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Showing: 151 - 200 of 11158 results

  1. pJK137

    Plasmid
    #73313
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with c-myc tag and His6-tag
    Depositor
    T. J. Kappock
    Insert
    delta-12 fatty acid acetylenase
    Use
    Tags
    His6 and c-myc
    Expression
    Yeast
    Mutation
    synthetic gene
    Promoter
    5'-AOX1
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73313-plasmid-map-sequence-id-135010

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  2. pJK108

    Plasmid
    #73312
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2/synthetic)
    Depositor
    T. J. Kappock
    Insert
    delta-12 fatty acid acetylenase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73312-plasmid-map-sequence-id-135009

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  4. pET24a MDH_CHLP8

    Plasmid
    #204242
    Purpose
    Bacterial expression of C. parvum (green sulfur photosynthetic bacterium) malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDH_CHLP8
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204242-plasmid-map-sequence-id-413340

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  5. pET24a MDH_STRAW

    Plasmid
    #204240
    Purpose
    Bacterial expression of S. avermitilis malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDH_STRAW (mdh Streptomyces avermitilis)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204240-plasmid-map-sequence-id-413348

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  7. pJK141

    Plasmid
    #73316
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with Pas2p signal sequence, c-myc tag, and His6-tag
    Depositor
    T. J. Kappock
    Insert
    delta-12 fatty acid acetylenase
    Use
    Tags
    His6, Pas2p signal sequence, and c-myc
    Expression
    Yeast
    Mutation
    synthetic gene
    Promoter
    AOX1
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73316-plasmid-map-sequence-id-135024

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  8. pET28a MDHC_YEAST

    Plasmid
    #204232
    Purpose
    Bacterial expression of yeast cytosolic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDHC_YEAST (MDH2 Budding Yeast)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204232-plasmid-map-sequence-id-413337

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  9. pET24a MDHC1_ARATH

    Plasmid
    #204237
    Purpose
    Bacterial expression of Arabidopsis cytosolic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDHC1_ARATH (AT1G04410 Mustard Weed)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204237-plasmid-map-sequence-id-413345

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  10. pET28a MDH_PFALCI

    Plasmid
    #204235
    Purpose
    Bacterial expression of unicellular protozoan parasite P. falciparum malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDH_PFALCI (PF3D7_0618500 Plasmodium falciparum)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204235-plasmid-map-sequence-id-413338

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  11. pJK142

    Plasmid
    #73317
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) as a fusion to Pas2p, with c-myc tag and His6-tag
    Depositor
    T. J. Kappock
    Insert
    delta-12 fatty acid acetylenase
    Use
    Tags
    His6, Pas2p, and c-myc
    Expression
    Yeast
    Mutation
    synthetic gene. Encodes a glycine-204 to serine m…
    Promoter
    AOX1
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73317-plasmid-map-sequence-id-140432

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  12. pET24a MDH_HALMA

    Plasmid
    #204241
    Purpose
    Bacterial expression of H. marismortui (Dead Sea halophilic red archaeon) cytosolic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDH_HALMA (mdh Haloarcula marismortui)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204241-plasmid-map-sequence-id-413344

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  13. Clim Act MCH (CAM)

    Plasmid
    #104443
    Purpose
    Expresses mCherry under endogenous actin promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.
    Depositor
    Elena Casacuberta, Inaki Ruiz-Trillo
    Insert
    mCherry
    Use
    Expression in corallochytrium (single-celled euka…
    Tags
    Expression
    Mutation
    Promoter
    actin
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    104443-plasmid-map-sequence-id-200869

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  14. Clim EF1 MCH (CEM)

    Plasmid
    #104444
    Purpose
    Expresses mCherry under elongation factor 1 promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.
    Depositor
    Elena Casacuberta, Inaki Ruiz-Trillo
    Insert
    mCherry
    Use
    Expression in corallochytrium (single-celled euka…
    Tags
    Expression
    Mutation
    Promoter
    EF1
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    104444-plasmid-map-sequence-id-200864

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  15. Clim Tub MCH (CTM)

    Plasmid
    #104445
    Purpose
    Expresses mCherry under tubulin promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.
    Depositor
    Elena Casacuberta, Inaki Ruiz-Trillo
    Insert
    mCherry
    Use
    Expression in corallochytrium (single-celled euka…
    Tags
    Expression
    Mutation
    Promoter
    tubulin promoter
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    104445-plasmid-map-sequence-id-200865

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  21. pRL488

    Plasmid
    #70263
    Purpose
    designed to facilitate transcriptional fusions to luxAB, encoding luciferase, on a plasmid that can replicate in both Escherichia coli and strains of filamentous cyanobacteria.
    Depositor
    Peter Wolk
    Article
    Elhai et al EMBO J. 1990 Oct;9(10):3379-88.
    Type
    Empty backbone
    Use
    Luciferase; Cyanobacteria expression
    Tags
    luxAB
    Expression
    Mutation
    Promoter
    none
    Available since
    Nov. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    70263-plasmid-map-sequence-id-130206

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  22. pJK372

    Plasmid
    #73597
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-15 fatty acid desaturase (FAD3) with C-terminal c-myc epitope
    Depositor
    T. J. Kappock
    Inserts
    delta-12 fatty acid acetylenase
    delta-15 fatty acid desaturase
    Use
    Tags
    FLAG tag and c-myc tag
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 and GAL10
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73597-plasmid-map-sequence-id-138754

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  23. pJK370

    Plasmid
    #73595
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-2) with C-terminal c-myc epitope
    Depositor
    T. J. Kappock
    Inserts
    delta-12 fatty acid acetylenase
    delta-12 fatty acid desaturase (form 2)
    Use
    Tags
    FLAG tag and c-myc tag
    Expression
    Yeast
    Mutation
    Silent mutation in Arg299 codon.
    Promoter
    GAL1 and GAL10
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    73595-plasmid-map-sequence-id-138462

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  25. pET28a MDHM_YEAST

    Plasmid
    #204231
    Purpose
    Bacterial expression of yeast mitochondrial malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDHM_YEAST (MDH1 Budding Yeast)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Does not include transit peptide and includes a M…
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204231-plasmid-map-sequence-id-413335

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  29. pET28a-Ec.coaA

    Plasmid
    #50386
    Purpose
    Heterologous expression of E. coli CoA biosynthetic enzyme, PanK (pantothenate kinase), induced with IPTG. Contain 6xHis-tag for nickel affinity purification.
    Depositor
    Erick Strauss
    Article
    Brand et al J Biol Chem. 2005 May 27;280(21):20185-8
    Insert
    PanK
    Use
    Tags
    6XHis-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 13, 2014
    Availability
    Academic Institutions and Nonprofits only
    50386-plasmid-map-sequence-id-75331

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  33. pQE60 MGH

    Plasmid
    #204244
    Purpose
    Bacterial expression of a fusion of watermelon glyoxysomal malate dehydrogenase (MDH) and GFP with His tag; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MGH
    Use
    Tags
    6X His and GFP
    Expression
    Bacterial
    Mutation
    fusion of MDH and GFP with His tag
    Promoter
    Lac
    Available since
    Oct. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    204244-plasmid-map-sequence-id-411051

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  41. pfMDH_WT

    Plasmid
    #67721
    Purpose
    Expresses WT mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Use
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  48. pJK371

    Plasmid
    #73596
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-3) with C-terminal c-myc epitope
    Depositor
    T. J. Kappock
    Inserts
    delta-12 fatty acid acetylenase
    delta-12 fatty acid desaturase (form 3)
    Use
    Tags
    FLAG tag and c-myc tag
    Expression
    Yeast
    Mutation
    Point mutants in FAD2-3 ORF relative to deposited…
    Promoter
    GAL1 and GAL10
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73596-plasmid-map-sequence-id-139406

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  50. pfMDH_H303A

    Plasmid
    #67730
    Purpose
    Expresses H303A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Use
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed histidine 303 to alanine
    Promoter
    T7
    Available since
    Sept. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 151 - 200 of 11158 results