We narrowed to 59,693 results for: %s

1. Nsp3 Mac1 - P43 (Oxford)

   Plasmid

   #169210

   Purpose

   Expression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removal

   Depositor

   James S Fraser

   Article

   Schuller et al Sci Adv. 2021 Apr 14;7(16). pii: 7/16/ea

   Insert

   SARS-CoV-2 Nsp3 Mac1
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 28, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pmU1-CasRx-non-targeting (pKW83)

   Plasmid

   #229051

   Purpose

   Cloning backbone for CasRx crRNA (Mouse U1 promoter)

   Depositor

   S Chul Kwon

   Article

   Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XHA

   Plasmid

   #217511

   Purpose

   KD Recombinase dependent conditional smGdP-10XHA cassette

   Depositor

   S. Lawrence Zipursky

   Article

   Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

   Insert

   KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XHA
   

   Use

   Tags

   smGdP-10XHA

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEGFP-N1-mt-ro1GFP

   Plasmid

   #82407

   Purpose

   Expression of redox sensitive GFP with a mitochondrial targeting sequence in mammalian cells

   Depositor

   S. James Remington

   Article

   Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

   Insert

   ro1GFP
   

   Use

   Tags

   leader sequence of the E1α subunit of pyruvate de…

   Expression

   Mammalian

   Mutation

   C48S/F64L/T65S/S147C/Q204C

   Promoter

   CMV

   Available Since

   Sept. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. ICP8-P2A/Crimson (pSLIK4)

   Plasmid

   #113859

   Purpose

   For Doxycycline-inducible expression of ICP8 synaptase gene, with ICP8-P2A-red fluorescent protein gene E2-Crimson

   Depositor

   Richard S Myers

   Article

   Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

   Insert

   ICP8
   

   Use

   Lentiviral

   Tags

   P2A/Crimson

   Expression

   Mutation

   Promoter

   Available Since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJFRC-10XUAS-FRT-STOP-FRT-myrGFP-2A-KDR::Pest

   Plasmid

   #217513

   Purpose

   Encodes a UAS controlled and Flp dependent conditional trangene of bicistronic myrGFP and KD Recombinase

   Depositor

   S. Lawrence Zipursky

   Article

   Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

   Insert

   10XUAS-FRT-STOP-FRT-myrGFP-2A-KDR::Pest
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRSETB-de4GFP

   Plasmid

   #85472

   Purpose

   Inducible expression of dual emitting pH sensitive GFP in bacterial cells

   Depositor

   S. James Remington

   Article

   Hanson et al Biochemistry. 2002 Dec 31;41(52):15477-8

   Insert

   de4GFP
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   S65T/C48S/H148C/T203C

   Promoter

   T7

   Available Since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEGFP-N1-ro2GFP

   Plasmid

   #82370

   Purpose

   Expression of redox sensitive GFP in mammalian cells

   Depositor

   S. James Remington

   Article

   Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

   Insert

   ro2GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C48S/F64L/S147C/Q204C

   Promoter

   CMV

   Available Since

   Nov. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. eGFP-CBP

   Plasmid

   #169211

   Purpose

   Expression of eGFP-CBP protein for chitin visualization

   Depositor

   James S Fraser

   Article

   Ricardo-Gonzalez et al bioRxiv 2021.03.29.437438

   Insert

   Chitinase A1 Chitin Binding Domain
   

   Use

   Tags

   6xHis, Mxe Gyr Intein, and eGFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRSETB-ro2GFP

    Plasmid

    #82366

    Purpose

    Inducible expression of redox sensitive GFP in bacteria

    Depositor

    S. James Remington

    Article

    Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

    Insert

    ro2GFP
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    C48S/S65T/Q80R/S147C/Q204C

    Promoter

    T7

    Available Since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pQE30-roGFP1-iL

    Plasmid

    #83313

    Purpose

    Inducible expression of redox sensitive GFP in bacteria

    Depositor

    S. James Remington

    Article

    Lohman et al Biochemistry. 2008 Aug 19;47(33):8678-88

    Insert

    roGFP1-iL
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    ro1(C48S/S147C/Q204C) plus X insertion, iL betwe…

    Promoter

    T5

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pEGFP-N1-mt-ro2GFP

    Plasmid

    #82408

    Purpose

    Expression of redox sensitive GFP with a mitochondrial targeting sequence in mammalian cells

    Depositor

    S. James Remington

    Article

    Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

    Insert

    ro2GFP
    

    Use

    Tags

    leader sequence of the E1α subunit of pyruvate de…

    Expression

    Mammalian

    Mutation

    C48S/F64L/S147C/Q204C

    Promoter

    CMV

    Available Since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAF124

    Plasmid

    #222372

    Purpose

    Recombinant human IgG1 antibody (3D5) that binds to 6xHis at the C-terminus of proteins

    Depositor

    Andrew S. Flies

    Article

    Slyp et al Methods Mol Biol. 2024;2826:231-249. doi

    Insert

    IGHG
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Aug. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAF197

    Plasmid

    #135930

    Purpose

    All-in-one Sleeping Beauty vector for Expression for Tasmanian devil CTLA4-Fc-mCherry fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. CTLA4).

    Depositor

    Andrew S. Flies

    Article

    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab

    Insert

    CTLA4 fused to IgG Fc and to mCherry
    

    Use

    Transposon

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAF139

    Plasmid

    #135924

    Purpose

    All-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mTagBFP fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).

    Depositor

    Andrew S. Flies

    Article

    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab

    Insert

    TNFRSF9 fused to mTagBFP
    

    Use

    Transposon

    Tags

    mTagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAF164

    Plasmid

    #135928

    Purpose

    All-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mOrange fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).

    Depositor

    Andrew S. Flies

    Article

    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab

    Insert

    TNFRSF9 fused to mOrange
    

    Use

    Transposon

    Tags

    mOrange

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAF160

    Plasmid

    #135925

    Purpose

    All-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mAzurite fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).

    Depositor

    Andrew S. Flies

    Article

    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab

    Insert

    TNFRSF9 fused to mAzurite
    

    Use

    Transposon

    Tags

    mAzurite

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSG5-BMRF1

    Plasmid

    #72631

    Purpose

    Mammalian expression vector for Epstein-Barr virus polymerase accessory protein

    Depositor

    S. Diane Hayward

    Article

    Sarisky et al J Virol. 1996 Dec;70(12):8340-7.

    Insert

    BMRF1
    

    Use

    Tags

    D22G and S349N

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pRSETB-ro1GFP

    Plasmid

    #82365

    Purpose

    Inducible expression of redox sensitive GFP in bacteria

    Depositor

    S. James Remington

    Article

    Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

    Insert

    ro1GFP
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    C48S/Q80R/S147C/Q204C

    Promoter

    T7

    Available Since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Nsp3 Mac1 - C2 (UCSF)

    Plasmid

    #169209

    Purpose

    Expression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removal

    Depositor

    James S Fraser

    Article

    Schuller et al Sci Adv. 2021 Apr 14;7(16). pii: 7/16/ea

    Insert

    SARS-CoV-2 Nsp3 Mac1
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 28, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge