Addgene

We narrowed to 1,071 results for: PCS2

Showing: 151 - 200 of 1071 results

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pCS2_Firefly_degron-60A

Plasmid

#200396

Purpose

A plasmid IVT template containing a destabilized Firefly luciferase-PEST (degron) fusion followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.

Depositor

Xiao Wang

Article

Aditham et al ACS Chem Biol. 2022 Dec 16;17(12):3352-3

Insert

Firefly luciferase - degron

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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E12 2/5-pCS2

Plasmid

#20913

Depositor

Stephen Tapscott

Article

Yang et al Genes Dev. 2009 Mar 15. 23(6):694-707.

Insert

E12 2/5 (TCF3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pCS2z-tal1

Plasmid

#164656

Purpose

for the synthesis of zebrafish tal1 mRNA

Depositor

Didier Stainier

Article

Mattonet et al Sci Adv. 2022 Sep 2;8(35):eabn2082. doi:

Insert

tal1 (tal1 Zebrafish)

Use

Mrna synthesis

Tags

Expression

Mutation

Promoter

SP6

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2 xBtg-X

Plasmid

#24964

Depositor

Edward De Robertis

Article

Kuroda et al Genes Dev. 2005 May 1. 19(9):1022-7.

Insert

Btg-X (MGC76328.S Frog)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
pCS2-ER-EmEGFP

Plasmid

#169000

Purpose

Express ER-EmEGFP in zebrafish

Depositor

Noboru Mizushima

Article

Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

Insert

ER-EGFP (encoding the signal sequence of human calreticulin mRNA and EGFP-KDEL) (CALR Synthetic, Human)

Use

Unspecified

Tags

EGFP

Expression

Mutation

Promoter

Available since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+5'MT-wbp2nl

Plasmid

#172467

Purpose

expresses myc-tagged version of wild-type protein

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

wbp2nl

Use

Xenopus expression

Tags

Myc

Expression

Mutation

Promoter

SP6

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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LRP6 dC-pCS2

Plasmid

#27258

Depositor

Xi He

Article

Tamai et al Nature. 2000 Sep 28. 407(6803):530-5.

Insert

low density lipoprotein receptor-related protein 6 (LRP6 Human)

Use

Xenopus injection

Tags

Expression

Mammalian

Mutation

carboxyl intracellular region deleted Residue 14…

Promoter

Available since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only
pCS2TAL3-DDD

Plasmid

#48637

Purpose

Expression of TALEN proteins with FokI-DDD domain

Depositor

David Grunwald

Article

Hu et al PLoS One. 2013 May 7;8(5):e63074. doi: 1

Type

Empty backbone

Use

TALEN

Tags

Flag

Expression

Mutation

Promoter

CMV

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pCS2TAL3-RRR

Plasmid

#48636

Purpose

Expression of TALEN proteins with FokI-RRR domain

Depositor

David Grunwald

Article

Hu et al PLoS One. 2013 May 7;8(5):e63074. doi: 1

Type

Empty backbone

Use

TALEN

Tags

HA-1 and HA-2

Expression

Mutation

Promoter

CMV

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-HA-Mettl3

Plasmid

#196262

Purpose

In vitro transcription/translation of HA-tagged wild-type Mettl3

Depositor

Laura Attardi

Article

Raj et al Mol Cell. 2022 Jul 7;82(13):2370-2384.e1

Insert

Mettl3 (Mettl3 Mouse)

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-hSeparase-wt

Plasmid

#33016

Depositor

Marc Kirschner

Article

Stemmann et al Cell. 2001 Dec 14;107(6):715-26.

Insert

Separase (ESPL1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

simian CMV IE94

Available since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
pCS2 xCrescent-HA

Plasmid

#24697

Depositor

Edward De Robertis

Article

Pera et al Mech Dev. 2000 Sep . 96(2):183-95.

Insert

Crescent

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
Axin/pCS2HA

Plasmid

#16305

Depositor

Peter Klein

Article

Hedgepeth et al Mech Dev. 1999 Feb . 80(2):147-51.

Insert

Axin (axin1.L Frog)

Use

Vertebrate expression

Tags

HA

Expression

Mutation

Promoter

Available since

Dec. 21, 2007

Availability

Academic Institutions and Nonprofits only
pCS2+MT-hSeparase

Plasmid

#33017

Depositor

Marc Kirschner

Article

Stemmann et al Cell. 2001 Dec 14;107(6):715-26.

Insert

Separase (ESPL1 Human)

Use

Tags

6xMyc

Expression

Mammalian

Mutation

Promoter

simian CMV IE94

Available since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
Axin/pCS2MT

Plasmid

#16298

Depositor

Peter Klein

Article

Hedgepeth et al Mech Dev. 1999 Feb . 80(2):147-51.

Insert

Axin (axin1.L Frog)

Use

Vertebrate expression

Tags

5x myc

Expression

Mutation

Promoter

Available since

Dec. 21, 2007

Availability

Academic Institutions and Nonprofits only
pCS2 DN-xIGFR

Plasmid

#24699

Depositor

Edward De Robertis

Article

Pera et al Mech Dev. 2002 Aug . 116(1-2):169-72.

Insert

Dominant negative xIGFR (igf1r Frog)

Use

Tags

Expression

Mammalian

Mutation

C-terminal truncated version of the Xenopus IGF t…

Promoter

Available since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
kermit-2/pCS2+

Plasmid

#16314

Depositor

Peter Klein

Article

Wu et al Development. 2006 Sep . 133(18):3651-60.

Insert

Kermit 2 (gipc2 Frog)

Use

Vertebrate expression

Tags

Expression

Mutation

Promoter

Available since

Dec. 21, 2007

Availability

Academic Institutions and Nonprofits only
pCS2 xFrzb-HA

Plasmid

#24979

Depositor

Edward De Robertis

Article

Leyns et al Cell. 1997 Mar 21. 88(6):747-56.

Insert

Frzb-1 HA

Use

Tags

HA tag internal (YPYDVPDYA)

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pCS2 xBicaudal-C

Plasmid

#24959

Depositor

Edward De Robertis

Article

Wessely et al Development. 2000 May . 127(10):2053-62.

Insert

Bicaudal-C (bicc1-b Frog)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
pCS2+ HA BEX3

Plasmid

#231004

Purpose

Transient overexpression of human BEX3 in mammalian cells

Depositor

Michael Rape

Article

Manford et al Cell. 2021 Oct 14;184(21):5375-5390.e16.

Insert

BEX3 (BEX3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+ HA-BEX1

Plasmid

#231002

Purpose

Transient overexpression of human BEX1 in mammalian cells

Depositor

Michael Rape

Article

Manford et al Cell. 2021 Oct 14;184(21):5375-5390.e16.

Insert

BEX1 (BEX1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+ HA-BEX4

Plasmid

#231005

Purpose

Transient overexpression of human BEX4 in mammalian cells

Depositor

Michael Rape

Article

Manford et al Cell. 2021 Oct 14;184(21):5375-5390.e16.

Insert

BEX4 (BEX4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2TAL3-RR-zrif1_exon8

Plasmid

#194434

Purpose

Right (RR) TALEN for mutating zebrafish rif1 gene

Depositor

Christopher Sansam

Article

Masser et al eLife 2023 (Reviewed Preprint) 12:RP8767

Insert

zrif1_ex8_RIGHT (rif1 Zebrafish)

Use

TALEN

Tags

Expression

Mutation

Promoter

CMV IE94

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2TAL3-DD-zrif1_exon8

Plasmid

#194435

Purpose

Left (DD) TALEN for mutating zebrafish rif1 gene

Depositor

Christopher Sansam

Article

Masser et al eLife 2023 (Reviewed Preprint) 12:RP8767

Insert

zrif1_ex8_LEFT (rif1 Zebrafish)

Use

TALEN

Tags

Expression

Mutation

Promoter

CMV IE94

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-foxd4l1.1-QR

Plasmid

#185520

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

Expression

Mutation

replaces Q>R upstream of GARQYLNI

Promoter

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-foxd4l1.1-LA

Plasmid

#185518

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

Expression

Mutation

replaces L>A upstream of GARQYLNI

Promoter

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-foxd4l1.1-F>E

Plasmid

#185508

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

Expression

Mutation

F>E

Promoter

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2z-etsrp

Plasmid

#164655

Purpose

for the synthesis of zebrafish etsrp mRNA

Depositor

Didier Stainier

Article

Mattonet et al Sci Adv. 2022 Sep 2;8(35):eabn2082. doi:

Insert

etsrp (etsrp Zebrafish)

Use

Mrna synthesis

Tags

Expression

Mutation

Promoter

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2z-lmo2

Plasmid

#164657

Purpose

for the synthesis of zebrafish lmo2 mRNA

Depositor

Didier Stainier

Article

Mattonet et al Sci Adv. 2022 Sep 2;8(35):eabn2082. doi:

Insert

lmo2 (lmo2 Zebrafish)

Use

Mrna synthesis

Tags

Expression

Mutation

Promoter

SP6

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-foxd4l1.1-A6

Plasmid

#185506

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

Expression

Mutation

FSIENIM to AAAAAAM

Promoter

Available since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-hFAM3B-HA

Plasmid

#180145

Purpose

Coding sequence of human FAM3B pCS2 with C terminal HA tag

Depositor

Edward De Robertis

Article

Zhang et al Proc Natl Acad Sci U S A. 2021 May 18;11

Insert

FAM3B (FAM3B Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-Bighead S-FC

Plasmid

#180147

Purpose

mRNA synthesis for injection into embryos

Depositor

Edward De Robertis

Article

Ding et al Proc Natl Acad Sci U S A. 2018 Sep 25;11

Insert

Bighead.S

Use

Tags

FC

Expression

Mammalian

Mutation

Promoter

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+WBP2NL-HA

Plasmid

#174791

Purpose

Xenopus laevis WBP2NL with 3'HA tag

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

WBP2 N-terminal like (wbp2nl.L Frog)

Use

Tags

HA

Expression

Bacterial

Mutation

aa#165, Y to H; aa# 287, D to H

Promoter

SP6

Available since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2 Bighead S-HA

Plasmid

#172485

Purpose

for synthesis of mRNA for injection into xenopus laevis embryos

Depositor

Edward De Robertis

Article

Ding et al Proc Natl Acad Sci U S A. 2018 Sep 25;11

Insert

Bighead.S

Use

In vitro mrna synthesis

Tags

HA and S-HA

Expression

Mutation

Promoter

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-xFAM3b.L-HA

Plasmid

#172852

Purpose

mRNA synthesis for injection into embryos

Depositor

Edward De Robertis

Article

Ding et al Proc Natl Acad Sci U S A. 2018 Sep 25;11

Insert

xFAM3B.L (fam3b.L Frog)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2plus-TDP-43

Plasmid

#162608

Purpose

Allows for transcription of TDP-43 mRNA for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

TARDBP (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-F1-GGC

Plasmid

#30702

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F1 GGC

Use

Mrna production

Tags

FLAG

Expression

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pCS2_F1-GTT

Plasmid

#30708

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F1 GTT

Use

Mrna production

Tags

FLAG

Expression

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pCS2-F2-ACG

Plasmid

#30714

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F2 ACG

Use

Mrna production

Tags

Flag

Expression

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pCS2-F2-GCA

Plasmid

#30720

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F2 GCA

Use

Mrna production

Tags

HA

Expression

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pCS2-F2-GCG

Plasmid

#30722

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F2 GCG

Use

Mrna production

Tags

HA

Expression

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pCS2-F2-GCT

Plasmid

#30723

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F2 GCT

Use

Mrna production

Tags

FLAG tag

Expression

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pCS2-F2-GGC

Plasmid

#30725

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F2 GGC

Use

Mrna production

Tags

FLAG

Expression

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pCS2-F2-TGG

Plasmid

#30731

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F2 TGG

Use

Mrna production

Tags

FLAG

Expression

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pCS2-F3-GAT

Plasmid

#30739

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F3 GAT

Use

Mrna production

Tags

FLAG

Expression

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pCS2-F3-GCT

Plasmid

#30741

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F3 GCT

Use

Mrna production

Tags

FLAG tag

Expression

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pCS2-F3-GGT

Plasmid

#30743

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F3 GGT

Use

Mrna production

Tags

Flag

Expression

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pCS2-F3-GTG

Plasmid

#30746

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F3 GTG

Use

Mrna production

Tags

HA

Expression

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pCS2-F3-TGT

Plasmid

#30750

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F3 TGT

Use

Mrna production

Tags

FLAG

Expression

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pCS2-F2-GGG

Plasmid

#30752

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F2 GGG

Use

Mrna production

Tags

FLAG

Expression

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only

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