in the sequence of NM_1730…
    Promoter
    pMSCV-LTRs
    Available since
    June 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    74054-plasmid-map-sequence-id-142271

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  13. pBAD-6xHis-AviTag-ALFAtag-eGFP

    Plasmid
    #195854
    Purpose
    Bacterial expression vector encoding 6xHis-AviTag-ALFAtag-eGFP. Co-expression in cells with biotin ligase (BirA) results in efficient biotinylation of the AviTag sequence.
    Depositor
    John Ngo
    Article
    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103
    Insert
    6xHis-AviTag-ALFAtag-eGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    195854-plasmid-map-sequence-id-383965

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  15. pMIG-hNFATc2/C(RIT)-FLAG-AVITEV

    Plasmid
    #74053
    Purpose
    retroviral expression plasmid for human NFATc2/C (with RIT mutation abrogating AP1 binding) with C-terminal BirA-biotinylation signal and TEV celavage site
    Depositor
    Ria Baumgrass
    Article
    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.
    Insert
    human NFATc2, isoform C (NFATC2 Human)
    Use
    Retroviral
    Tags
    AVI-TEV and FLAG
    Expression
    Mammalian
    Mutation
    silent mutation A1723C in the sequence of NM_1730…
    Promoter
    pMSCV-LTRs
    Available since
    June 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    74053-plasmid-map-sequence-id-142273

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  22. pD649-HAsp-KIR2DS1-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156675
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    KIR2DS1 (KIR2DS1 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    156675-plasmid-map-sequence-id-383691

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  25. pD649-HAsp-SCN1B-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156534
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    SCN1B (SCN1B Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    Aug. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    156534-plasmid-map-sequence-id-368609

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  26. pD649-HAsp-IL1R1-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156756
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    IL1R1 (IL1R1 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156756-plasmid-map-sequence-id-404140

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  28. pD649-HAsp-CD22-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156940
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    CD22 (CD22 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    Jan. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    156940-plasmid-map-sequence-id-401025

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  29. pD649-HAsp-IL11RA-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156788
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    IL11RA (IL11RA Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156788-plasmid-map-sequence-id-426815

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  30. pD649-HAsp-SEMA4D-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156952
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    SEMA4D (SEMA4D Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    Jan. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    156952-plasmid-map-sequence-id-303013

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  31. pD649-HAsp-PDCD1LG2-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156612
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    PDCD1LG2 (PDCD1LG2 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156612-plasmid-map-sequence-id-302333

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  34. pD649-HAsp-CEACAM4-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156496
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    CEACAM4 (CEACAM4 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    Aug. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    156496-plasmid-map-sequence-id-368821

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  35. pD649-HAsp-IL1RAPL1-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156778
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    IL1RAPL1 (IL1RAPL1 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156778-plasmid-map-sequence-id-401004

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  37. pD649-HAsp-AXL-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156855
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    AXL (AXL Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156855-plasmid-map-sequence-id-302819

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  39. pD649-HAsp-CLEC7A-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156586
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    CLEC7A (CLEC7A Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156586-plasmid-map-sequence-id-302281

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  40. pD649-HAsp-HLA-B-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156835
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    HLA-B (HLA-B Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156835-plasmid-map-sequence-id-302779

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Showing: 121 - 160 of 25904 results